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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2019; 65(1): 3-14


CORRECTION OF BONE MARROW SYNDROME IN IRRADIATED MICE BY TRANSPLANTATION OF HEMATOPOIETIC STEM CELLS PRE-INCUBATED WITH THYMIC MULTIPOTENT STROMAL CELLS

K.I. Nikolska, G.M.Butenko

    State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv,Ukraine
DOI: https://doi.org/10.15407/fz65.01.003

Abstract

The transplantation of hematopoietic stem (HSC) and progenitors from bone marrow cells (BMC) and fetal liver cells (FLC) was positively noted on the immune system parameters of lethally irradiated mice. The development of bone marrow syndrome was inhibited: the thymic mass and cellularity of thymus and bone marrow increased substantially, the ability of bone marrow stromal cells to form fibroblast colonies increased. The natural cytotoxic and proliferative activity of lymphocytes significantly increased. Pre-incubation of BMC with thymic multipotent stromal cells (MSC) resulted in a significant increase in immunological parameters. There was observed a 35 % increase of in the spleen cell count, a 20 % increase in the number of lymphocytes in peripheral blood, stimulation by 17 % of natural cytotoxicity, 47 % proliferative activity of T lymphocytes, 2.8 times synthesis of α/β- and 1.7- fold γ-interferons, a 50 % increase in the amount of spontaneous ФНПα and activation of antibody formation in 2.8 times. FLCs induced by thymic MSCs showed significant activity in some parameters. There was an increase of 10.3 times in mass and 6.8 times in thymic cell count, 52 % in lymphocytes in peripheral blood, stimulation by 69 % of natural cytotoxicity, 1.8 times synthesis of α/β- and 2.9 times in γ-interferons, activation of antibody formation in 2,7 times. The effect of BMC and FLC sometimes differed quantitatively, but was always unidirectional. The results can be used to develop methods for increasing the regenerative and radioprotective activity of HSC transplants.

Keywords: radiation immunodeficiency, regeneration, hematopoietic stem cells, multipotent stromal cells, intercellular interaction, transplantation.

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