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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(1): 57-65


V.V. Lomako, O.V. Shylo, I.F. Kovalenko

    Institute for problems of cryobiology and cryomedicine of the NAS of Ukraine, Kharkov, Ukraine


Summary. The dynamics of the transformation of erythrocytes in young (6-7 months) and old (18-20 months) rats after the whole body cryostimulation (WBC) was studied by the method of low-angle light scattering. The specific ratios of discocytes, reversibly and irreversibly modified forms of the erythrocytes were used for their transformation indices calculation. The WBC (at a temperature -120 ºC, the duration was 90 sec and 3 sessions were conducted with an interval of 24 hs) was carried out in an experimental cryochamber designed for cooling of small laboratory animals. The number of discocytes, especially their flattened shapes (highly resistant to the factors of hemolysis) was higher (by 17.3 of normocytes and 43.5% of flattened forms) in the blood of control old rats. In the contrary, the number of altered forms of the erythrocytes (stomatocytes and spherocytes) was lower in the blood of old (by 38.4 and 23.5%, respectively) than in young rats. The indices of erythrocytes transformation were lower in old rats; especially of the index of reversibility transformation (more than 7 times). The direction and manifestation of changes of the erythrocyte population composition and transformation after the WBC were depended on the age, number of cryoexposures (1, 2 or 3) and the observation time (after a day or week). The WBC led to the higher heterogeneity of the erythrocytes population: the parts of reversibly and irreversibly altered cell forms increased in the blood. The indices of erythrocytes transformation decreased almost at all the stages of the experiment both in young and old rats.

Keywords: erythrocytes; whole body cryostimulation; age; rats


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