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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(5): 40-48


O.Y. Isayenko1, O.V. Knysh1, O.V. Falko2, V.Y. Prokopyuk2, O.S. Prokopyuk2

  1. SE “I. Mechnikov Institute of Microbiology and Immunology of NAMS of Ukraine”, Kharkiv, Ukraine
  2. Institute for Problems of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine, Kharkiv, Ukraine


The effect of the structural-metabolic complexes of Lactobacillus rhamnosus and Saccharomyces boulardii on the viability of test cells – mouse embryo fibroblasts and mouse splenocytes – was investigated by their metabolic activity. Disintegrates, which are structural components, obtained through the low-frequency ultrasonic treatment of the probiotic cells of Lactobacillus rhamnosus GG or Saccharomyces boulardii. Metabolites obtained by the author’s method by cultivating lactobacilli and saccharomycetes in their own disintegrate, and their combination - in the disintegrate of lactobacilli. The cytotoxicity of disintegrate and metabolites was studied using tests in vitro characterizing the metabolic activity of cells. The content of the filtrate in the incubation medium at a concentration of 5% does not alter the metabolic activity of both studied types of test cells using different oxidative indicators (MTT and Alamar Blue®). The increase in the content of the studied filtrate in the incubation medium to 20% is accompanied by statistically significant inhibition of the metabolic activity of embryonic fibroblasts (from 56.19 ± 1.4 to 88.73 ± 8.6%) and mouse splenocytes (from 52.63 ± 0.7 to 80.5 ± 6.8%) depending on active cell-free product. The obtained results of the study of cytotoxicity and determination of the concentrationdependent nature of the influence of disintegrates and the metabolites of Lactobacillus rhamnosus and Saccharomyces boulardii, obtained by the author’s method without using traditional nutrient media, can be used to create antimicrobial agents of the new generation.

Keywords: embryonic fibroblasts; mouse splenocytes; MTT; Alamar Blue®; metabolic activity.


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