THE INFLUENCE OF LOW-LEVEL LIGHT RADIATION OF RED SPECTRUM DIAPASON ON GLYCEMIC PROFILE AND PHYSICOCHEMICAL CHARACTERISTICS OF RAT’S ERYTHROCYTES IN DIABETES MELLITUS
O.I. Karmash1, M.Y. Lіuta1, N.V. Yefimenko1, A.M. Korobov2, N.O. Sybirna1
- Ivan Franko National University of Lviv, Ukraine
- V.N. Karazin Kharkiv National University, Ukraine
DOI: https://doi.org/10.15407/fz64.06.068
Abstract
The influence of low-level light therapy (LLLT) on glycemic
indices and physicochemical characteristics of rat’s periphery
blood erythrocytes in streptozotocin-induced (60 mg/kg)
diabetes mellitus (DM) was investigated. The animals were
irradiated by light with wavelength of 630 nm and power of
150 mW with the device analogous to the phototherapeutic
apparatus “Barva” (duration of irradiation was 5 min in the
course of 10 days). The glucose concentration in whole blood
and content of glycosylated hemoglobin in erythrocytes decreased
by 37.02 and 15.7 %, respectively, in irradiated DM
rats. The irradiation of healthy animals causes the increasing
of hemoglobin concentration and reticulocytes content by
26.68 and 41.8 %, respectively, comparing to control. It was
revealed that irradiation causes an increase of erythrocyte
number and hemoglobin concentration by 20.13 and 20.27 %
in DM animals, respectively, and a decrease of reticulocyte
number by 45.53 % comparing to the analogues indices in
non-irradiated DM rats. The normalized effect of LLLT on
erythrocyte hemolysis indices in DM rats were established.
LLLT causes a decrease in the level of TBA-positive products
in blood plasma and hemolysates of erythrocytes in 1.8 times in
healthy animal group. Irradiation of DM rats induces a decrease
of TBA-positive products content in plasma and hemolysates
of erythrocytes in 1.2 and 1.3 times, respectively, compared
to analogues indices in non-irradiated animals.
Keywords:
experimental diabetes mellitus, low-level light therapy, peripheral blood erythrocytes
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