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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(1): 15-19


INHIBITION OF BRAIN ASICS AFFECTS HIPPOCAMPAL THETA-RHYTHM AND OPENFIELD BEHAVIOR IN RATS

M.P. Fedoriuk1, A.O. Cherninskyi1, O.P. Maximyuk1, D.S. Isaev1, R.I. Bogovyk1, A.V. Venhreniuk1, O.M. Boyko2, O.O. Krishtal1

  1. Bogomoletz Institute of Physiology, Kyiv, Ukraine
  2. Taras Shevchenko National University of Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz65.01.015

Abstract

The acid-sensing ion channels (ASICs) are present on the cellular membranes of virtually all mammalian central neurons, but their physiological role still remains to be discovered. Here, we described the effect of novel orthosteric ASIC antagonist, compound 5b, on rat’s hippocampal integral electrical signal exposed to the open field behavior test. The analysis of experimental rat’s EEGs revealed that 5b-administered animals demonstrate substantial decrease in theta rhythm frequency. Lowering the theta rhythm frequency is specific for anxiolytics - pharmacological agents (e.g. benzodiazepines, barbiturates, etc.), that are used in medicine for anxiety-related disorders treatment. Under 5b, the animal behavior was also significantly altered from that in control: in rats with suppressed ASIC channels, the locomotor function was reduced similarly to the effect of a large dose of anxiolytics. Using small-molecule orthosteric ASIC antagonist, we characterized for the first time the effect of pharmacological blockade of these channels on electrical activity of hippocampus and behavioral reactions of rats. Our data support the results of previous studies on the participation of ASIC channels in regulation of behavioral reactions and negative emotional states such as anxiety and depression.

Keywords: ASIC; EEG; open-field; compound 5b.

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