Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(4): 37-45


THE EFFECT OF STRESS AND PHYTOESTROGENIZATION IN THE NEONATAL PERIOD ON THE FERTILITY OF ADULT MALE RATS

N.F. Velichko, N.O. Karpenko, E.M. Koreneva, E.E. Chistyakova, N.P. Smolenko, V.О. Bondarenko

    State Institution "V. Danilevsky Institute for Endocrine Pathology Problems of the NAMS of Ukraine", Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz66.04.037

Abstract

In the paper the significance of milk-feeding period for the formation of male reproductive health is experimentally shown. It has been found that the use of emotional stress and excessive phytoestrogenization, both separately and together, in male rats during breastfeeding leads to certain disorders of the reproductive system in adulthood. Modeled emotional stress or phytoestergenization on the principle of imprinting caused hyperostrogeny, androgen deficiency, changes in the ratio of androgenization/estrogenization in adulthood. The applied factors led to impaired spermatogenesis, inhibition of sexual activity, and decreased reproductive potential. In the stressed animals, the decrease in androgens was such that the testosterone levels did not differ from intact females. In the case of joint application of the factors in the suckling period, in adult male rats there were no clinically significant changes in the sperm count. The concentration of sex hormones corresponded to the physiological norm, sexual behavior was characterized by slightly differentiated changes. However, a 2.5-fold decrease in their fertility (or potential number of offspring) was critical and largest among all study groups. The latter indicates impaired spermatogenesis and the formation of defective sperm, that is, the problem of the parental genome, possibly epigenetic in nature.

Keywords: emotional stress; phytoestrogens; breastfeeding; male fertility; spermogram; sex hormones.

References

  1. Neumann ID, Torner L, Wigger A. Brain oxytocin: differential inhibition of neuroendocrine stress responses and anxiety-related behavior in virgin, pregnant and lactating rats. J Neurosci. 2000;95:567-75. CrossRef
  2. Catalani A, Alemà GS, Cinque CC, et al. Maternal corticosterone effects on hypothalamus-pituitary-adrenal axis regulation and behavior of the offspring in rodents. J Neurosci Biobehav Rev. 2011;35(7):1502-17. CrossRef PubMed
  3. Levine S. Regulation of the hypothalamic-pituitaryadrenal axis in the neonatal rat: the role of maternal behavior. Neurotox Res. 2002;4(5-6):557-64. CrossRef PubMed
  4. Pawluski JL, Lambert KG, Kinsley CH. Neuroplasticity in the maternal hippocampus: Relation to cognition and effects of repeated stress. Horm Behav. 2016;77:86-97. CrossRef PubMed
  5. Reznikov AG. Functional teratology of the neuroendocrine system: etiology, pathogenesis, prevention. Health Ukraine. 2007;22(1):19-21. [Ukrainian].
  6. Moles A, Rizzi R, D'Amato FR. Postnatal stress in mice: does "stressing" the pups? J Dev Psychobiol. 2004;44(4):230-7. CrossRef PubMed
  7. Walker CD, Deschamps S, Proulx K, et al. Mother to infant to mother? Reciprocal regulation of responsiveness to stress in rodents and implications for humans. J Y Psychiat Neurosci. 2004;29(5):382-91.
  8. Litvitsky PF. Clinical pathophysiology: a textbook. M.: Practical medicine. 2015:776. [Russian].
  9. Miodovnik A, Engel SM, Zhu C, et al. Endocrine disruptors and childhood social impairment. Neurotoxicology. 2011;32(2):261-7. CrossRef PubMed PubMedCentral
  10. Ball ER, Caniglia MK, Wilcox JL, Burr J, et al. Effects of genistein in the maternal diet on reproductive development and spatial learning in male rats. Horm Behav. 2010;57(3):313-22. CrossRef PubMed PubMedCentral
  11. Jochum F, Alteheld B, Meinardus P. Mothers' consumption of soy drink but not black tea increases the flavonoid content of term breast milk: a pilot randomized, controlled intervention study. Ann Nutr Metab. 2017;70(2):147-53. CrossRef PubMed
  12. Hoey L, Rowland IR, Lloyd AS, et al. Influence of soyabased infant formula consumption on isoflavone and gut microflora metabolite concentrations in urine and on faecal microflora composition and metabolic activity in infants and children. Br J Nutr. 2004;91(4):607-16. CrossRef PubMed
  13. Setchell KD, Zimmer-Nechemias L, Cai J, Heubi JE. Isoflavone content of infant formulas and the metabolic fate of these phytoestrogens in early life. Am J Clin Nutr. 1998;68 (6):1453-61. CrossRef PubMed
  14. Cao YA, Calafat AM, Doerge DR, et al. Isoflavones in urine, saliva, and blood of infants: data from a pilot study on the estrogenic activity of soy formula. J Exp Sci Environ Epidemiol. 2008;30:225-35.
  15. Gladkova AI, Yaremenko FG, Nikishina LYe, Kravchenko SV. The study of the composition of the soybean product genistein soy complex by chromatographic methods. Advances and prospects of experimental and clinical endocrinology (Tenth Danile Readings): Mater. Research Practice Conf. from the international. participation, Kharkiv 3-4 March, 2011:29-30. [Ukrainian].
  16. Owens W, Ashby J, Odum J, et al. The OECD program to validate the rat uterotrophic bioassay. Phase 2: dietary phytoestrogen analyses. Environ Helth Perspect. 2003;111(12):1559-67. CrossRef PubMed PubMedCentral
  17. Karpenko NO, Talko VV, Omelchuk ST, Lapta SS. Integral assessment of the reproductive function of male laboratory animals. Ukr Biopharm Zh. 2011;13(2):64-8. [Ukrainian].
  18. Petrishchev VS, Schelochkov AM. Assessment of sperm morphology according to strict criteria (literature review). Prob Reproduct. 2002;(3):87-91. [Ukrainian].
  19. Mohort TV, Zabarovskaya ZV, Shepelkevich AP. Clinical endocrinology: textbook. allowance. Minsk: Higher education School. 2013:415. [Belarus].
  20. Handa RJ, Weiser M. Gonadal steroid hormones and the hypothalamo-pituitary-adrenal axis. Front Neuroendocrin. 2014;(16):67-72.
  21. McHenry J, Carrier N, Hull E, et al. Sex differences in anxiety and depression: Role of testosterone. Front Neuroendocrin. 2014; 35(1):42-57. CrossRef PubMed PubMedCentral
  22. Keen-Rhinehart E, Michopoulos V, Toufexis DJ, et al. Continuous expression of corticotropin-releasing factor in the central nucleus of the amygdala emulates the dysregulation of the stress and reproductive axes. J Mol Psychiat. 2009;14(1):37-50. CrossRef PubMed PubMedCentral
  23. Karpenko NO, Somova OV, Koreneva EM, et al. Hormonal changes in adult rats exposed to stress and. or phytoestrogens during breastfeeding. Endocrinology. 2011;16 (1):76-82. [Ukrainian].
  24. Emelyanov VYu. Morphological changes of the uterus under the action of estrogens under conditions of prolonged increase of glucocorticoid hormone concentration: author. Dis. … cand. med. sci: 03.00.25; CSU them. I.N. Ulyanov; Moscow. 2007:26. [Russian].

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.