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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(5): 56-63


АNTIOXIDANT DEFENSE SYSTEM STATE IN THE LIVER AND THE MYOCARDIUM OF RATS UNDER CONDITIONS OF ACUTE HYPOXIA-HYPERCAPNIA

S.V. Khyzhnyak1, V.S. Morozovа1, S.V. Midyk1, T.V. Poltavchenko2, A.A. Kaplia1

  1. National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
  2. National University of Water Management and Environmental Management, Rivne, Ukraine
DOI: https://doi.org/10.15407/fz65.05.056

Abstract

The prooxidant-antioxidant state of rat tissues in the formation of adaptive processes in acute hypoxia-hypercapnia with lower body temperature was evaluated. The decrease of the content of 2-thiobarbituric acid reactive substances (TBARS) in liver and myocardial tissues by 48.2 and 51.0%, respectively is shown, it indicates the oxidative processes inhibition in the tissues. The liver superoxide dismutase (SOD) activity decreased by 24.2% mainly due to its Cu, Zn-SOD molecular form and catalase activity increased by 29.4%. In the myocardium the activity of SOD increased by 62.7% mainly due to Mn-SOD molecular form and catalase activity decreased by 21.9%. Тhe studied parameters return to the control values 24 h after withdrawal of the effect of the hypoxia-hypercapnia. It was shown that differently directed changes of the glutathione peroxidase activity and reduced glutathione content in tissues play a compensatory role in hypoxia-hypercapnia.It is concluded that during acute hypoxia-hypercapnia the course of oxidative processes in the rat’s body is controlled by the antioxidant defense system. The possible regulatory role of hypercapnia in these conditions is discussed.

Keywords: hypercapnia; hypoxia; liver; myocardium; superoxide dismutase; catalase; glutathione.

References

  1. Timofeev NN. Hypobiosis and cryobiosis: The past, present and future. Moscow: Inform-Znanie; 2005. [Russian].
  2.  
  3. Melnytchuk SD, Melnytchuk DO. The animal hypobiosis state (molecular mechanisms and practical implications for the agriculture and medicine). Kyiv: NULES press; 2007. [Ukrainian].
  4.  
  5. Kolomiytseva IK. Lipids in mammalian hibernation and artificial hypobiosis of mammals. Biochemistry (Mosc). 2011;76 (12):1291-9. CrossRef PubMed
  6.  
  7. Baraboi VA. Bioantioxidants. Kyiv: Kniga Plus; 2006. [Ukrainian].
  8.  
  9. Sena LA, Chandel NS. Physiological roles of mitochondrial reactive oxygen species. Mol Cell. 2012; 48(2): 158-67. CrossRef PubMed PubMedCentral
  10.  
  11. Gonchar OO, Mankovska IM. Mitochondrial thioldisulfide system for acute hypoxia and hypoxic-hyperoxic adaptation. Ukr Biochem J. 2014;86(1):93-100. [Ukrainian]. CrossRef  
  12. Melnytchuk SD, Khyzhnyak SV, Morozova VS, Stepanova LI, Umanskaya AA. The energy function of rat cardiac mitochondria under artificial hybobiosis. Fiziol Zh. 2015;61(2):15-22. [Ukrainian]. CrossRef PubMed
  13.  
  14. Stalnaya ID. Garishvili TG. Method for the determination of malonic dialdehyde using thiobarbituric acid. In: Modern methods in biochemistry. Moscow; 1977. [Russian].
  15.  
  16. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972; 247(10): 3170-5.
  17.  
  18. Koroliyuk MA, Ivanova LI, Maiorova IG, Tokarev VE. A method of determining catalase activity. Lab Delo. 1988; 1:16-9. [Russian].
  19.  
  20. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971; 44(1):276-87. CrossRef  
  21. Vlasova SN, Shabunina EI, Pereslegina IA. The activity of the glutathione-dependent enzymes of erythrocytes in chronic liver diseases in children. Lab Delo. 1990;8:19- [Russian].
  22.  
  23. Rahman I, Kode A, Biswas SK. Assay for quantitative determination of glutathione and glutathione disulphide levels using enzymatic recycling method. Nature Protocols. 2007;1:3159-65. CrossRef PubMed
  24.  
  25. Sanjuan-Pla A, Cervera AM, Apostolova N, Garcia-Bou R, Víctor VM, Murphy MP, McCreath KJ. A targeted antioxidant reveals the importance of mitochondrial reactive oxygen species in the hypoxic signaling of HIF- 1alpha. FEBS Lett. 2005;579(12):2669-74. CrossRef PubMed
  26.  
  27. Mel'nychuk SD, Kuz'menko AI, Margitich VM, Govseeva NN, Gorid'ko TN, Hulaia NM. Effect of carbon dioxide on free-radical processes as affected by artificial hypobiosis in rats. Ukr Biokhim Zh. 1998;70(1):87-94. [Russian].
  28.  
  29. Gudkova OO, Latyshko NV, Gudkova LV, Mikhailovsky VO. Rat liver catalase under artificial hypobiosis conditions. Biopolym Cell. 2005;21(1):28-34. [Ukrainian]. CrossRef  
  30. Fridovich I. Superoxide radical and superoxide dismutases. Annu Rev Biochem. 1995; 64:97-112. CrossRef PubMed
  31.  
  32. Astaeva MD, Klichkhanov NK. Oxidative modification of proteins and antioxidant activity of gopher blood during induced awakening from hibernation. Izvestiya RAN. Seriy biologycheskay. 2009. 6:662-8. [Russian]. CrossRef  
  33. Vesela A, Wilhelm J. The role of carbon dioxide in free radical reactions of the organism. Physiol Res. 2002;51(4):335-9.
  34.  
  35. Andrekopoulos C, Zhang H, Joseph J, Kalivendi S, Kalyanaraman B. Bicarbonate enhances α-synuclein oligomerization and nitration: intermediacy of carbonate radical anion and nitrogen dioxide radical. Biochem J. 2004; 378(Pt2):435-47. CrossRef PubMed PubMedCentral

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