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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(2): 12-21


N.O. Volkova, M.S. Yukhta, A.M. Goltsev

    Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine


The study investigated the effect of growth and differentiation factors on cryopreserved mesenchymal stromal cells from bone marrow, adipose and cartilage tissues. It was shown that the addition of fibroblast growth factor (20 ng / ml) to the culture medium resulted in a 2.6 and 2.1 fold increasing in the proliferative potential of cryopreserved mesenchymal stromal cells (CrMSC) from bone marrow and adipose tissue. The use of transforming growth factor β in concentration 10 ng/ml increased the synthesis of type II collagen by 7.2 and 6.1 times and glycosaminoglycans in 5.1 and 2.6 times, respectively, for CrMSCs from bone marrow and adipose tissue on the 12th day of culturing. Proliferative activity, the synthesis of type II collagen and glycosaminoglycans of CrMSCs from cartilaginous tissue did not undergo changes under the influence of the investigated factors, but they were characterized by a relatively greater activity of synthetic processes compare to CrMSCs from bone marrow and adipose tissue. The obtained data can be used to substantiate and develop a technique for the application of CrMSCs in clinical practice to treat the damages of musculoskeletal system.

Keywords: mesenchymal stromal cells; growth factors; proliferation; collagen; glycosaminoglycans.


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