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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(4): 12-19


LARINGOPHARINGEAL REFLUX IMPACTS IMMUNE MICROENVIRONMENT OF LARYNGEAL CARCINOMA

D.I. Zabolotnyi1, V.V. Kizim1, D.D. Zabolotna1, Y.V. Kizim1, O.N. Sulaieva2

  1. A.S. Kolomiychenko State Institution "Institute of Otolaryngology NAMS of Ukraine”, Kyiv, Ukraine
  2. Medical Laboratory CSD, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.04.012

Abstract

The purpose of this study was to evaluate the effect of the laryngopharyngeal reflux (LPR) on the number of tumourinfiltrating T-lymphocytes in laryngeal cancer (LC). According to the results of pH monitoring, 87 patients with laryngeal tumours were subdivided into three groups: 1st group included patients with LC without LPR; 2nd group comprised LC patients with coexisting LPR, patients with benign neoplasms of the larynx with LPR were enrolled into 3d group. TIME was assessed immunohistochemically by counting T-lymphocytes (CD3+), T-cytotoxic cells (CD8+) and T-regulatory cells (Treg; FOXP3+) number within the tumour, in the peritumour stroma, and in the intact areas of the larynx. It was shown that LPR leads to chronic inflammation and affects TIME of laryngeal carcinomas. LC with coexisting LPR demonstrated a higher inflammatory infiltration of tumour area and intact mucosa. However, no statistically significant differences were found between a number of CD3+- and CD8+-cells in LC of the 1st and 2nd groups. In contrast, LPR was associated with higher number of immunosuppressive Treg-cells within tumour and in intact mucosa that could affect immune tolerance and efficacy of anti-tumour immunity facilitating LC progression.

Keywords: : laryngeal cancer; laryngopharyngeal reflux; tumour immune microenvironment; T-lymphocytes; T-regulatory cells

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