Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(6): 38-42


K.O. Drachuk1, Yu.P. Korkach1, N.A. Dorofeyeva1, O.I. Kornelyuk2, V.F. Sagach1

  1. O.O. Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. Institute of Molecular Biology and Genetics, NAS of Ukraine, Kyiv, Ukraine


We studied the effect of endothelial monocyte-activating polypeptide-II (EMAP II) on endothelium-dependent vascular relaxation in old rats. It was found a violation of endotheliumdependent aorta relaxation in these animals, the average value of its amplitude was 7.5±1.4% compared with 64.9±3.5% in healthy adult rats. EMAP II significantly improved endothelium-dependent relaxation in old animals, the average value of its amplitude increased to 66.6±6.8%. This effect was due to oxidative stress inhibition, cNOS recoupling and increasing of constitutive NO-synthesis; thus, index of cNOS coupling/ uncoupling increased by 51 times and cNOS activity increased by 2.8 times.

Keywords: endothelial monocyte-activating polypeptide-II; aging; endothelium-dependent relaxation; oxidative stress; nitric oxide


  1. Francis SH, Busch JL, Corbin JD, Sibley D. cGMPdependent protein kinases and cGMP phosphodiesterases in nitric oxide and cGMP action. Pharmacol Rev. 2010;62(3):525-63. CrossRef PubMed PubMedCentral
  3. Van Hove CE, Van der Donckt C, Herman AG, Bult H, Fransen P. Vasodilator efficacy of nitric oxide depends on mechanisms of intracellular calcium mobilization in mouse aortic smooth muscle cells. Br J Pharmacol. 2009;158(3):920-30. CrossRef PubMed PubMedCentral
  5. El Assar M, Angulo J, Vallejo S, Peiró C, Sánchez-Ferrer CF, Rodríguez-Mañas L. Mechanisms involved in the aging-induced vascular dysfunction. Front Physiol. 2012;28;3:132. CrossRef PubMed PubMedCentral
  7. Dorofeyeva NA, Kotsuruba AV, Mogilnitskaya LA, Malyna AE, Kornelyuk AI, Sagach VF. Endothelial monocyteactivating factor II cancels oxidative stress, constitutive NOS uncoupling and induced violations of cardiac hemodynamica in hypertension (part II). Fiziol Zh. 2014;61(3):11-8. [Ukrainian].
  9. Dorofeyeva NA, Vorobyov GG, Cherepaha IY, Kornelyuk AI, Sagach VF. Endothelial monocyte-activating polypeptide-II improves diastolic heart function and vascular relaxation in streptozotocin-induced experimental diabetes. Fiziol Zh. 2017;63(6):3-8. CrossRef  
  10. Drachuk KO, Kotsjuruba AV, BazilyukOV, Stepanenko LG, Sagach VF. Propargylglycine restores endotheliumdependent relaxation of aortic smooth muscle in old rats. Fiziol Zh. 2014;60(4):3-10 [Ukrainian].
  12. Dubrovsky AL, Brown D, Kornelyuk AI, Murray DK, Matsuka GC. Bacterial expression of full-length and truncated forms of thecytokine EMAP-2 and the cytokinelike domain of mammalian tyrosyl-tRNA synthetase. Biopolym and Cell. 2000;16 (3):229-35. CrossRef  
  13. Sharipov RR, Kotsiuruba AV, Kop'iak BS, Sahach VF. Induction of oxidative stress in heart mitochondria in brain focal ischemia-reperfusion and protective effect of ecdysterone. Fiziol Zh. 2014;60(3):11-7. [Ukrainian]. CrossRef PubMed
  15. Tkachenko MN, Sagach VF, Kotsjuruba AV, Baziljuk OV, Buchanevich AM, Meged EF, et al. Endothelium-dependent contractile reactions of vascular smooth muscles and content of free radicals of oxygen of rats in aging. Fiziol Zh. 2002;48(4):3-13. [Ukrainian].
  17. Vásquez-Vivar J, Kalyanaraman B, Martásek P. The role of tetrahydrobiopterin in superoxide generation from eNOS: enzymology and physiological implications. Free Radic Res. 2003;37(2):121-7. CrossRef PubMed
  19. Galougahi KK, Liu CC, Gentile C, Kok C, Nunez A, Garcia A, et al. Glutathionylation mediates angiotensin II-induced eNOS uncoupling, amplifying NADPH oxidase-dependent endothelial dysfunction. J Am Heart Assoc. 2014;3(2):e000731. CrossRef PubMed PubMedCentral
  20.   Hill BG, Bhatnagar A. Protein S-glutathiolation: redoxsensitive regulation of protein function. J Mol Cell Cardiol. 2012;52(3):559-67 CrossRef PubMed PubMedCentral  
  21. Brandes RP, Fleming I, Busse R. Endothelial aging. Cardiovasc Res. 2005;66(2):286-94. CrossRef PubMed

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.