THE EFFECT OF COLD STRESS ON CHANGES IN THE NUMBER OF HEMATOPOIETIC STEM AND LYMPHOID CELLS IN CENTRAL AND PERIPHERAL ORGANS IMMUNE SYSTEM
Ya M.O. Semenova, V.M. Kirik, I.S. Nikolsky, G.M. Butenko
Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz65.04.003
Abstract
The effect of acute cold stress on changes in the content of
hematopoietic stem cells (CD34iMNC-cells), lymphocytes
and granulocytes in the central and peripheral organs of the immune system of mice was studied. It was shown that 24
hours after the stress response, the number of CD34iMNC
cells in the bone marrow decreased significantly by 13% and
in the spleen by 45%. At the same time, the content of bone
marrow cells decreased by 21.9%. Also, in the blood, the
amount of reticulocytes was significantly reduced by 62.5%.
All this together proves the inhibition of the stress of bone
marrow hematopoiesis. Against this backdrop, there was a
significant increase in the number of white blood cells in
the blood of 31.3% and can be considered as a typical stress
reaction. Significant was the reduction of the content of thymus
cells by 76.1% and the spleen by 53.4% with an increase in
apoptosis among thymocytes 8.7 times, and among splenocytes
by 2 times. The number of thymocytes in the G0 / G1 phases
significantly increased, and in the phase S and G2 / M + S
significantly decreased. The obtained results indicate that the
development of acute cold stress has led to a decrease in the
content of CD34iMNC cells in the bone marrow and spleen,
as well as in the bone marrow, thymus and spleen cell division,
this is probably due to the redistribution and apoptosis of
certain cells, which should significantly change the course of
immunological processes. The results can be used to create an
experimental model for studying immunobiological processes
during short-term cold stress in order to develop methods for
improving the efficiency of regeneration of the immune system
during stress reactions.
Keywords:
cold stress; immune system; hematopoietic stem cells; lymphocytes
References
- Sapolsky RM. Stress Hormones: Good and Bad Neurobiology of Disease. Neurobiol Dis. 2000 Oct;7(5):540-2.
CrossRef
PubMed
- Bowers SL, Bilbo SD, Dhabhar FS, Nelson RJ. Stressor- specific alterations in corticosterone and immune responses in mice. Brain Behav Immun. 2008 Jan;22(1):105-13.
CrossRef
PubMed PubMedCentral
- Nikolsky IS, NikolskayaVV, Demchenko DL, Taranukha LI, Semenova YA, Serebrovska TV. Effects of multipotent stromal cell transplantation on mice immune system under conditions of its regeneration. Fiziol Zh. 2018; 64(4): 3-11. [Ukrainian].
CrossRef
- Zieziulewicz TJ, Mondal TK, Gao D, Lawrence DA. Stress-induced effects, which inhibit host defenses, alter leukocyte trafficking. Cell Stress Chaperones. 2013 May;18(3):279-91.
CrossRef
PubMed PubMedCentral
- Park S, Lee MS, Jung S, Lee S, Kwon O, Kreuter MH, Perrinjaquet-Moccetti T, Min B, Yun SH, Kim Y. Echinacea purpurea Protects Against Restraint Stress-Induced Immunosuppression in BALB/c Mice. J Med Food. 2018 Mar;21(3):261-8.
CrossRef
PubMed
- Hu GZ, Yang SJ, Hu WX, Wen Z4, He D, Zeng LF, Xiang Q, Wu XM, Zhou WY, Zhu QX. Effect of cold stress on immunity in rats. Exp Ther Med. 2016 Jan;11(1):33-42.
CrossRef
PubMed PubMedCentral
- Sun S, Zhou J. Molecular mechanisms underlying stress response and adaptation. Thorac Cancer. 2018 Feb;9(2):218-27.
CrossRef
PubMed PubMedCentral
- Stelzer I, Kröpfl JM, Fuchs R, Pekovits K, Mangge H, Raggam RB, Gruber HJ, Prüller F, Hofmann P, Truschnig- Wilders M, Obermayer-Pietsch B, Haushofer AC, Kessler HH, Mächler P. Ultra-endurance exercise induces stress and inflammation and affects circulating hematopoietic progenitor cell function. Scand J Med Sci Sports. 2015 Oct;25(5):e442-50.
CrossRef
PubMed
- Melo RCC, Ferro KPV, Duarte ADSS, Olalla Saad ST. CXCR7 participates in CXCL12-mediated migration and homing of leukemic and normal hematopoietic cells. Stem Cell Res Ther. 2018 Feb 12;9(1):34.
CrossRef
PubMed PubMedCentral
- Ogle ME, Olingy CE, Awojoodu AO, Das A, Ortiz RA, Cheung HY, Botchwey EA. Sphingosine-1-Phosphate Receptor-3 Supports Hematopoietic Stem and Progenitor Cell Residence Within the Bone Marrow Niche. Stem Cells. 2017 Apr;35(4):1040-52.
CrossRef
PubMed PubMedCentral
- Lisianyĭ MI. Mesenchymal stem cells and their im munological properties. Fiziol Zh. 2013;59(3):126-34. [Ukrainian].
CrossRef
PubMed
- Zhou Y, Li H, Siddiqui N, Caudle Y, Zhang H, Elgazzar M, Yin D. Hematopoietic stem progenitor cells prevent chronic stress-induced lymphocyte apoptosis. J Neuroimmunol. 2017 Aug 15;309:72-7.
CrossRef
PubMed PubMedCentral
- Costa MHG, de Soure AM, Cabral JMS, Ferreira FC, da Silva CL. Hematopoietic Niche - Exploring Biomimetic Cues to Improve the Functionality of Hematopoietic Stem/Progenitor Cells. Biotechnol J. 2018 Feb;13(2).
CrossRef
PubMed
- Sowers JR, Raj RP, Hershman JM, Carlson HE, McCallum RW. The effect of stressful diagnostic studies and surgery on anterior pituitary hormone release in man. Acta Endocrinol (Copenh). 1977 Sep;86(1):25-32.
CrossRef
PubMed
- Schmelzer E, Foka HG, Thompson RL, Luca A, Gridelli B, Gerlach JC. Response of Human Fetal Liver Progenitor Cell Types to Temperature and pH Stresses In Vitro. Rejuvenation Res. 2018 Jun;21(3):257-69.
CrossRef
PubMed
- Sim HJ, Kook SH, Yun CY, Bhattarai G, Cho ES, Lee JC. Brief Report: Consecutive Alendronate Administration- Mediated Inhibition of Osteoclasts Improves Long-Term Engraftment Potential and Stress Resistance of HSCs. Stem Cells. 2016 Oct;34(10):2601-7.
CrossRef
PubMed
- Bible LE, Pasupuleti LV, Gore AV, Sifri ZC, Kannan KB, Mohr AM.Chronic restraint stress after injury and shock is associated with persistent anemia despite prolonged elevation in erythropoietin levels. J Trauma Acute Care Surg. 2015 Jul;79(1):91-6; discussion 96-7.
- Zabelinskii SA, Chebotareva MA, Tavrovskaya TV, Skverchinskaya EA, Shukolyukova EP, Maslova MN, Krivchenko AI. Effect of stress actions on some hematologic and biochemical parameters of rat blood and on energetic intermolecular interactions in lipid extract under effect of light radiation. J Evolutionary Biochem and Physiol. 2012; 48(6):548-56. [Russian].
CrossRef
- Kim TS, Hanak M, Trampont PC, Braciale TJ. Stress-1 conventional dendritic cells. J Clin Invest. 2015 Oct 1;125(10):3965-80.
CrossRef
PubMed PubMedCentral
- Boehm T, Bleul CC. Thymus-homing precursors and the thymic microenvironment. Trends Immunol. 2006 Oct;27(10):477-84.
CrossRef
PubMed
- Chebotarev VF. Endocrine regulation of immunogenesis. Zdorov'ya. 1979:160. [Ukrainian].
- Dudakov JA, Goldberg GL, Reiseger JJ, Vlahos K, Chidgey AP, Boyd RL. Sex steroid ablation enhances hematopoietic recovery following cytotoxic antineoplastic therapy in aged mice. J Immunol. 2009 Dec 1;183(11):7084-94.
CrossRef
PubMed
- Stavinskaya OA. Change of activity apoptosis of lymphocytes at short-term general cooling of the person. interrelation with the level of the immune background. J Ural Med Acad Sci. 2018; 15(2):309-15. [Russian].
CrossRef
- Wu C, Espinoza DA, Koelle SJ, Potter EL, Lu R, Li B, Yang D, Fan X, Donahue RE, Roederer M, Dunbar CE. Geographic clonal tracking in macaques provides insights into HSPC migration and differentiation. J Exp Med. 2018 Jan 2;215(1):217-32.
CrossRef
PubMed PubMedCentral
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