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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(4): 66-72

Peptidoglycane modulates rat myometrial contractility via Ca2+ release from SR

L.S. Nasibyan, I.B. Philyppov, Y.M. Shuba

    Bohomoletz Institute of Physiology of National Academy of Sciences of Ukraine, Kyiv, Ukraine


The aim of current trial was to investigate the SR role in the peptidoglycan elicitted alteration of myometrial contractility. For the study we used peptidoglycan from Staphylococcus aureus. In order to assess the peptidoglycan’s effect on myometrial contractility under different conditions we used the method of tensometry and took into account the average amplitude, frequency and the area under the curve of the myometrial contractions. The results of our experiments confirmed our suggestion that peptidoglycan activates myometrial contractility due to the SR depletion via IP3 receptors activation as well as the amplification of transmembrane Ca2+ influx. Under peptydoglycan the amplitude of myometrial contractions increased by nearly 7,0 ± 0.346% [n=10] compared to the control. The area under the curve increased by nearly 30,0 ± 0.256% due to both the amplitude and the duration of contractions. In our study we demonstrated that peptidoglycan makes the myometrial strip to contract even in the Ca2+-free environment throughout a few minutes. Also we demonstrated that phospholipase C blockade with U73122 doesn’t prevent entirely the stimulation of myometrial contractions by peptidoglycan. Phospholipase C blockade resulted in statistically unreliable increase in the amplitude of the peptydoglycan-stimulated myometrial contractions, their frequency decreased by 47 ± 1.05% and the area under the curve of single contraction decreased by nearly 56 ± 1.2%. But 2APB, a non selective IP3 antagonist, entirely reduced the peptidoglycan-stimulated. We suggested that peptidoglycan acts via several pathways simultaneously and Ca2+ release from SR via IP3 receptors activation is among them.

Keywords: peptidoglycane; myometrium; myometrial smooth muscle cells; myometrial contractile activity


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