Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(1): 50-56


KINETICS OF SINGLE CONTRACTIONS INHIBITION UNDER ISCHEMIA-REPERFUSION AS AN INDICATOR OF M. GASTROCNEMIUS FUNCTIONAL STATE IN RATS

D.M. Nozdrenko, V.M. Soroka, O.M.Khoma

    Taras Shevchenko National University of Kyiv, ESC “Institute of Biology and Medicine”, Ukraine
DOI: https://doi.org/10.15407/fz65.01.050


Abstract

The degradation of strength and lengths of isotonic twitch contractions of rat gastrocnemius muscle under ischemiareperfusion conditions was described in the article. Was demonstrated that strength and lengths recession directly dependent on ischemia and subsequent reperfusion duration. It was found that in the selected conditions detectable muscle contraction reaches zero level after 90-100 minutes of the ischemia. In the reperfusion phase partial contraction restoration was detected. Recovery level depended on preliminary ischemia duration. Maximum recovery level was observed after 4 (2+2) hours of ischemia-reperfusion, strengths restored up to 72±3,2% and lengths reached 7±1,3% of nonischemic control. In the case of 3+3 and 4+4 hours of ischemia-reperfusion function regenerated up to 42±4,1% and 12±2,3% of strengths control accordingly, lengths stayed on zero level in both situations. Contraction properties in reperfusion period recovered to some steady state level that probably allows us to determine muscle damage in ischemia stage. Simples, utility and possibility of noninvasive use of this method of muscle function evaluation can be useful in clinical practice.

Keywords: ischemia-reperfusion; skeletal muscle; isotonic twitch; strength; length.

References

  1. Wang WZ, Baynosa RC, Zamboni WA. Therapeutic interventions against reperfusion injury in skeletal muscle. J Surg Res. 2011;171(1):175-82. CrossRef PubMed
  2.  
  3. Turóczi Z, Arányi P, Lukáts Á, Garbaisz D, Lotz G, Harsányi L, Szijártó A. Muscle fiber viability, a novel method for the fast detection of ischemic muscle injury in rats. PLoS One. 2014;9(1):e84783. CrossRef PubMed PubMedCentral
  4.  
  5. Nozdrenko DM, Bogutska KI, Prylutskyy YuI, Korolovych VF, Evstigneev MP, Ritter U, Scharff P. Impact of C60 fullerene on the dynamics of force-speed changes in soleus muscle of rat at ischemia-reperfusion injury. Fiziol Zh. 2015; 61(2):48-59.[Ukrainian]. CrossRef PubMed
  6.  
  7. Khoma OM, Zavodovs'kyĭ DA, Nozdrenko DN, Dolhopolov OV, Miroshnychenko MS, Motuziuk OP. Dynamics of ischemic skeletal soleus muscle contraction in rats. Fiziol Zh. 2014;60(1):34-40. [Ukrainian]. CrossRef PubMed
  8.  
  9. Nozdrenko DN, Shut AN, Prylutskyy YI. The possible molecular mechanism of the nonlinearity muscle contraction and its experimental substantiation. Biopolymers and Cell. 2005;21(1):80-83. [Ukrainian]. CrossRef  
  10. Loerakker S, Oomens CW, Manders E, Schakel T, Bader DL, Baaijens FP, Nicolay K, Strijkers GJ. Ischemiareperfusion injury in rat skeletal muscle assessed with T2-weighted and dynamic contrast-enhanced MRI. Magn Reson Med. 2011; 66(2):528-37. CrossRef PubMed
  11.  
  12. Nozdrenko DN, Bogutska KI. About molecular mechanisms of fiber muscle contraction at transition to new equilibrium state: Analysis of experimental data using three-componential electrical stimulating signal. Biopolymers and Cell. 2005; 21(3):285-286. [Ukrainian]. CrossRef  
  13. Erkut B., Özyazıcıoğlu A., Karapolat B.S., Koçoğulları C.U., Keles S., Ateş A., Gundogdu C, Kocak H. Effects of ascorbic Acid, alpha-tocopherol and allopurinol on ischemia-reperfusion injury in rabbit skeletal muscle: an experimental study. Drug Target Insights. 2007;2:249-258. CrossRef PubMed PubMedCentral
  14.  
  15. Rácz IB, Illyés G, Sarkadi L, Hamar J. The functional and morphological damage of ischemic reperfused skeletal muscle. J.Eur Surg Res. 1997;29(4):254-263. CrossRef PubMed
  16.  
  17. Bortolotto SK, Morrison WA, Messina A. The role of mast cells and fibre type in ischaemia reperfusion injury of murine skeletal muscles. J. Inflamm. (Lond). 2004;1(1):2. CrossRef PubMed PubMedCentral
  18.  
  19. Zay SY, Zavodovskyi DA, Bogutska KI, Nozdrenko DN, Prylutskyy YI. Prospects of C60 fullerene application as a mean of prevention and correction of ischemicreperfusion injury in the skeletal muscle tissue. Fiziol Zh. 2016;62(3): 66-77. [Ukrainian]. CrossRef PubMed
  20.  
  21. Nocella M, Colombini B, Benelli G, Cecchi G, Bagni MA and Bruton J. Force decline during fatigue is due to both a decrease in the force per individual crossbridge and the number of cross-bridges. J. Physiol, 2011;589:3371 3381. CrossRef PubMed PubMedCentral
  22.  
  23. Allen DG, Lamb GD And Westerblad H. Skeletal Muscle Fatigue: Cellular Mechanisms. Physiol Rev January 2008;88(1):287-332. CrossRef PubMed
  24.  
  25. Carvalho AJ, McKee NH, Green HJ. Metabolic and contractile responses of fast and slow twitch rat skeletal muscles to ischemia and reperfusion. Plast Reconstr Surg. 1997;99(1):163-71. CrossRef PubMed
  26.  
  27. Racz I, Illyés G, Sarkadi L, Hamar J. The functional and morphological damage of ischemic reperfused skeletal muscle. Rácz J.Eur Surg Res. 1997; 29(4):254-63. CrossRef PubMed
  28.  
  29. Nozdrenko DM, Zavodovskyi DO, Matvienko TYu, Zay SYu, Bogutska KI, Prylutskyy YuI, Ritter U and Scharff P. C60 fullerene as promising therapeutic agent for the prevention and correction of skeletal muscle functioning at ischemic injury. Nanoscale Res Lett. 2017;12(1):115. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.