Possibilities of AV reconstruction with autologous tissues using mathematical calculations reported by Gasparyan were evaluated. We also assessed efficacy of neo-AV functioning and designed new instruments capable of simplifying individual parameters measurements and enabling correct intraoperative AV leaflets tailoring in time-saving manner. We have performed series of 10 experiments on porcine hearts in the Wetlab of Government Institution «The ScientificPractical Children’s Cardiac Center» of Health Ministry of Ukraine (Kyiv, Ukraine) aiming to study opportunities of AV re-construction. Set of sizers and templates were designed according to Gasparian’s calculations. Instruments were manufactured by “DM Med-service” Ltd (Kyiv, Ukraine) as an experimental set. Hydrodynamic conditions of AV functioning were simulated via water pumping into aortic root through sutured-in vascular prosthesis. Invasive hydrodynamic parameters’ recording with IntelliVue X2 (“Phillips”, Netherlands) were applied for AV reconstruction results assessment. Thoracoscopic video-recording of neoAV was carried out using WideView (“Storz”, Germany) advanced into vascular prosthesis. Series of experiments have shown good AV competence and capability of neo-AV to resist high pressures, similar to those in natural conditions of AV functioning. Lack of pressure drop and significant leakage during simulated diastole characterize reconstructed with autologous pericardium porcine AV as competent in all 10 experiments. Designed and manufactured set of sizers and templates enables efficacious AV reconstruction in vitro. Implementation of neo-cuspidization using the set of sizers enables time-friendly precise intraoperative measurements and tailoring of neo-AV cusps.