Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(4): 66-72


Peptidoglycane modulates rat myometrial contractility via Ca2+ release from SR

L.S. Nasibyan, I.B. Philyppov, Y.M. Shuba

    Bohomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz65.04.066

Abstract

The aim of current trial was to investigate the SR role in the peptidoglycan elicitted alteration of myometrial contractility. For the study we used peptidoglycan from Staphylococcus aureus. In order to assess the peptidoglycan’s effect on myometrial contractility under different conditions we used the method of tensometry and took into account the average amplitude, frequency and the area under the curve of the myometrial contractions. The results of our experiments confirmed our suggestion that peptidoglycan activates myometrial contractility due to the SR depletion via IP3 receptors activation as well as the amplification of transmembrane Ca2+ influx. Under peptydoglycan the amplitude of myometrial contractions increased by nearly 7,0 ± 0.346% [n=10] compared to the control. The area under the curve increased by nearly 30,0 ± 0.256% due to both the amplitude and the duration of contractions. In our study we demonstrated that peptidoglycan makes the myometrial strip to contract even in the Ca2+-free environment throughout a few minutes. Also we demonstrated that phospholipase C blockade with U73122 doesn’t prevent entirely the stimulation of myometrial contractions by peptidoglycan. Phospholipase C blockade resulted in statistically unreliable increase in the amplitude of the peptydoglycan-stimulated myometrial contractions, their frequency decreased by 47 ± 1.05% and the area under the curve of single contraction decreased by nearly 56 ± 1.2%. But 2APB, a non selective IP3 antagonist, entirely reduced the peptidoglycan-stimulated. We suggested that peptidoglycan acts via several pathways simultaneously and Ca2+ release from SR via IP3 receptors activation is among them.

Keywords: peptidoglycane; myometrium; myometrial smooth muscle cells; myometrial contractile activity

References

  1. Arrowsmith S, Robinson H, Noble K, Wray S, J. What do we know about what happens to myometrial function as women age?. Muscle Res Cell Motil. 2012; 33: 209-17. CrossRef PubMed PubMedCentral
  2.  
  3. Oki T, Douchi T, Maruta K, Nakamura S, Nagata K. Changes in endometrial wave-like movements in accordance with the phases of menstrual cycle. J Obstet Gynaecol; 2002; 28: 176-81. CrossRef  
  4. Pinto V, Matteo M, Tinelli R, Mitola PC, Ziegler D De. Altered uterine contractility in women with chronic endometritis. Fertil Steril; 2015; 103 (4): 1049-52. CrossRef PubMed
  5.  
  6. Pellati D, Mylonakis I, Bertoloni G, Fiore C, Andrisani A, Ambrosini G, Armanini G. Genital tract infections and infertility. Eur J Obstet Gynecol Reprod Biol. 2008; 14: 3-11. CrossRef PubMed
  7.  
  8. Agrawal V, Hirsch E. Intrauterine infection and preterm labor. Semin Fetal Neonatal Med. 2012; 17(1): 12-9. CrossRef PubMed PubMedCentral
  9.  
  10. Ilievski V, Hirsch E. Synergy between viral and bacterial toll-like receptors leads to amplification of inflammatory responses and preterm labor in the mouse. Biol Reprod. 83(5): 767-73. CrossRef PubMed PubMedCentral
  11.  
  12. Wheeler RI, Chevalier G, Eberl G, Gomperts Boneca I. The biology of bacterial peptidoglycans and their impact on host immunity and physiology. Cell Microbioll. 16(7): 1014-23. CrossRef PubMed
  13.  
  14. Nasu K, Narahara H. Pattern recognition via the toll-like receptor system in the human female genital tract. Mediators Inflamm. 2010; 2: 1-12 . CrossRef PubMed PubMedCentral
  15.  
  16. Lashkari BS, Shahana S, Anumba DO. Toll-like receptor 2 and 4 expression in the pregnant and non-pregnant human uterine cervix. J Reprod Immunol. 2015; 107: 43-51. CrossRef PubMed
  17.  
  18. Lim R, Barker G, Lappas M. Pellino 1 is a novel regulator of TNF and TLR signalling in human myometrial and amnion cells. J Reprod Immunol. 2018; 127: 24-35. CrossRef PubMed
  19.  
  20. Sanchez-Lopez JA, Caballero I, Montazeri M, Maslehat N, Elliott S, Fernandez-Gonzalez R, Calle A, Gutierrez-Adan A, Fazeli A. Local activation of uterine Toll-like receptor 2 and 2/6 decreases embryo implantation and affects uterine receptivity in mice. Biol Reprod, 2014; 90 (4): 1-13 . CrossRef PubMed
  21.  
  22. Chun J, Prince J. Activation of Ca2+‐dependent signaling by TLR2. J Immunol. 2006; 177 (2); 1330-37. CrossRef PubMed
  23.  
  24. Nasibian LS, Filippov IB. Modulation of rat myometrium contractile activity by peptidoglycan of Staphylococcus aureus cell wall. Fiziol Zh. 2014; 60 (5): 62-72. [Ukrainian]. CrossRef PubMed
  25.  
  26. Noble D, Borysova L, Wray S, Burdyga T. Store-operated Ca²⁺ entry and depolarization explain the anomalous behaviour of myometrial SR: effects of SERCA inhibition on electrical activity, Ca²⁺ and force. Cell Calcium. 2014; 56 (3): 188-94. CrossRef PubMed PubMedCentral
  27.  
  28. Matsuki K, Takemoto M, Suzuki Y, Yamamura H, Ohya S, Takeshima H, Imaizumi Y. Ryanodine receptor type 3 does not contribute to contractions in the mouse myometrium regardless of pregnancy. Pflugers Arch. 2017; 469 (2): 313-26. CrossRef PubMed
  29.  
  30. Gresset A, Sondek J, Harden TK. The phospholipase C isozymes and their regulation. Subcell Biochem. 2012; 58: 61-94. CrossRef PubMed PubMedCentral
  31.  
  32. Nasibyan LS, Philyppov IB. Effect of peptidoglycane of Staphylococcus Aureus cell wall on the mechanism of regulation of contractile activity of rat myometrium by adenylate cyclase system. Fiziol Zh. 2012; 62 (1): 25-33. [Ukrainian]. CrossRef  
  33. Kadamur G, Ross EM. Mammalian phospholipase C. Annu Rev Physiol. 2013; 75: 127-54. CrossRef PubMed
  34.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.