THE EFFECTS OF WEAK DIRECT CURRENT ON NEURITOGENESIS IN VITRO MODEL
K.V.Yatsenko1, 2, I.V.Lushnikova1, G.G.Skibo1
- Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv, Ukraine
- Neurological clinic of Dr. Yatsenko, Kyiv, Ukraine
In this work, the effects of direct current (DC) stimulation of
nerve cells were studied in an in vitro model using short- and
long-term of dissociated hippocampal cultures. The exposure
was performed once for 4 hours using metal electrodes and a
device for generating DC. The effects of weak DC (0,25 mA)
on structural changes in cultures were assessed both in normal
conditions and in modeling the inflammatory process using
lipopolysaccharide. The area of neurites and synapse-like
structures as well as the number of glial cells were analyzed.
It was revealed that DC stimulation significantly speed up the
formation of neurites in the early period of the cultivation of
hippocampal cells. The area of neurites on average was 21,4
± 1,2% more compared to control and 25,3 ± 1,2% relatively
lipopolysaccharide for 2-5 days after DC. In long-term cultures
(10-12 days old), where the structure of neurites had the appearance
of well-developed networks, the effects of DC were
observed only with lipopolysaccharide. There was a decrease
in the disorganization of neurites and an increase in the area
of synapses by an average of 21,2 ± 1,1 %. In addition, the
number of glial cells was less by 15,6 ± 0,9 %. Thus, the
neuroprotective effects of DC can be associated with a direct
effect on neuritogenesis and the regeneration of nervous tissue.
direct current stimulation; micropolarization; hippocampal cell culture; neuritogenesis; lipopolysaccharide.
- Leffa DT, Bellaver B, Salvi AA, de Oliveira C, Caumo W, Grevet EH, Fregni F, Quincozes-Santos A, Rohde LA, Torres ILS. Transcranial direct current stimulation improves long-term memory deficits in an animal model of attention-deficit/hyperactivity disorder and modulates oxidative and inflammatory parameters. Brain Stimul. 2018; 11(4): 743-51.
- Richmond L, Wolk D, Chein J, Olson I. Transcranial direct current stimulation enhances verbal working memory training performance over time and near-transfer outcomes. J Cogn Neurosci. 2014: 26(11): 2443-54.
- Spezia Adachi LN, Caumo W, Laste G, Fernandes Medeiros L, Ripoll Rozisky J, de Souza A, Fregni F, Torres IL. Reversal of chronic stress-induced pain by transcranial direct current stimulation (tDCS) in an animal model. Brain Res. 2012; 1489: 17-26.
- Yavari F, Jamil A, Mosayebi Samani M, Vidor LP, Nitsche MA. Basic and functional effects of transcranial electrical stimulation (tES)-An introduction. Neurosci Biobehav Rev. 2018; 85: 81-92.
- Elsner B, Kugler J, Pohl M, Mehrholz J. Transcranial direct current stimulation (tDCS) for idiopathic Parkinson's disease. Cochrane Database Syst Rev. 2016; 7: CD010916.
- Grimaldi G, Argyropoulos GP, Bastian A, Cortes M, Davis NJ, Edwards DJ, Ferrucci R, Fregni F, Galea JM, Hamada M, Manto M, Miall RC, Morales-Quezada L, Pope PA, Priori A, Rothwell J, Tomlinson SP, Celnik P. Cerebellar transcranial direct current stimulation (ctDCS): a novel approach to understanding cerebellar function in health and disease. Neuroscientist. 2016; 22(1): 83-97.
- Jones KT, Stephens JA, Alam M, Bikson M, Berryhill ME. Longitudinal neurostimulation in older adults improves working memory. PLoS One. 2015; 10(4): 0121904.
- Hordacre B, Moezzi B, Ridding MC. Neuroplasticity and network connectivity of the motor cortex following stroke: A transcranial direct current stimulation study. Hum Brain Mapp. 2018; 39(8): 3326-3339.
- Martin DM, Moffa A, Nikolin S, Bennabi D, Brunoni AR, Flannery W, Haffen E, McClintock SM, Moreno ML, Padberg F, Palm U, Loo CK. Cognitive effects of transcranial direct current stimulation treatment in patients with major depressive disorder: An individual patient data meta-analysis of randomised, sham-controlled trials. Neurosci Biobehav Rev. 2018; 90: 137-145.
- Parkin BL, Bhandari M, Glen JC, Walsh V. The physiological effects of transcranial electrical stimulation do not apply to parameters commonly used in studies of cognitive neuromodulation. Neuropsychologia. 2018; S0028-3932(18): 30123-4.
- Shelyakin AM, Ponomarenko GN. Micropolarization of the brain. Theoretical and practical aspects. St.Petersburg: IPC Baltic, 2006. [Russian].
- Pelletier SJ, Lagacé M, St-Amour I, Arsenault D, Cisbani G, Chabrat A, Fecteau S, Lévesque M, Cicchetti F. The morphological and molecular changes of brain cells exposed to direct current electric field stimulation. Int J Neuropsychopharmacol. 2014; 18(5): 1-13.
- Pelletier SJ, Cicchetti F. Cellular and molecular mechanisms of action of transcranial direct current stimulation: evidence from in vitro and in vivo models. Int J Neuropsychopharmacol. 2015; 18(2): 1-16.
- Liu A, Vöröslakos M, Kronberg G, Henin S, Krause MR, Huang Y, Opitz A, Mehta A, Pack CC, Krekelberg B, Berényi A, Parra LC, Melloni L, Devinsky O, Buzsáki G. Immediate neurophysiological effects of transcranial electrical stimulation. Nat Commun. 2018; 9(1), 1-12.
- Lushnikova IV, Voronin K, Malyarevskyy PY, Smozhanyk KG, Skibo GG. Effects of oxygen-glucose deprivation of different duration on hippocampal slice cultures. Fiziol Zh. 2004; 50(2), 105-11. [Ukrainian].
Lushnikova I.V. Functional activity of hippocampal neurons after short-term oxygen-glucose deprivation in vitro. Fiziol Zh. 2008; 54(6), 58-65. [Ukrainian]
- Catorce MN, Gevorkian G. LPS-induced murine neuroinflammation model: main features and suitability for pre-clinical assessment of nutraceuticals. Curr Neuropharmacol. 2016; 14(2): 155-64.
- Lee JW, Lee YK, Yuk DY, Choi DY, Ban SB, Oh KW, Hong JT. Neuro-inflammation induced by lipopolysaccharide causes cognitive impairment through enhancement of beta-amyloid generation. J Neuroinflammation. 2008; 29(5): 37.
- Nair S, Sobotka KS, Joshi P, Gressens P, Fleiss B, Thornton C, Mallard C, Hagberg H. Lipopolysaccharide-induced alteration of mitochondrial morphology induces a metabolic shift in microglia modulating the inflammatory response in vitro and in vivo. Glia. 2019, 67(6), 1047-61.
- Maar T, Rønn L, Bock E, Berezin V, Moran J, Pasantes- Morales H, Schousboe A. Characterization of microwell cultures of dissociated brain tissue for studies of cell-cell interaction. J Neurosci Res. 1997; 47(2): 163-72.
- Yatsenko KV, Lushnikova IV, Skibo GG. Investigation of the micropolarization on neuronal cells in the modeling of the inflammatory process in vitro. Ukr Neurol J. 2018; 2(47): 69-73. [Ukrainian].
- Skibo GG, Lushnikova IV, Voronin KY, Dmitrieva O, Novikova T, Klementiev B, Vaudano E, Berezin VA, Bock E. A synthetic NCAM-derived peptide, FGL, protects hippocampal neurons from ischemic insult both in vitro and in vivo. Eur J Neurosci. 2005; 22(7): 1589-96.