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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(6): 22-29


M.V. Telka, V.Yu. Maslov, S.A. Fedulova, N.S. Veselovsky

    Bogomoletz Institute of Physiology of NAS of Ukraine, Kyiv, Ukraine


Noradrenaline action on electrical activity of cultured trigeminal ganglion neurons (TGN) was studied. Noradrenaline (NA) application on an isolated sensory neuron simulates sympathetic-sensory influences on signal transmission via primary trigeminal afferents. In response to depolarization the neurons exhibited three types of activity: tonic (68%, n = 52), adaptive (28%, n=21) and delayed-generation (4%, n=3). Under the NA application, part of tonic neurons (25%, n=13) lost the tonic firing ability; also, reducing of action potential (AP) (from 93±5 mV to 68±4 mV) and afterhyperpolarization (from -28±2 mV to -22±2 mV) amplitude was observed. In 46% (n=24) of tonic neurons, AP amplitude (from 98±2 mV до 86±3 mV) and repolarization phase duration (from 3.2±0.2 ms to 2.6±0.2 ms) were decreased. In adaptive and delayedgeneration neurons, the NA application led to a reducing in AP amplitude (from 91±1mV to 76 ±2 mV; n=17) and repolarization phase duration (from 4.2±0.5 ms до 3.2±0.3 ms; n=17). When hyperpolarized, tonic and adaptive neurons demonstrated inward rectification; this effect was reduced under the influence of NA (the relative inward rectification coefficient values compared to control were 0.84 ± 0.1 and 0.76 ± 0.04 respectively). The data obtained suggests that adrenergic action on electrical activity of TGN is provided via adrenergic receptors influence on high-threshold voltage-gated calcium channels and hyperpolarization-activated channels.

Keywords: trigeminal ganglion neurons; primary culture; electrical activity; noradrenaline.


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