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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(6): 94-104


THE ROLE OF NF-κB IN THE DIFFERENTIATION AND ACTIVATION OF NEUTROPHILS DURING THE BURN WOUND HEALING OF THE SKIN IN RATS

L.V. Natrus1, I.M. Ryzhko1, O.O. Lisakovska2, A.O. Tykhomyrov2, D.O. Labudzinskyi2, T.I. Panova3

  1. Research Institute of Experimental and Clinical Medicine Bogomolets National Medical University, Kyiv, Ukraine
  2. Palladin Institute of Biochemistry NAS of Ukraine, Kyiv, Ukraine
  3. Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz65.06.094

Abstract

We investigated the link between levels of key regulatory pro-inflammatory proteins (nuclear factor kappa B (NF-κB), it’s inhibitory subunit κB-α (IκB-α), vascular endothelial growth-factor A (VEGF-A) and endothelial receptor FLT1) in a connective tissue regenerate and changes in bone marrow granulocyte and peripheral blood neutrophils counts during the skin burn wound healing (on the 3,7,14,21st day) in Wistar rats. It was detected a correlation between the NF- κB contents and the granulocytes count r=0.739 (P <0.01); between the neutrophils count and the VEGF-A contents r = 0.708 (P<0.01); between the FLT1contents and the VEGF-A contents r = 0.865 (P <0.01); between the neutrophils contents and the FLT1 contents (r = 0.472, P <0.05). The obtained data provided the basis for formation of a hypothetical multi-level regulatory chain in the system of connective tissue - bone marrow - peripheral blood, appearing under the normal physiological condition and possible key mechanism of angiogenesis regulation. NF-κB probably activates the production of myeloid progenitor growth factors and regulates the differentiation of granulocytes in the bone marrow; neutrophils from the blood enter the damaged area of the burn wound, secrete endothelial growth factors and stimulate the expression of endothelial receptors to these ligands in the connective tissue regenerate.

Keywords: skin regeneration, wound, bone marrow, neutrophils, angiogenesis.

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