Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(4): 46-54


DYSLIPIDEMIA AND REPRODUCTIVE DISORDERS IN MALE RATS DUE TO PROLONGED CONSUMPTION OF HYDROGENATED PALM OIL

R.V. Antipova

    H.S. Skovoroda Kharkiv National Pedagogical University, Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz66.04.046

Abstract

The paper focuses on the problem of unbalanced nutrition. The effects of hydrogenated palm oil (solid form) consumption at a dose of 3 g / kg body weight, which exceeded the daily norm by 1,5 times, for 75 days in adult male rats have been experimentally shown. The simulated experimental conditions led to the development of dyslipidemia in the serum and the liver (hypertriglyceridemia, hypercholesterolemia). Signs of androgen deficiency were also found: the content of total and free testosterone in the serum was significantly less than the physiological norm. Also, defects in spermatogenesis were recorded in the experimental animals: total gametes concentration and proportion of morphologically normal gametes decreased, the pathological forms increased. Disorders of androgen-synthetic and spermatogenic functions in the testes were accompanied by histological changes. In testicular tissues, structural rearrangements of a number of seminal tubules have been observed, possibly due to an increase in inactive Leydig cell forms. A decrease in the index of spermatogenesis was found, this reflects the number of generations of spermatogenic cells in the wall of the seminal tubules. An increased number of tortuous seminal tubules with exfoliated germ cells in the lumen have been reported. The reason may be the loss of communication between the germ cells and the supporting Sertoli cells. When the link between the germ cell and the supporting cells is lost, the effects of testosterone are impossible, which leads to a violation of spermatogenesis.

Keywords: dyslipidemia; hydrogenated palm oil; sex hormones; reproduction of male rats.

References

  1. Smolyar VI. The concept of ideal fat nutrition. Nutrition problems. 2006;4: 5-13. [Ukrainian].
  2. Bankovska NV. Hygienic assessment of the actual nutrition of the adult population of Ukraine and scientific substantiation of ways to optimize it. Author's Ref. Cand. Med. Sci. Kyiv. 2008; 14.02.01 hygiene and occupational pathology. National Medical University. O. O. Bogomolets of the Ministry of Health of Ukraine: 26. [Ukrainian].
  3. Ruiz-Samblás C, Arrebola-Pascual C. Authentication of geographical origin of palm oil by chromatographic fingerprinting of triacylglycerols and partial least squarediscriminant analysis. Talanta. 2013;116:788-93. CrossRef PubMed
  4. Natrus LV, Gayova LV, Byhovets YuM, et al. The value of regulatory effects on lipid metabolism in during complicated diabetes mellitus. Fiziol Zh. 2020; 66(1):25-34. [Ukrainian]. CrossRef
  5. Gregory HN, Christopher NB. Dietary and Endogenous sphingolipid metabolism in chronic inflammation. Nutrients. 2017;9(11):1180. CrossRef PubMed PubMedCentral
  6. Romanenko MS. Nutrition, metabolism and biological rhythms. Nutrition problems. 2014;2:5-14. URI: http:. nbuv.gov.ua. UJRN. [Ukrainian].
  7. Karpenko NO, Talko VV, Omelchuk ST, Lapta SS. Integral assessment of the reproductive function of male laboratory animals. Ukr Biopharm Zh. 2011;13(2):64-8. [Ukrainian].
  8. Merkulov GA. Course of pathological and histological technique. M.: Medicine. 1969:424. [Russian].
  9. Barylyak IR, Neumerzhytska LV, Byshovets TF, Danylenko BC. Study of gonadotoxic effects of new drugs and their effects on reproductive function of animals. In the book: Preclinical studies of drugs (guidelines). Ed. by O. V. Stefanova. K.: Avicenna. 2001:139-52. [Ukrainian].
  10. Lapach SN, Chubenko AV, Babich PN. Statistical methods in medical and biological research using Ehsel. K.: Morion. 2001:320. [Ukrainian].
  11. Rebrova OYu. Statistical analysis of medical data. Application package application STATISTICA. M.: Media Sphere. 2006: 312. [Russian].
  12. Sayapina IYu. Quantitative analysis of the functional activity of the testis of rats under oxidative stress induced by adaptation to low temperatures. Bulletin of new medical technologies. 2011;18(2):155-7. [Russian].
  13. Go RE, Hwang KA, Kim YS, et al. Effects of palm and sunflower oils on serum cholesterol and fatty liver in rats. J Med Food. 2015;18 (3):363-9. CrossRef PubMed
  14. Fattore E, Bosetti C, Brighenti F, et al. Palm oil and blood lipid-related markers of cardiovascular disease: a systematic review and meta-analysis of dietary intervention trials. Am J Clin Nutr. 2014;99(6):1331-5. CrossRef PubMed
  15. Bu X, Niu D, Wu J, et al. Elevated levels of preβ1- high-density lipoprotein are associated with cholesterol ester transfer protein, the presence and severity of coronary artery disease. Lipids in Health and Disease. 2017;16(4):1-7. CrossRef PubMed PubMedCentral
  16. Habib A, Mihas A. A, Abou-Assi SG, et al. High-density lipoprotein cholesterol as an indicator of liver function and prognosis in noncholestatic cirrhotics. Clin Gastroenterol Hepatol. 2005;3(3):286-91. CrossRef
  17. Coleman J, Rem KG. Visual biochemistry. M.: Mir. 2009:473. [Russian].
  18. Van Capelleveen JC, Kastelein JJ, Zwinderman AH, et al. Effects of the cholesteryl ester transfer protein inhibitor, TA-8995, on cholesterol efflux capacity and highdensity lipoprotein particle subclasses. J Clin Lipidol. 2016;10(5):1137-44. CrossRef PubMed
  19. Fortunati N. Sex hormone-binding globulin: not only a transport protein. What news is around the corner? J Endocrinol Invest. 1999;22(3):223-34. CrossRef PubMed
  20. Dohle GR, Arver S, Bettocchi C, et al. Recommendations for male hypogonadism. Translation: KA Shiranov. Scientific editing: ME Chaly. Eur Associat Urologists. 2014:28.
  21. Potemina TE. Disruption of spermatogenesis under stress in male rats. Bull Exp Biol and Med. 2008;145(6): 645-7. [Russian]. CrossRef PubMed
  22. Tash JS, Johnson DC, Enders GC. Long-term (6-wk) hindlimb suspension inhibits spermatogenesis in adult male rats. J Appl Physiol. 2002;92(3):1191-8. CrossRef PubMed
  23. Kartashev AG. Influence of chronic factors in postnatal ontogenesis of animals. Tomsk: V-Spectrum. 2010:116. [Russian].
  24. Kidun KA, Doroshenko RV, Ugolnik TS. Morphological changes of testicular tissues in male outbred white rats under acute mobilization stress. Health and Environmental Issues. 2013;3 (37):97-101. [Russian].

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.