THE ROLE OF NF-κB IN THE DIFFERENTIATION AND ACTIVATION OF NEUTROPHILS DURING THE BURN WOUND HEALING OF THE SKIN IN RATS
L.V. Natrus1, I.M. Ryzhko1, O.O. Lisakovska2, A.O. Tykhomyrov2, D.O. Labudzinskyi2, T.I. Panova3
- Research Institute of Experimental and Clinical Medicine
Bogomolets National Medical University, Kyiv, Ukraine
- Palladin Institute of Biochemistry NAS of Ukraine, Kyiv, Ukraine
- Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz65.06.094
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Abstract
We investigated the link between levels of key regulatory
pro-inflammatory proteins (nuclear factor kappa B (NF-κB),
it’s inhibitory subunit κB-α (IκB-α), vascular endothelial
growth-factor A (VEGF-A) and endothelial receptor FLT1)
in a connective tissue regenerate and changes in bone marrow
granulocyte and peripheral blood neutrophils counts during
the skin burn wound healing (on the 3,7,14,21st day) in
Wistar rats. It was detected a correlation between the NF-
κB contents and the granulocytes count r=0.739 (P <0.01);
between the neutrophils count and the VEGF-A contents r =
0.708 (P<0.01); between the FLT1contents and the VEGF-A
contents r = 0.865 (P <0.01); between the neutrophils contents
and the FLT1 contents (r = 0.472, P <0.05). The obtained
data provided the basis for formation of a hypothetical
multi-level regulatory chain in the system of connective
tissue - bone marrow - peripheral blood, appearing under the
normal physiological condition and possible key mechanism
of angiogenesis regulation. NF-κB probably activates the
production of myeloid progenitor growth factors and regulates
the differentiation of granulocytes in the bone marrow;
neutrophils from the blood enter the damaged area of the
burn wound, secrete endothelial growth factors and stimulate
the expression of endothelial receptors to these ligands in the
connective tissue regenerate.
Keywords:
skin regeneration, wound, bone marrow, neutrophils, angiogenesis.
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