Peptidoglycane modulates rat myometrial contractility via Ca2+ release from SR
L.S. Nasibyan, I.B. Philyppov, Y.M. Shuba
Bohomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz65.04.066
Abstract
The aim of current trial was to investigate the SR role in the peptidoglycan elicitted alteration of myometrial
contractility. For the study we used peptidoglycan from Staphylococcus aureus. In order to assess the
peptidoglycan’s effect on myometrial contractility under different conditions we used the method of
tensometry and took into account the average amplitude, frequency and the area under the curve of the
myometrial contractions. The results of our experiments confirmed our suggestion that peptidoglycan
activates myometrial contractility due to the SR depletion via IP3 receptors activation as well as the
amplification of transmembrane Ca2+ influx. Under peptydoglycan the amplitude of myometrial contractions
increased by nearly 7,0 ± 0.346% [n=10] compared to the control. The area under the curve increased
by nearly 30,0 ± 0.256% due to both the amplitude and the duration of contractions. In our study we
demonstrated that peptidoglycan makes the myometrial strip to contract even in the Ca2+-free environment
throughout a few minutes. Also we demonstrated that phospholipase C blockade with U73122 doesn’t prevent
entirely the stimulation of myometrial contractions by peptidoglycan. Phospholipase C blockade resulted in
statistically unreliable increase in the amplitude of the peptydoglycan-stimulated myometrial contractions,
their frequency decreased by 47 ± 1.05% and the area under the curve of single contraction decreased by
nearly 56 ± 1.2%. But 2APB, a non selective IP3 antagonist, entirely reduced the peptidoglycan-stimulated. We suggested that peptidoglycan acts via several pathways simultaneously and Ca2+ release from SR via
IP3 receptors activation is among them.
Keywords:
peptidoglycane; myometrium; myometrial smooth muscle cells; myometrial contractile activity
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