Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2002; 48(1): 25-29

Influence of adrenoceptor agonists and changesin intracellular cyclic nucleotides concentration on the membrane potential of isolatedendothelial cell layer from rabbit aorta

V.V.Yarotsky V.F. Sagach S.M.Marchenko

    A.A.Bogomoletz Institute of PhysiologyNational Academy of Science of Ukraine, Kiev



Abstract

The influence of adrenoceptor agonists and changes in the intracellular cyclic nucleotide concentration on the membrane potential of an isolated endothelial cell layer from rabbit aorta has been investigated. The β1-adrenoceptor agonist phenylephrine evoked deep depolarization of the endothelium of an intact aorta, but had a little effect on the membrane potential of the isolated endothelium. The nonselective adrenoceptor agonist noradrenaline, the β-adrenoceptor agonist isoproterenol and the inhibitor of phosphodiesterases IBMX all strongly depolarized the isolated endothelium. These data suggest that the rise in cyclic nucleotide concentration depolarizes endothelial cells, and this fact may account for the noradrenaline-induced depolarization of the endothelium through β-adrenoceptors.

Full text: (PDF, in Ukrainian)

References

  1. Шуба М.Ф., Кочемасова Н.Г. Физиология сосудистых гладких мышц. - К.: Наук. думка, 1998. –252 с.
  2. Яроцкий В.В., Сагач В.Ф., Марченко С.М. Влияние циклических нуклеотидов на мембранный потенциал интактного эндотелия изолированной аорты кролика. - В кн.: Функциональная роль монооксида азота и пуринов. - Минск.: Бизнесофсет, 2001. – 216 с.
  3. Яроцький В.В., Сагач В.Ф., Марченко С.М.Електричні властивості інтактного ендотелію аорти кроля // Фізіол.журн. – 2001. – 47. –С. 9-16
  4. Beny J-L. Electrical coupling between smooth muscle cells and endothelial cells in pig coronary arteries // Pflugers Archiv. – 1997. – 433. – P. 364-367.
  5. Emerson G.G., Segal S.S. Electrical coupling between endothelial cells and smooth muscle cells in hamster feed arteries: role of vasomotor control //Circulat.Res. – 2000. – 87(6). – P. 427-428.
  6. Feletou M., Vanhoutte P.M. The third pathway:endothelium-dependent hyperpolarisation //J. Physiol.Pharmacol. – 1999. – 50(4). – P.525-534.
  7. Hill C.E., Hickey H., Sandow S.L. Role of gap junction in acetylcholine –induced vasodilation of proximal and distal arteries of the rat mesentery// J.Auton.Nerv.Syst. – 2000. – 81(1-3). –P. 122-127.
  8. Marchenko S.M.,Sage S.O. Smooth muscle cellc affect endothelial membrane potential in rat aorta// Amer. J.Physiol. – 1994. – 267. – P. H804-H811.
  9. 9. Sandow S.L., Hill C.E. Incidence of myoendothelial gap junctions in the proximal and distal mesenteric arteries of the rat is suggestive of a role in endothelium-derived hyperpolarizing factor-mediated responses // Circulat. Res. – 2000. – 86(3). –P. 341-346.
  10. 10. Spagnoli L.G., Villaschi S., Neri L., Palmieri G.Gap junction in myo-endothelial bridges of rabbit carotid arteries // Experientia. – 1982. – 38. –P. 124-125.
  11. von der Weid P.-Y., Beny J.-L. Simultaneous oscillations in the membrane potential of pig coronary artery endothelial and smooth muscle cells// J.Physiol. – 1993. – 471. – P. 13-24.
  12. Yamamoto Y.,Fukuta H., Nakahira Y., Suzuki H.Blockade by 18b-glycyrrhetinic acid intercellular electrical coupling in guinea-pig arterioles //J.Physiol. – 1998. – 511, N 2. – P. 501-508.
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.