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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(5): 49-55

The influence of melatonin on carbohydrate metabolism in muscles of alloxan diabetic rats

I.M. Yaremii1, O.Yu. Kushnir1, V.I. Shvets1, I.R. Yanchii2, N.V. Shvets1

  1. Higher Education Institution in Ukraine «Bukovinian State Medical University», Chernivtsi, Ukraine
  2. V.P. Komisarenko Institute of Endocrinology and Metabolism of NAMS Ukraine, Kyiv


The aim of this study was to determine the influence of melatonin on basal level of glucose in the blood (BG), glycogen content (GC), activities of glucose-6-phosphate dehydrogenase (G6PD), pyruvate kinase (PK) and lactate dehydrogenase (LDH) in muscles of alloxan diabetic rats under conditions of different photoperiod (artificial equinox, constant darkness and constant light). In the DM rats the LDH activity increased on average by 61%, whereas the GC and the activities of PK and G6PD decreased on average by 37, 53 and 40% respectively compared with control values. All of these changes were not dependent on the light conditions. The BG level of the IGT rats didn’t reliably differ from the control, however, the LDH and the G6PD activities were respectively higher on average by 24 and 49%, except rats under the constant light conditions whose G6PD activity was lower by 48%. A melatonin administration (14 daily injections of 10 mg/kg) led to an improvement of the carbohydrate metabolism: the BG level, the GC, the activities of PK and LDH were normalized, while the G6PG activity was increased by an average of 32%. The influence of melatonin was more prominent in the IGT rats under the constant light conditions.

Keywords: melatonin; alloxan diabetes; carbohydrate metabolism; muscles; rats.


  1. Nabavi SF, Habtemariam S, Daglia M, Sureda A, SobarzoSánchez E, Selamoglu Z, Gulhan MF, Nabavi SM. Melatonin and respiratory diseases: a review. Curr Top Med Chem. 2017;17(4):467-88. CrossRef PubMed
  3. Thomas AP, Hoang J, Vongbunyong K, Nguyen A, Rakshit K, Matveyenko AV. Administration of melatonin and metformin prevents deleterious effects of circadian disruption and obesity in male rats. Endocrinology. 2016;157(12):4720-31. CrossRef PubMed PubMedCentral
  5. Qian J, Block GD, Colwell CS, Matveyenko AV. Consequences of exposure to light at night on the pancreatic islet circadian clock and function in rats. Diabetes. 2013;62(10):3469-78. CrossRef PubMed PubMedCentral
  7. Marcheva B, Ramsey KM, Buhr ED, Kobayashi Y, Su H, Ko CH, Ivanova G, Omura C, Mo S, Vitaterna MH, Lopez JP, Philipson LH, Bradfield CA, Crosby SD, JeBailey L, Wang X, Takahashi JS, Bass J. Disruption of the clock components CLOCK and BMAL1 leads to hypoinsulinaemia and diabetes. Nature. 2010;466(7306):627-31. CrossRef PubMed PubMedCentral
  9. Krishna T, Yaremii I, Kushnir O. Protective effects of melatonin in alloxan diabetic rats under conditions of constant darkness. Book of abstracts of 24-th European Students' Conference ["Exploring the unknown"]. 2013; ESCID 396.
  11. Favero G, Rodella LF, Nardo L, Giugno L, Cocchi MA, Borsani E, Reiter RJ, Rezzani R. A comparison of melatonin and α-lipoic acid in the induction of antioxidant defenses in L6 rat skeletal muscle cells. Age (Dordr). 2015;37(4):9824. CrossRef PubMed PubMedCentral
  13. Kushnir OYu, Yaremii IM, Shvetsv VI, Shvets NV. Influence of melatonin on carbohydrate metabolism in the kidney of alloxan diabetic rats. Fiziol. Zh., 2017;63(4):64-71. [Ukrainian]. CrossRef  
  14. Ha E, Yim SV, Chung JH, Yoon KS, Kang I, Cho YH, Baik HH. Melatonin stimulates glucose transport via insulin receptor substrate-1. phosphatidylinositol 3-kinase pathway in C2C12 murine skeletal muscle cells. J Pineal Res. 2006;41(1):67-72. CrossRef PubMed
  16. Kushnir A., Yaremii I, Malinevskaya A. Impact of melatonin on the activity of pyruvate kinase in the liver of alloxan  diabetic and tetrachlormethane intoxicated rats exposed to light deprivation. Eur. Applied Sci. 2014;2:35-37.
  18. Gesmundo I, Villanova T, Banfi D, Gamba G, Granata R. Role of Melatonin, Galanin, and RFamide Neuropeptides QRFP26 and QRFP43 in the Neuroendocrine Control of Pancreatic β-Cell Function. Front Endocrinol (Lausanne). 2017;8:143. CrossRef PubMed PubMedCentral
  20. Lin GJ, Huang SH, Chen YW, Hueng DY, Chien MW, Chia WT, Chang DM, Sytwu HK. Melatonin prolongs islet graft survival in diabetic NOD mice. J Pineal Res. 2009 Oct;47(3):284-92. CrossRef PubMed
  22. Aly HF, Mantawy MM. Comparative effects of zinc, selenium and vitamin E or their combination on carbohydrate metabolizing enzymes and oxidative stress in streptozotocin induced-diabetic rats. Eur Rev Med Pharmacol Sci. 2012;16(1):66-78.
  24. Gerush I, Boichuk T, Yaremii I, Kushnir O, Gerush O. Effects of melatonin on the glutathione system in the blood of alloxan diabetic rats. The International Union of Biochemistry and Molecular Biology (IUBMB) and the Federation of European Biochemical Societies (FEBS). 2012;279(1):88.
  26. Kushnir A., Meshchyshen I, Yaremii I. Hypoglycemic and antioxidant action of melatonin in alloxan diabetic rats. Annales Universitatis Marie Curie Skladovska. 2010;23(3,31):227-30.
  28. Czuczejko J, Sielski Ł, Woźniak B, Woźniak A, SzewczykGolec K. Melatonin supplementation improves oxidative and inflammatory state in the blood of professional athletes during the preparatory period for competitions. Free Radical Res. 2019;53(2):198-209. CrossRef PubMed
  30. Brar J, Chhokar A. Kushnir. O. Influence of melatonin long term introduction on condition of the Langergans islets of the pancreas i alloxan diabetic rats. 22-nd Inter. Stud. Congress of biomedical sciences. Book of abstract. 2015; 544.
  32. Kushnir A, Davydenko I. Influence of melatonin on condition of the langergans isles of the pancreas in alloksan diabetic rats. World Med and Biol. 2009;5(4):31-5.
  34. Buonfiglio D, Parthimos R, Dantas R. Melatonin absence leads to long-term leptin resistance and overweight in rats front endocrinol (Lausanne). 2018;9:122. CrossRef PubMed PubMedCentral
  36. Oberlin D and Buettner C. How does leptin restore euglycemia in insulin-deficient diabetes? J Clin Invest. 2017;127(2):450-53. CrossRef PubMed PubMedCentral

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