Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(3): 28-33


O.M. Voloshchuk, G.P. Kopylchuk

    Yuriy Fedkovych Chernivtsi National University, Ukraine


Indicators of the water-salt balance in rats under the conditions of acetaminophen-induced injury and alimentary protein deficiency were studied in the research. Blood levels of Na+, K+, Mg2+, Cl- ions were measured by method of spectrophometry using reagent sets (Human, Germany). Aldosterone level was determined by enzyme-linked immunosorbent assay method using Aldosterone ELISA kit. It is established that significant disturbances of water-salt balance were observed in a group of animals with the toxic acetaminophen-induced injury against the background of alimentary protein deficiency. In animals of the combined pathology group a hypernatremia (Na+ content increased by 25%), hyperchloremia (Cl- content increased by 30%) along with hypokalemia (K+ content decreased by 20%) and hypomagnesemia (Mg2+ content decreased by 1,8 times) was revealed, indicating the formation of ion imbalance. A simultaneous increase in aldosterone level in 2,2 times and plasma hyperosmoralily indicates the formation of the state of hyperhydration. The obtained results can be used to develop a strategy for correction of water-salt balance disturbances in conditions of acetaminophen-induced toxic injury on the background of alimentary protein deficiency.

Keywords: alimentary protein deprivation; acetaminophen; toxic damage; electrolytes; osmolarity; aldosterone.


  1. Wu G. Dietary protein intake and human health. Food Funct. 2016; 7(3): 1251-65. CrossRef PubMed
  3. Smith SE, Figley SA, Schreyer DJ, Paterson PG. Proteinenergy malnutrition developing after global brain ischemia induces an atypical acute-phase response and hinders expression of GAP-43. PLoS One. 2014; 9(9): e107570. CrossRef PubMed PubMedCentral
  5. Voloshchuk ON, Kopylchuk GP. The ratio of ubiqiunon redox forms in the liver mitochondric under toxic hepatitis induced on the background of alimentary protein deficiency. Vopr Pitan. 2015; 84 (5): 82-7. [Russian].
  7. Hong JH, Park S, Shcheynikov N, Muallem S. Mechanism and synergism in epithelial fluid and electrolyte secretion. Pflug. Arch. 2014; 466(8): 1487-99. CrossRef PubMed PubMedCentral
  9. El-Sharkawy AM, Sahota O, Maughan RJ, Lobo DN. The pathophysiology of fluid and electrolyte balance in the older adult surgical patient. Clin Nutrition. 2014; 3: 6e13. CrossRef PubMed
  11. Dhondup T, Qian Q. Electrolyte and acid - base disorders in chronic kidney disease and end-stage kidney failure. Blood Purif. 2017; 43: 179-88. CrossRef PubMed
  13. Piasecki R, Michalak A, Cichoż-Lach H. The role of electrical bioimpedance in the evaluation of the hydration status of patients with liver cirrhosis. Pol Merkur Lekarski. 2018; 45(267): 122-5.
  15. Nishikawa H, Yoh K, Enomoto H, Ishii N, Iwata Y, Nakano C, Takata R, Nishimura T, Aizawa N, Sakai Y, Ikeda N, Hasegawa K, Takashima T, Iijima H, Nishiguchi S. Extracellular water to total body water ratio in viral liver diseases: a study using bioimpedance analysis. Nutrients. 2018; 10(8): 1072. CrossRef PubMed PubMedCentral
  17. Kopylchuk GP, Voloshchuk ON, Buchkovskaia IM, Davydenko IS. Morphological characteristic of rats' kidneys under the conditions of acetaminophen-induced nephrotoxicity against the background alimentary deprivation of protein. Morphologia. 2015;9(3): 28-30. [Ukrainian].
  19. Voloshchuk OM, Kopylchuk GP, Mishyna YI. Activity of the mitochondrial isoenzymes of endogenous aldehydes catabolism under the conditions of acetaminopheninduced hepatitis. Ukr Biochem J. 2018; 90 (1):42-7. [Ukrainian]. CrossRef  
  20. Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents: final report of the american institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993; (5):1939-51. CrossRef PubMed
  22. Heavens KR, Kenefick RW, Caruso EM, Spitz MG, Cheuvront SN. Validation of equations used to predict plasma osmolality in a healthy adult cohort. Am J Clin Nutr. 2014; 100: 1252-6. CrossRef PubMed
  24. Halushko OA, Bolyuk MV. Correction of sodium exchange disorders in intensive care patients: old methods and modern approaches. Emergency Med. 2018; 2: 40-7. [Ukrainian]. CrossRef  
  25. Farquhar WB, Edwards DG, Jurkovitz CT, Weintraub WS. Dietary sodium and health: more than just blood pressure. J Am Coll Cardiol. 2015; 65(10): 1042-50. CrossRef PubMed PubMedCentral
  27. Viera NJ, Wouk N. Potassium disorders: hypokalemia and hyperkalemia. Am Fam Physician. 2015; 92(6): 487-95.
  29. Jain N, Kotla S, Little BB, Weideman RA, Brilakis ES, Reilly RF, Banerjee S. Predictors of hyperkalemia and death in patients with cardiac and renal disease. Am J Cardiol. 2012; 109: 1510-3. CrossRef PubMed
  31. Attia ZI, DeSimone CV, Dillon JJ, Sair Y, Somers VK, Dugan JL, Bruce CJ, Ackerman MJ, Asirvatham SJ, Striemer BL, Bukartyk J, Scott CG, Bennet KE, Ladewig DJ, Gilles EJ, Sadot D, Geva AB, Friedman PA. Novel bloodless potassium determination using a signal‐processed single‐ lead ECG. J Am Heart Assoc. 2016; 5: e002746. CrossRef PubMed PubMedCentral
  33. Litovka IG, Berezovskii VA Effect of magnesium on bone tissue remodeling. Fiziol Zh. 2018; 64(3): 91-9. [Ukrainian]. CrossRef  
  34. Hashemi R, Majidi A, Tabatabaey A, Mahmoudi S. Calculation of plasma chloride levels using blood gas measurements. J Emergency Practice and Trauma. 2017; 3(2): 42-5. CrossRef  
  35. Palmer LG, Frindt G. Regulation of epithelial Na channels by aldosterone. Kitasato Med J. 2016; 46: 1-7.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.