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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(2-3): 27-36


CATECHOLAMINE-INDUCED MORPHOFUNCTIONAL DISORDERS AND OXIDATIVE STRESS IN RAT RETINA

S.O. Rykov1, I.V. Shargorodska1, K.V. Rozova2, Yu.P. Korkach2, Yu.V. Goshovska2, V.V. Sanin3, A.I. Yakovets3, V.A. Dufynets4

  1. PL Shupyk National Medical Academy of Postgraduate Education, Kyiv, Ukraine
  2. Bogomoletz Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine
  3. Kyiv City Clinical Ophthalmic Hospital Center for Eye Microsurgery, Ukraine
  4. Vizis exclusive Ophthalmic Center, Mukachevo, Ukraine
DOI: https://doi.org/10.15407/fz66.2-3.027

Abstract

The effects of adrenaline administration for 40 days (0.1-0.15 mg/kg i/p ones in two days) on intraocular pressure (IOP), ultrastructure and markers of oxidative stress in the retina of 10 months old Wistar rats were studied. The IOP values were found to increase significantly after adrenaline administration by 21% (P<0.03) with a sustained long-term effect even 42 days after the last injection. Adrenaline caused destructive changes of retinal neurons and cell mitochondria of all layers: the average number of structurally damaged mitochondria increased in 7.6 times, and the average diameter of mitochondria increased by 78.4%. Swelling in the retinal ultrastructure, endothelium and histogematic barrierthickening were observed which extend the distance of oxygen diffusion and may be a prerequisite for tissue hypoxia and glaucoma. In addition, adrenaline caused an increase in the production of reactive oxygen species and lipid peroxidation in the tissues of the eye which indicates the development of oxidative stress during long-term adrenaline administration. Thus, morphological and biochemical changes in the retina have been identified which may explain the elevated IOP during prolonged action of adrenaline. The correction of adrenaline-induced disturbances in the retina should include the use of antioxidants, mitochondrial and endothelial membrane protective drugs.

Keywords: retina; adrenaline; intraocular pressure; oxidative stress; mitochondria.

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