Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(6): 91-98


STRUCTURAL AND ELECTROPHYSIOLOGICAL CHANGES IN SCIATIC NERVE AFTER INTRACEREBRAL HEMORRHAGE IN RATS

І.M. Dovgan

    Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.06.091

Abstract

The structural and electrophysiological changes of the peripheral nerve on 10th, 30th and 90th day after unilateral stroke (posttraumatic intracerebral hematoma in the right hemisphere) in the Wistar rats were studied. Electrophysiological and morphological values of the left and right nerves in rats after stroke with/without pharmacological correction were compared. The electrophysiological study has shown a statistically significant decrease in conduction velocity of the sciatic nerve on 30th and 90th day of stroke. The early structural changes in the nerve on the 10th day of stroke (deformation and decrease the thickness and density of nerve fibers) were registered in morphological study. The progress of neurodegeneration on the 30th and 90th days (loss of nerve fibers and the appearance of free Schwann cells) was also shown. The greater level of degenerative changes in the left sciatic nerve in comparison with the right nerve was verified through the morphometric method. The sciatic nerve conduction velocity on 90th day after the treatment (quercetin and phosphatidylcholine) was significantly greater compared with 30th and 90th days without treatment. The increase in conduction velocity may indicate on the adaptive and restorative changes in the nerve induced by treatment. The results of histological studies have shown that a unilateral stroke can induce bilateral neurodegeneration in the sciatic nerve, and the proposed pharmacological treatment may result in restore the conduction velocity in the nerve in the long-term period.

Keywords: intracerebral hemorrhage; neurodegeneration; sciatic nerve; nerve conduction velocity; morphometry

References

  1. Hatem SM, Hatem, SM, Saussez G, della Faille M, Prist V, Zhang X, Dispa D, Bleyenheuft Y. Rehabilitation of motor function after stroke: a multiple systematic review focused on techniques to stimulate upper extremity recovery. Front Hum Neurosci. 2016; 10: 442. CrossRef PubMed PubMedCentral
  2.  
  3. Cheng CY, Hsu CY, Huang YC, Tsai YH, Hsu HT, Yang WH, Lin HC, Wang TC, Cheng WC, Yang JT, Lee TC, Lee MH. Motor outcome of deep intracerebral haemorrhage in diffusion tensor imaging: comparison of data from different locations along the corticospinal tract. Neurol Res. 2015; 37: 774-81. CrossRef PubMed
  4.  
  5. Hunkar R, Balci K. Entrapment neuropathies in chronic stroke patients. J Clin Neurophysiol. 2012; 29(1): 96-100. CrossRef PubMed
  6.  
  7. Uğurlu FG, Tiftik T, Kara M, Türkkan C, Ersöz M, Akkuş S, Özçakar L. Ultrasonographic evaluation of the median and sciatic nerves in hemiplegic patients after stroke. Am J Phys Med Rehabil. 2015; 94(6): 429-35. CrossRef PubMed
  8.  
  9. Chroni E, Argyriou AA, Katsoulas G, Polychronopoulos P. Ulnar F wave generation assessed within 3 days after the onset of stroke in patients with relatively preserved level of consciousness. Clin Neurol Neurosurg. 2007; 109(1): 27-31. CrossRef PubMed
  10.  
  11. Pollock M, Nukada H, Allpress S, Calder C, Mackinnon M. Peripheral nerve morphometry in stroke patients. J Neurol Sci. 1984; 65(3): 341-52. CrossRef  
  12. Weissmiller AM, Wu C. Current advances in using neurotrophic factors to treat neurodegenerative disorders. Translational Neurodegeneration, 2012; 1: 14. CrossRef PubMed PubMedCentral
  13.  
  14. Ghosh A, Sarkar S, Mandal AK, Das N. Neuroprotective role of nanoencapsulated quercetin in combating ischemia-reperfusion induced neuronal damage in young and aged rats. PLoS ONE. 2013; 8(4): e57735. CrossRef PubMed PubMedCentral
  15.  
  16. Tang Z, Li M, Zhang X, Hou W. Dietary flavonoid intake and the risk of stroke: a dose-response meta-analysis of prospective cohort studies. BMJ Open. 2016; 6(6): e008680. CrossRef PubMed PubMedCentral
  17.  
  18. Dovgan IM, Melnyk NO, Labunets IF, Utko NA, Savosko SI. The activity of antioxidant enzymes in rat sciatic nerve following a hemorrhagic stroke. World of Medicine and Biology. 2017; 3(61): 100-07. [Ukrainian]. CrossRef  
  19. Awwad IA, Mobasher AA, Awadallah AMA, Amin RS. The possible neuroprotective effects of amlodipine, magnesium sulphate and vitamin eagainst cerebral ischemia and ischemia/ reperfusion injury in albino rats. Egypt Soc Pharmacol Exp Ther. 2008; 29(2): 429-43.
  20.  
  21. Savosko SI, Chaikovsky JB, Pogorela NKh, Makarenko AN. Features of histostructural changes in rat cerebral cortex in hemorrhagic stroke modeling. Int J Phys Pathophys. 2013; 4(2): 113-21. CrossRef  
  22. Paxinos G, Watson C. The rat brain in stereotaxic coodiates. San Diego: Academic Press, 2008.
  23.  
  24. Vereshchaka IV, Bulgakova NV, Kornieiev VV, Chaikovskii KA, Maznychenko AV. Modulation of the monosynaptic reflex potentialsin the decerebrated rats under theinfluence of hydroxytryptophan. Fiziol Zh. 2016; 62(2): 41-6. [Ukrainian]. CrossRef PubMed
  25.  
  26. Kolomiytsev AK, Chaikovsky YuB, Tereshchenko TL. A rapid method of impregnation of elements of the peripheral nervous system with silver nitrate, suitable for paraffin and celloidin sections. Arch Anat. 1981; 81(8): 93-6. [Russian].
  27.  
  28. Qureshi AI, Ali Z, Suri MF, et al. Extracellular glutamate and other amino acids in experimental intracerebral hemorrhage: an in vivo microdialysis study. Crit Care Med. 2003; 31: 1482-89. CrossRef PubMed
  29.  
  30. Thomalla G, Glauche V, Weiller C, Rother J. Time course of wallerian degeneration after ischaemic stroke revealed by diffusion tensor imaging. JNNP. 2005; 76(2): 266-8. CrossRef  
  31. Wasserman JK, Schlichter LC. White matter injury in young and aged rats after intracerebral hemorrhage. Exp Neurol. 2008; 214: 266-75. CrossRef PubMed
  32.  
  33. Melnyk N, Dovgan I, Savosko S. Sciatic nerve injury at intracerebral hemorrhage in internal capsule in rats. Modern Science – Moderní věda. 2016; 1: 136-41.
  34.  
  35. Liu AM, Lu G, Tsang KS, Li G, Wu Y, Huang ZS, Ng HK, Kung HF, Poon WS. Umbilical cord-derived mesenchymal stem cells with forced expression of hepatocyte growth factor enhance remyelination and functional recovery in a rat intracerebral hemorrhage model. Neurosurgery. 2010; 67: 357-66. CrossRef PubMed
  36.  
  37. Ni W, Okauchi M, Hatakeyama T, Gu Y, Keep RF, Xi G, Hua Y. Deferoxamine reduces intracerebral hemorrhageinduced white matter damage in aged rats. Exp Neurol. 2015; 272: 128-34. CrossRef PubMed PubMedCentral
  38.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.