KINETICS OF SINGLE CONTRACTIONS INHIBITION UNDER ISCHEMIA-REPERFUSION AS AN INDICATOR OF M. GASTROCNEMIUS FUNCTIONAL STATE IN RATS
D.M. Nozdrenko, V.M. Soroka, O.M.Khoma
Taras Shevchenko National University of Kyiv, ESC “Institute of Biology and Medicine”, Ukraine
DOI: https://doi.org/10.15407/fz65.01.050
Abstract
The degradation of strength and lengths of isotonic twitch
contractions of rat gastrocnemius muscle under ischemiareperfusion
conditions was described in the article. Was demonstrated
that strength and lengths recession directly dependent
on ischemia and subsequent reperfusion duration. It was found
that in the selected conditions detectable muscle contraction
reaches zero level after 90-100 minutes of the ischemia. In the
reperfusion phase partial contraction restoration was detected.
Recovery level depended on preliminary ischemia duration.
Maximum recovery level was observed after 4 (2+2) hours
of ischemia-reperfusion, strengths restored up to 72±3,2%
and lengths reached 7±1,3% of nonischemic control. In the
case of 3+3 and 4+4 hours of ischemia-reperfusion function
regenerated up to 42±4,1% and 12±2,3% of strengths control
accordingly, lengths stayed on zero level in both situations.
Contraction properties in reperfusion period recovered to some
steady state level that probably allows us to determine muscle
damage in ischemia stage. Simples, utility and possibility of
noninvasive use of this method of muscle function evaluation
can be useful in clinical practice.
Keywords:
ischemia-reperfusion; skeletal muscle; isotonic twitch; strength; length.
References
- Wang WZ, Baynosa RC, Zamboni WA. Therapeutic interventions against reperfusion injury in skeletal muscle. J Surg Res. 2011;171(1):175-82.
CrossRef
PubMed
- Turóczi Z, Arányi P, Lukáts Á, Garbaisz D, Lotz G, Harsányi L, Szijártó A. Muscle fiber viability, a novel method for the fast detection of ischemic muscle injury in rats. PLoS One. 2014;9(1):e84783.
CrossRef
PubMed PubMedCentral
- Nozdrenko DM, Bogutska KI, Prylutskyy YuI, Korolovych VF, Evstigneev MP, Ritter U, Scharff P. Impact of C60 fullerene on the dynamics of force-speed changes in soleus muscle of rat at ischemia-reperfusion injury. Fiziol Zh. 2015; 61(2):48-59.[Ukrainian].
CrossRef
PubMed
- Khoma OM, Zavodovs'kyĭ DA, Nozdrenko DN, Dolhopolov OV, Miroshnychenko MS, Motuziuk OP. Dynamics of ischemic skeletal soleus muscle contraction in rats. Fiziol Zh. 2014;60(1):34-40. [Ukrainian].
CrossRef
PubMed
- Nozdrenko DN, Shut AN, Prylutskyy YI. The possible molecular mechanism of the nonlinearity muscle contraction and its experimental substantiation. Biopolymers and Cell. 2005;21(1):80-83. [Ukrainian].
CrossRef
- Loerakker S, Oomens CW, Manders E, Schakel T, Bader DL, Baaijens FP, Nicolay K, Strijkers GJ. Ischemiareperfusion injury in rat skeletal muscle assessed with T2-weighted and dynamic contrast-enhanced MRI. Magn Reson Med. 2011; 66(2):528-37.
CrossRef
PubMed
- Nozdrenko DN, Bogutska KI. About molecular mechanisms of fiber muscle contraction at transition to new equilibrium state: Analysis of experimental data using three-componential electrical stimulating signal. Biopolymers and Cell. 2005; 21(3):285-286. [Ukrainian].
CrossRef
- Erkut B., Özyazıcıoğlu A., Karapolat B.S., Koçoğulları C.U., Keles S., Ateş A., Gundogdu C, Kocak H. Effects of ascorbic Acid, alpha-tocopherol and allopurinol on ischemia-reperfusion injury in rabbit skeletal muscle: an experimental study. Drug Target Insights. 2007;2:249-258.
CrossRef
PubMed PubMedCentral
- Rácz IB, Illyés G, Sarkadi L, Hamar J. The functional and morphological damage of ischemic reperfused skeletal muscle. J.Eur Surg Res. 1997;29(4):254-263.
CrossRef
PubMed
- Bortolotto SK, Morrison WA, Messina A. The role of mast cells and fibre type in ischaemia reperfusion injury of murine skeletal muscles. J. Inflamm. (Lond). 2004;1(1):2.
CrossRef
PubMed PubMedCentral
- Zay SY, Zavodovskyi DA, Bogutska KI, Nozdrenko DN, Prylutskyy YI. Prospects of C60 fullerene application as a mean of prevention and correction of ischemicreperfusion injury in the skeletal muscle tissue. Fiziol Zh. 2016;62(3): 66-77. [Ukrainian].
CrossRef
PubMed
- Nocella M, Colombini B, Benelli G, Cecchi G, Bagni MA and Bruton J. Force decline during fatigue is due to both a decrease in the force per individual crossbridge and the number of cross-bridges. J. Physiol, 2011;589:3371 3381.
CrossRef
PubMed PubMedCentral
- Allen DG, Lamb GD And Westerblad H. Skeletal Muscle Fatigue: Cellular Mechanisms. Physiol Rev January 2008;88(1):287-332.
CrossRef
PubMed
- Carvalho AJ, McKee NH, Green HJ. Metabolic and contractile responses of fast and slow twitch rat skeletal muscles to ischemia and reperfusion. Plast Reconstr Surg. 1997;99(1):163-71.
CrossRef
PubMed
- Racz I, Illyés G, Sarkadi L, Hamar J. The functional and morphological damage of ischemic reperfused skeletal muscle. Rácz J.Eur Surg Res. 1997; 29(4):254-63.
CrossRef
PubMed
- Nozdrenko DM, Zavodovskyi DO, Matvienko TYu, Zay SYu, Bogutska KI, Prylutskyy YuI, Ritter U and Scharff P. C60 fullerene as promising therapeutic agent for the prevention and correction of skeletal muscle functioning at ischemic injury. Nanoscale Res Lett. 2017;12(1):115.
CrossRef
PubMed PubMedCentral
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