Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(4): 64-71

EFFECT OF N-ACETYLCARNOSINE CONTAINED EYE DROP ON CATECHOLAMINE-INDUCED MORPHOFUNCTIONAL DAMAGE IN RAT RETINA

V.V. Sanin1, A.I. Yakovets1, K.V. Rozova2, Yu.P. Korkach2, Yu.V. Goshovska2, I.V. Shargorodska3, S.O. Rykov3

  1. Kyiv City Clinical Ophthalmic Hospital Center for Eye Microsurgery, Kyiv, Ukraine
  2. O.O.Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine
  3. Shupyk National Medical Academy of Postgraduate Education of the Ministry of Health of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.04.064


Abstract

The effects of N-acetylcarnosine (NAC)-contained eye drop ‘Clarastil’ on a model of adrenaline-induced high intraocular pressure (IOP) in Wistar rats were studied. The retina ultrastructure and markers of oxidative stress have been studied. NAC was found to have no significant effect on edema in the retinal ultrastructure, did not reduce endothelial thickening and histogemic barrier, and accordingly did not affect the value of IOP after prolonged adrenaline administration. However, the introduction of the eye drop prevented the swelling of the mitochondria, the formation of vacuolated crystals and probably stimulated energy production as a compensatory mechanism under conditions of hypercatecholemia. In addition, NAC significantly reduced adrenaline-induced overproduction of reactive oxygen species and lipid peroxidation products in eye tissues, indicating its antioxidant effect.

Keywords: retina; adrenaline; intraocular pressure; oxidative stress; mitochondria, N-acetylcarnosine, glaucoma.

Full text: (PDF, in Ukrainian)

References

  1. Sovari AA, Dudley SC Jr. Reactive oxygen speciestargeted therapeutic interventions for atrial fibrillation. Front Physiol. 2012 Aug 6;3:311. CrossRef PubMed PubMedCentral
  2. S.O. Rykov, I.V. Shargorodska, K.V. Rozova, Yu.P. Korkach, Yu.V. Goshovska, V.V. Sanin, A.I. Yakovets, V.A. Dufynets. CATECHOLAMINE-INDUCED MORPHOFUNCTIONAL DISORDERS AND OXIDATIVE STRESS IN RAT RETINA. Fiziol. Zh. 2020; 66(2-3): 27-36 CrossRef
  3. Tamm ER., Schmetterer LE, Grehn FG. Status and perspectives of neuroprotective therapies in glaucoma: The European Glaucoma Society White Paper. Cell Tiss Res. 2013; 353(2): 347-54. CrossRef PubMed
  4. Moiseenko RO, Golubchikov MV, Slabkiy GO, Rykov SO. Oftalmologichna dopomoga v Ukraini za roki nezaleghnosti. Kyiv. 2019. 368p.
  5. Park H, Raffiee AH, John SWM, Ardekani AM, Lee H. Towards smart self-clearing glaucoma drainage device. Microsyst Nanoeng. 2018;4:35. CrossRef PubMed PubMedCentral
  6. Weinreb RN. Glaucoma neuroprotection: What is it? Why is it needed? Can J Ophthalmol. 2007;42:396-8. CrossRef PubMed
  7. Kaushik S, Pandav SS, Ram J. Neuroprotection in glaucoma. J Postgrad Med. 2003; 49: 90-5. CrossRef PubMed
  8. Barkana Y, Belkin M. Neuroprotection in ophthalmology: A review. Brain Res Bull. 2004;62:447-53. CrossRef
  9. Babizhayev MA, Burke L, Micans P, Richer SP. NAcetylcarnosine sustained drug delivery eye drops to control the signs of ageless vision: glare sensitivity, cataract amelioration and quality of vision currently available treatment for the challenging 50,000-patient population. Clin Interv Aging. 2009;4:31-50. CrossRef
  10. Boldyrev AA, Dupin AM, Bunin AYa, Babizhayev MA, Severin SE. The antioxidative properties of carnosine, a natural histidine containing dipeptide. Biochem Int. 1987;15:1105-13.
  11. Avetisov SÉ, Sheremet NL, Muranov KO, Polianskiĭ NB, Polunin GS, Ostrovskiĭ MA. Deceleration of cataract development in rats under the action of N-acetylcarnosine and D-pantethine mixture. Eksp Klin Farmakol. 2014;77(11):11-5.
  12. Shao L, Li QH, Tan Z. L-carnosine reduces telomere damage and shortening rate in cultured normal fibroblasts. Biochem. Biophys. Res. Commun. 2004; 324(2), 931-6. CrossRef PubMed
  13. Babizhayev MA; Yegorov YE. Telomere attrition in lens epithelial cells a target for N-acetylcarnosine therapy. Front Biosci. 2010;15:934-56. CrossRef PubMed
  14. Dubois VD, Bastawrous A. N-acetylcarnosine (NAC) drops for age-related cataract. Cochrane Database Syst Rev. 2017 Feb 28;2:CD009493. CrossRef PubMed PubMedCentral
  15. Budama-Kilinc Y, Cakir-Koc R, Kecel-Gunduz S, Kokcu Y, Bicak B, Mutlu H, E Ozel A. Novel NAC-loaded poly(lactide-co-glycolide acid) nanoparticles for cataract treatment: preparation, characterization, evaluation of structure, cytotoxicity, and molecular docking studies. PeerJ. 2018 Jan 30;6:e4270. CrossRef PubMed PubMedCentral
  16. Wang Y, Xia R, Hu H, Peng T. Biosynthesis, characterization and cytotoxicity of gold nanoparticles and their loading with N-acetylcarnosine for cataract treatment. J Photochem Photobiol B. 2018 Oct;187:180-3. CrossRef PubMed
  17. Mikheytseva IM. Protective effect of melatonin on experimental glaucoma in rats. Fiziol Zh. 2013;59(1):78-83. CrossRef
  18. Wickley B. Electron microscopy for beginners. M: World. 1975.
  19. Karupu VYa. Electron microscopy. K: Vish School. 1984.
  20. Eglen SJ, Weeks M, Jessop M. Simonotto J, Jackson T, Sernagor E. Data repository and analysis framework for spontaneous neural activity recordings in developing retina. Gigascience 2014; 3(1):3. CrossRef PubMed PubMedCentral
  21. Weibel ER. Human lung morphometry M.: Medicine. 1970. 170p.
  22. Kuthan H, Ullrich V, Estabrook RW. A quantitative test for superoxide radicals produced in biological systems. Biochem J. 1982;203(3): 551-8. CrossRef PubMed PubMedCentral
  23. Humphries KM, Yoo Y, Szweda LI. Inhibition of NADHlinked mitochondrial respiration by 4-hydroxy-2-nonenal. Biochem. 1998;37(2): 552-7. CrossRef PubMed
  24. Gavrilov VB, Gavrilova AR, Khmara NF. Measurement of diene conjugates in blood plasma using the UV absorption of heptane and isopropanol extracts. Lab Delo. 1988;(2): 60-4.
  25. Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978;86(1): 271-8. CrossRef
  26. Sudakova YV, Bakeyeva LY, Tsiplenkova VG. Energozavisimye izmeneniy aul'trastruktury mitokhondriy kardiomiotsitov cheloveka pri alkogol'nom porazhenii serdtsa. Arkhiv Patologii. 1999;2:15-20.
  27. Novoselov VP, Savchenko SV, Porvin AN, Koshlyak DA, Nadev AP, Ageeva TA, Chikinev YV, Polyakevich AS. Ultrastructure of Cardiomyocytes and Blood Capillary Endotheliocytes in the Myocardium under Conditions of Experimental Mechanical Injury to the Heart. Bull Exp Biol Med. 2016 May;161(1):134-6. CrossRef PubMed
  28. Antonova EI. Ultrastructural manifestations of the primary compensatory-adaptive response of hepatocytes in animals with various thermoregulation after exposure to hyperthermia. Morfologiya. 2008;133(4):24-8.
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.