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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(5): 3-10


THE EFFECT OF THYROXINE AND ESTRADIOL ON THE BEHAVIOR, NEURAL STEM AND IMMUNE CELLS OF THE MOUSE BRAIN WITH A CUPRIZONE DEMYELINATION MODEL

I.F. Labunets, A.E. Rodnichenko, T.M. Panteleymonova

    State Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz65.05.003

Abstract

In young 129/Sv mice receiving neurotoxin cuprizone and thyroxin or estradiol we investigated the relationship between changes in behavioral indices and the number of neural stem cells, T-lymphocytes, microglia/macrophage cells in the brain assessed by nestin, CD3, Mac1 markers, as well as the number of latex phagocytic macrophages. Cuprizone was given with food daily for 3 weeks; L-thyroxin or β-estradiol were administered from the 8th day of the cuprizone diet, daily. It is shown that cuprizone significantly reduced motor, emotional and exploratory activities. Changes in behavioral indices coincided with an increase in the number of nestin+-, CD3+- and Mac1+-cells in the brain and active macrophages (1.6, 2.3, 1.5 and 1.2 times, respectively) comparing to the intact mice. In the thyroxin-treated mice the locomotor activity increased by 1.3 times, but it was lower comparing to the intact animals. І.Ф. Лабунець, А.Є. Родніченко, Т.М. Пантелеймонова Under the influence of thyroxin the number of nestin+-, Mac1+- cells and active macrophages decreased to the values of the intact group. After estradiol administration the locomotor and emotional activities increased 1.4 and 2.5 times, respectively, comparing to the cuprizone group. The number of nestin+-cells was higher in the group with estradiol than in the mice without it, while the number of active macrophages decreased and did not differ from the values of the intact group.Thus, in mice with the cuprizone demyelination model, the positive effect of thyroxin and estradiol on the CNS functioning was realized through the changes in the number of brain nestin+- cells and the factors of the pathology.

Keywords: сuprizone; thyroxin; estradiol; behavioral indices; neural stem cells; macrophages and T-lymphocytes, brain.

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