L-CYSTEINE STIMULATES ENDOGENOUS HYDROGEN SULFIDE SYNTHESIS, SUPPRESSES OXIDATIVE STRESS AND MITOCHONDRIAL PERMEABILITY TRANSITION PORE OPENING IN THE HEART OF OLD RATS
N.A. Strutynska, Yu.P. Korkach, L.А. Mys, А.Yu. Luchkova, V.F. Sagach
- Bogomoletz Institute of physiology NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.2-3.003

Abstract
The effect of the amino acid L-cysteine on the hydrogen sulfide
content, oxidative stress indicators and kinetic characteristics
of Ca2+-induced mitochondrial swelling in the heart of aged
rats were investigated. Single administration to old rats of
L-cysteine (121 mg/kg) was shown to suppress spontaneous
and Ca2+-induced swelling of mitochondria, which manifested
in a decrease in the amplitude of these processes and indicated
prevention of mPTP formation in the heart. During aging, the
mitochondrial pools of hydrogen sulfide decreased against the
background of violation of constitutive NO synthesis, and also
a significant increase of inducible and salvage NO synthesis.
The use of L-cysteine resulted in an increase of 2.56-fold the
content of hydrogen sulfide in the mitochondria of the heart of
old rats (6.015 ± 0,74 nmol/mg protein) compared to animals
without the introduction of the amino acid (2.35 ± 0,25 nmol/
mg protein). Hence, under these conditions, the synthesis of
hydrogen sulfide in the mitochondria was restored, probably
due to the activation of H2S-synthesizing enzymes by their
substrate, which contributed to significant inhibition of
inducible NO synthesis by 1.73 times and a decrease by 2 times
of nitrate reductase and arginase activity. The introduction of
L-cysteine to old animals significantly (1.5 times) reduced
uric acid pools, which may indicate suppression of superoxide
radicals formation, and significantly decreased pools of diene
conjugates (7 times), which indicates the suppression of oxidative
stress in the early stages of lipid peroxidation. Thus, the
results of studies indicate the participation of L-cysteine in
the stimulation of endogenous hydrogen sulphide synthesis in
mitochondria, which contributes to the reduction of oxidative
stress in organelles and modulation of changes in the permeability
of mitochondrial membranes. This fact speaks to the
importance of such a gas transmitter as hydrogen sulphide,
as a regulatory factor in the cardiovascular system, which
modulates the functions of cells and their organelles.
Keywords:
L-cysteine; hydrogen sulphide; oxidative stress; nonspecific mitochondrial pore; aging.
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