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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(4): 41-49


THE EFFECTS OF WEAK DIRECT CURRENT ON NEURITOGENESIS IN VITRO MODEL

K.V.Yatsenko1, 2, I.V.Lushnikova1, G.G.Skibo1

  1. Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv, Ukraine
  2. Neurological clinic of Dr. Yatsenko, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz65.04.041

Abstract

In this work, the effects of direct current (DC) stimulation of nerve cells were studied in an in vitro model using short- and long-term of dissociated hippocampal cultures. The exposure was performed once for 4 hours using metal electrodes and a device for generating DC. The effects of weak DC (0,25 mA) on structural changes in cultures were assessed both in normal conditions and in modeling the inflammatory process using lipopolysaccharide. The area of neurites and synapse-like structures as well as the number of glial cells were analyzed. It was revealed that DC stimulation significantly speed up the formation of neurites in the early period of the cultivation of hippocampal cells. The area of neurites on average was 21,4 ± 1,2% more compared to control and 25,3 ± 1,2% relatively lipopolysaccharide for 2-5 days after DC. In long-term cultures (10-12 days old), where the structure of neurites had the appearance of well-developed networks, the effects of DC were observed only with lipopolysaccharide. There was a decrease in the disorganization of neurites and an increase in the area of synapses by an average of 21,2 ± 1,1 %. In addition, the number of glial cells was less by 15,6 ± 0,9 %. Thus, the neuroprotective effects of DC can be associated with a direct effect on neuritogenesis and the regeneration of nervous tissue.

Keywords: direct current stimulation; micropolarization; hippocampal cell culture; neuritogenesis; lipopolysaccharide.

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