The regulatory mechanisms of oxygen regime in muscle cell by O2 free diffusion from the microcirculatory to tissue and intracellular diffusion O2 transport to the mitochondria were considered. Diffusion O2 pathway to tissue is divided on 2 parts the first - convective intravessel O2 flow and its leak from microvessels to tissue, the second - intracellular diffusion O2 to mitochondria. The analysis of mathematical modeling and literature data showed: in skeletal muscle there is a functional nutritional network, which changes its structure and properties depending on blood flow velocity and the density of microcirculation in skeletal muscle (1), the self-regulation of the diffusion O2 transport from blood to tissue counteracts hyperoxia at rest and hypoxia under workload (2). Tissue oxygen regime under circulatory, gemic and hypoxic hypoxia was studied. Mean value of cell O2 uptake (VO2) shown to be preserved at hypoxia if mitochondrial oxidative power is distributed according to VO2 gradients. Uneven intracellular distribution of mitochondria reduces the diffusion barriers inside the cell and regulates cellular oxygen regime by weakening tissue hypoxia and counteracting the suppression of tissue respiration,