Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(6): 40-46

Spatial dimension of nigral astrogliosis observed in rotenone model of Parkinson’s disease

A.O. Bogdaniuk, A.G. Nikonenko

    Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine


This paper addresses spatial aspects of reactive astrogliosis observed in the substantia nigra pars compacta (SNc) in rotenone model of Parkinson’s disease. The study was performed on adult male Wistar rats receiving the intracerebral 12 µg rotenone infusion into the left SNc. SNc tissue was analyzed 40, 70 and 200 days after the infusion. It was shown that rotenone caused the increase in astocyte density at all analyzed time-points, thus indicating the proliferation of the cells. Radial distribution function (RDF) analysis demonstrated a non-uniform spatial distribution of nigral astrocytes. Their density was revealed to be higher in the proximity of neuronal cell bodies, especially on the infused side. In addition, RDF analysis provided evidence of spatial clusters of astrocytes 40 days after the infusion. The results of this study suggest that nigral astrogliosis observed in the rotenone model of Parkinson’s disease includes changes in spatial distribution of astrocytes. These changes reflect the rearrangement of SNc tissue architecture occurring in response to rotenone-related degeneration of SNc neurons.

Keywords: animal model; astrocytes; Parkinson’s disease; rotenone; spatial pattern


  1. Khakh BS, Sofroniew MV. Diversity of astrocyte functions and phenotypes in neural circuits. Nat Neurosci. 2015; 18(7):942-52. CrossRef PubMed PubMedCentral
  3. Attwell D, Buchan AM, Charpak S, Lauritzen M, Macvicar BA, Newman EA. Glial and neuronal control of brain blood flow. Nature. 2010; 468 (7321): 232-43. CrossRef PubMed PubMedCentral
  5. Barker AJ, Ullian EM. Astrocytes and synaptic plasticity. Neuroscientist. 2010; 16 (1): 40-50. CrossRef PubMed
  7. Stogsdill JA, Ramirez J, Liu D, Kim YH, Baldwin KT, Enustun E, Ejikeme T, Ji RR, Eroglu C. Astrocytic neuroligins control astrocyte morphogenesis and synaptogenesis. Nature. 2017; 551 (7679): 192-7. CrossRef PubMed PubMedCentral
  9. Haydon PG, Carmignoto G. Astrocyte control of synaptic transmission and neurovascular coupling. Physiol Rev. 2006; 86 (3): 1009-31. CrossRef PubMed
  11. López-Hidalgo M, Hoover WB, Schummers J. Spatial organization of astrocytes in ferret visual cortex. J Comp Neurol. 2016; 524 (17): 3561-76. CrossRef PubMed PubMedCentral
  13. Tsai HH, Li H, Fuentealba LC, Molofsky AV, TaveiraMarques R, Zhuang H, Tenney A, Murnen AT, Fancy SP, Merkle F, Kessaris N, Alvarez-Buylla A, Richardson WD, Rowitch DH. Regional astrocyte allocation regulates CNS synaptogenesis and repair. Science. 2012; 337 (6092): 358-62. CrossRef PubMed PubMedCentral
  15. Voitenko LP, Nikonenko AG. [Modification of experimental rotenone model of Parkinson's disease]. Fiziol Zh. 2015; 61 (1): 83-90. [Ukrainian]. CrossRef PubMed
  17. Barker AJ, Ullian EM. New roles for astrocytes in developing synaptic circuits. Commun Integr Biol. 2008; 1 (2): 207-11. CrossRef  
  18. Burda JE, Bernstein AM, Sofroniew MV. Astrocyte roles in traumatic brain injury. Exp Neurol. 2016; 275 (Pt 3): 305-15. CrossRef PubMed PubMedCentral
  20. Pacelli C, Giguere N, Bourque MJ, Levesque M, Slack RS, Trudeau LE. Elevated mitochondrial bioenergetics and axonal arborization size are key contributors to the vulnerability of dopamine neurons. Curr Biol. 2015; 25 (18): 2349-60. CrossRef PubMed
  22. Ge WP, Miyawaki A, Gage FH, Jan YN, Jan LY. Local generation of glia is a major astrocyte source in postnatal cortex. Nature. 2012; 484 (7394): 376-80. CrossRef PubMed PubMedCentral
  24. Kumar S, Yin X, Trapp BD, Paulaitis ME, Hoh JH. Role of long-range repulsive forces in organizing axonal neurofilament distributions: evidence from mice deficient in myelin-associated glycoprotein. J Neurosci Res. 2002; 68 (6): 681-90. CrossRef PubMed
  26. Gathercole DV, Colling DJ, Skepper JN, Takagishi Y, Levi AJ, Severs NJ. Immunogold-labeled L-type calcium channels are clustered in the surface plasma membrane overlying junctional sarcoplasmic reticulum in guinea-pig myocytesimplications for excitation-contraction coupling in cardiac muscle. J Mol Cell Cardiol. 2000; 32 (11): 1981-94. CrossRef PubMed
  28. Nikonenko I, Nikonenko A, Mendez P, Michurina TV, Enikolopov G, Muller D. Nitric oxide mediates local activity-dependent excitatory synapse development. Proc Natl Acad Sci U S A. 2013; 110 (44): E4142-51. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.