CHARACTERISTICS OF WATER-SALT BALANCE IN PROTEIN-DEFICIENCY RATS WITH ACETAMINOPHEN-INDUCED TOXIC INJURY
O.M. Voloshchuk, G.P. Kopylchuk
Yuriy Fedkovych Chernivtsi National University, Ukraine
DOI: https://doi.org/10.15407/fz65.03.028
Abstract
Indicators of the water-salt balance in rats under the conditions
of acetaminophen-induced injury and alimentary protein deficiency
were studied in the research. Blood levels of Na+, K+,
Mg2+, Cl-
ions were measured by method of spectrophometry
using reagent sets (Human, Germany). Aldosterone level was
determined by enzyme-linked immunosorbent assay method
using Aldosterone ELISA kit. It is established that significant
disturbances of water-salt balance were observed in a group of
animals with the toxic acetaminophen-induced injury against
the background of alimentary protein deficiency. In animals of
the combined pathology group a hypernatremia (Na+ content
increased by 25%), hyperchloremia (Cl-
content increased by
30%) along with hypokalemia (K+ content decreased by 20%)
and hypomagnesemia (Mg2+ content decreased by 1,8 times)
was revealed, indicating the formation of ion imbalance. A
simultaneous increase in aldosterone level in 2,2 times and
plasma hyperosmoralily indicates the formation of the state of
hyperhydration. The obtained results can be used to develop
a strategy for correction of water-salt balance disturbances
in conditions of acetaminophen-induced toxic injury on the
background of alimentary protein deficiency.
Keywords:
alimentary protein deprivation; acetaminophen; toxic damage; electrolytes; osmolarity; aldosterone.
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