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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(6): 32-39


THE OPTIMIZATION METHOD OF ISOLATION OF IMMATURE DENDRITIC CELLS

A.N. Goltsev, T.G. Dubrava, E.E. Yampolskaya, Yu.A. Gayevskaya, N.N. Babenko, N.A. Bondarovich, I.G. Kovalenko

    Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz64.06.032

Abstract

The method of isolation of bone marrow mononuclear cells in «trasograph» gradient, enabling to obtain more than 40 % of cells, among those 28 % were CD14+-monocytes has been presented. The minimum set of phenotype markers to assess immature dendritic cells in vitro has been selected. In a comparative aspect it has been shown that the most adequate sources of obtaining of dendritic cells are mononuclear cells in contrast to total cell suspension of bone marrow. It has been found that the differentiation of myeloid progenitor cells and monocytes into immature dendritic cells was accompanied with the loss of CD14 antigen , minimization of expression rate of co-stimulatory molecule CD86 and appearance of CD11b-antigen marker, which is the feature of these cells. The results allow not only to estimate the qualitative characteristics of the obtained dendritic cells and their tolerogenic activity in the model of autoimmune diseases, but also to underline the perspectives of establishing their stocks at low temperature banks to be used in clinics.

Keywords: dendritic cells; bone marrow; mononuclear cells, monocytes; co-stimulatory molecules

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