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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(6): 68-76

THE INFLUENCE OF LOW-LEVEL LIGHT RADIATION OF RED SPECTRUM DIAPASON ON GLYCEMIC PROFILE AND PHYSICOCHEMICAL CHARACTERISTICS OF RAT’S ERYTHROCYTES IN DIABETES MELLITUS

O.I. Karmash1, M.Y. Lіuta1, N.V. Yefimenko1, A.M. Korobov2, N.O. Sybirna1

  1. Ivan Franko National University of Lviv, Ukraine
  2. V.N. Karazin Kharkiv National University, Ukraine
DOI: https://doi.org/10.15407/fz64.06.068


Abstract

The influence of low-level light therapy (LLLT) on glycemic indices and physicochemical characteristics of rat’s periphery blood erythrocytes in streptozotocin-induced (60 mg/kg) diabetes mellitus (DM) was investigated. The animals were irradiated by light with wavelength of 630 nm and power of 150 mW with the device analogous to the phototherapeutic apparatus “Barva” (duration of irradiation was 5 min in the course of 10 days). The glucose concentration in whole blood and content of glycosylated hemoglobin in erythrocytes decreased by 37.02 and 15.7 %, respectively, in irradiated DM rats. The irradiation of healthy animals causes the increasing of hemoglobin concentration and reticulocytes content by 26.68 and 41.8 %, respectively, comparing to control. It was revealed that irradiation causes an increase of erythrocyte number and hemoglobin concentration by 20.13 and 20.27 % in DM animals, respectively, and a decrease of reticulocyte number by 45.53 % comparing to the analogues indices in non-irradiated DM rats. The normalized effect of LLLT on erythrocyte hemolysis indices in DM rats were established. LLLT causes a decrease in the level of TBA-positive products in blood plasma and hemolysates of erythrocytes in 1.8 times in healthy animal group. Irradiation of DM rats induces a decrease of TBA-positive products content in plasma and hemolysates of erythrocytes in 1.2 and 1.3 times, respectively, compared to analogues indices in non-irradiated animals.

Keywords: experimental diabetes mellitus, low-level light therapy, peripheral blood erythrocytes

Full text: (PDF, in Ukrainian)

References

  1. International Diabetes Federation. Diabetes Atlas 7th Edition. 2015.
    2.  
  2. Anders JJ, Lanzafame RJ, Arany PR. Low-level light/laser therapy versus photobiomodulation therapy. Photomed Laser Surg. 2015; 33(4): 183-4. CrossRef PubMed PubMedCentral
    3.  
  3. Korobov AM. About terminology in photomedicine and its results. Photobiology and Photomedicine. 2017; 14(1,2): 11-2. [Ukrainian].
    4.  
  4. Langella LG, Silva PFC, Costa-Santos L, Gonçalves MLL, Motta LJ, Deana AM, Fernandes KPS, MesquitaFerrari RA, Bussadori SK. Photobiomodulation versus light-emitting diode (LED) therapy in the treatment of temporomandibular disorder: study protocol for a randomized, controlled clinical trial. Trials. 2018; 19(1):71. CrossRef PubMed PubMedCentral
    5.  
  5. Cornejo-Garrido J, Becerril-Chávez F, Carlín-Vargas G, Ordo-ez-Rodríguez JM, Abrajan-González Mdel C, de la Cruz-Ramírez R2 Ordaz-Pichardo C. Antihyperglycaemic effect of laser acupuncture treatment at BL20 in diabetic rate. Acupunct Med. 2014; 32: 486-94. CrossRef PubMed
    6.  
  6. Kazemi Khoo N, Iravani A, Arjmand M, Vahabi F, Lajevardi M, Akrami SM, Zamani Z. A metabolomic study on the effect of intravascular laser blood irradiation on type 2 diabetic patient. Lasers Med Sci. 2013; 28: 1527-32. CrossRef PubMed
    7.  
  7. Kushibiki T, Okawa S, Hirasawa T, Ishihara M. Optogenetic control of insulin secretion by pancreatic β-cells in vitro and in vivo. Gene Ther. 2015; 22(7): 553-9. CrossRef PubMed
    8.  
  8. Lim J, Sanders RA, Snyder AC, Eells JT, Henshel DS, Watkins JB. Effects of low-level light therapy on streptozotocin-induced diabetic kidney. J Photochem Photobiol B. 2010; 99(2): 105-10. CrossRef PubMed
    9.  
  9. Lim J, Ali ZM, Sanders RA, Snyder AC, Eells JT, Henshel DS, Watkins JB. Effects of low-level light therapy on hepatic antioxidant defense in acute and chronic diabetic rats. J Biochem Mol Toxicol. 2009; 23(1): 1-8. CrossRef PubMed
    10.  
  10. Lan CC, Wu SB, Wu CS, Shen YC, Chiang TY, Wei YH, Yu HS. Induction of primitive pigment cell differentiation by visible light (helium-neon laser): a photoacceptorspecific response not replicable by UVB irradiation. J Mol Med (Berl). 2012; 90 (3): 321-30. CrossRef PubMed
    11.  
  11. Huang L, Jiang X, Gong L, Xing D. Photoactivation of Akt1/GSK3β Isoform-Specific Signaling Axis Promotes Pancreatic β-Cell Regeneration. J Cell Biochem. 2015; 116(8): 1741-54. CrossRef PubMed
    12.  
  12. Frangez I, Cankar K, Ban Frangez H, Smrke DM. The effect of LED on blood microcirculation during chronic wound healing in diabetic and non-diabetic patients-a prospective, double-blind randomized study. Lasers Med Sci. 2017; 32(4): 887-94. CrossRef PubMed
    13.  
  13. Ivanova YUV, Klimova EM, Prasol VA, Mushenko EV, Korobov AM, Pogorelov MV, Timchenko DS, Serbin ME Phototherapy in the complex of treatment at the plastic closure of wounds in patients with ischemic and neuroischemic form of diabetic foot. Photobiology and photomedicine. 2018; 1: 22-7. [Ukrainian].
    14.  
  14. Rundo AI, Kosinets VA. Application of the combined phototherapy in complex treatment of patients with complications of diabetic foot syndrome. Novosti Khirurgii. 2016; 24 (2): 131-37. [Russian]. CrossRef  
  15. Buko V, Zavodnik I, Kanuka O, Belonovskaya E, Naruta E, Lukivskaya O, Kirko S, Budryn G, Żyżelewicz D, Oracz J, Sybirna N. Antidiabetic effects and erythrocyte stabilization by red cabbage extract in streptozotocin-treated rats. Food Funct. 2018; 9(3):1850-63. CrossRef PubMed
    16.  
  16. Novitskii VV, Ryazantseva NV, Stepovaia EA. Molecular impairments of erythrocyte`s membrane during the pathologies of different genesis is the typical reaction of organism: contours of problem. Bulletin of Siberian medicine. 2006; (2): 62-9. [Russian].
    17.  
  17. Sybirna NO, Burda VA, Chajka YaP. Methods of blood system research. Lviv: LNU, 2006. 100 p. [Ukrainian].
    18.  
  18. Timirbulatov RA, Seleznev EI. Method for increasing the intensity of free radical oxidation of lipid-containing components of the blood and its diagnostic significance. Lab Delo. 1981; (4): 209-11. [Russian]. PubMed
    19.  
  19. Alam U, Asghar O, Azmi S, Malik RA. General aspects of diabetes mellitus. Handb Clin Neurol. 2014; 126: 211-22. CrossRef PubMed
    20.  
  20. Shui S, Wang X, Chiang JY, Zheng L. Far-infrared therapy for cardiovascular, autoimmune, and other chronic health problems: A systematic review. Exp Biol Med (Maywood). 2015; 240(10): 1257-65. CrossRef PubMed PubMedCentral
    21.  
  21. Chernysh PP, Kayumov UK, Khaidarova FA, Maksutova NN. Genesis of insulin resistance in type 2 diabetes: is it herediatary or cortisol dependet? Ukrainian Therapeutic J. 2014; 2: 65-9. [Ukrainian].
    22.  
  22. Goyal SN, Reddy NM, Patil KR, Nakhate KT, Ojha S, Patil CR, Agrawal YO. Challenges and issues with streptozotocin-induced diabetes - A clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics. Chem Biol Interact. 2016; 244: 49-63. CrossRef PubMed
    23.  
  23. Dos Santos SA, Dos Santos Vieira MA, Simões MCB, Serra AJ, Leal-Junior EC, de Carvalho PTC. Photobiomodulation therapy associated with treadmill training in the oxidative stress in a collagen-induced arthritis model. Lasers Med Sci. 2017; 32(5): 1071-79. CrossRef PubMed
    24.  
  24. Vladimirov YA, Gorbatenkova EA, Paramonov NV, Azizova OA. Photoreactivation of superoxide dismutase by intensive red (laser) light. Free Radic Biol Med. 1988; 5(5-6): 281-6. CrossRef

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