EFFECTS OF VENOMS ON LARGECONDUCTANCE CATIONIC CHANNELS ON THE INNER NUCLEAR MEMBRANE OF RAT CEREBELLUM PURKINJE NEURONS
O.V. Lunko, O.A. Fedorenko, E.V. Isaevа, S.M. Marchenko
Bogomoletz Institute of Physiology National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.06.023
![](/images/cite.png)
Abstract
Large-conductance cationic channels (LCC) expressed on the
Pukinje neuron nuclei of the rat cerebellum, characterized by
selectivity to monovalent cations, slow kinetics and potentialdependent
activity. The identification of a specific LCC
channel blocker is the first step in determining their structure
and physiological role. In order to identify components that
specifically interact with LCC channels on the inner nuclear
membrane, we investigated the effects of venoms of five
animals: forest scorpion (Heteromerus laoticus), monocled
cobra (Naja kaouthia), steppe viper (Vipera renardi), banded
krait (Bungarus fasciatus), and puff adder (Bitis arietans) on
the characteristics of these channels. It has been shown that
all investigated venoms significantly reduce the open state
probability of LCC channels in average by 76 - 86%. The
application venoms of H. laoticus and B. fasciatus leads to a
decrease in the currents amplitude of LCC channels, by 43%
and 26% respectively. The influence of other compounds as
blocker of LCC channels was less pronounced. Obtained data
can be used for further direct search of specific and high affinity
blockers of these channels.
Keywords:
nuclear envelope; ion channels blocker; venoms
References
- Marchenko SM, Yarotskyy V V., Kovalenko TN, Kostyuk PG, Thomas RC. Spontaneously active and InsP3-activated ion channels in cell nuclei from rat cerebellar Purkinje and granule neurones. J Physiol. 2005; 565:897-910.
CrossRef
PubMed PubMedCentral
- Fedorenko O, Yarotskyy V, Duzhyy D, Marchenko SM. The large-conductance ion channels in the nuclear envelope of central neurons. Pflug Arch. 2010; 460:1045-50.
CrossRef
PubMed
- Lun'ko OV, Fedorenko OA, Marchenko SM. The effect of Ca2+ on the properties of the large conductance cation channels of the nuclear envelope of the cerebellar neurons. Fiziol Zh. 2013; 59:28-32.
CrossRef
PubMed
- Fedorenko OA, Marchenko SM. Ion channels of the nuclear membrane of hippocampal neurons. Hippocampus. 2014; 24:869-76.
CrossRef
PubMed
- Fedorenko EA, Semenova OV, Marchenko SM. Properties of Large-Conductance Cationic Channels in the Neuronal Nuclear Envelope. Neurophysiol. 2011; 43:192-4.
CrossRef
- Fedorenko EA, Dyzhii DE, Marchenko SM. Largeconductance cationic channels in the nuclear envelope of Purkinje neurons from the rat cerebellum. Neurophysiol. 2007; 39:99-104.
CrossRef
- Dutertre S, Lewis RJ. Use of venom peptides to probe ion channel structure and function. J Biol Chem. 2010; 285:13315-20.
CrossRef
PubMed PubMedCentral
- Cruz LJ, Luo S. Toxins and Drug Discovery. 2017.
- Nasiripourdori A, Taly V, Grutter T, Taly A. From toxins targeting ligand gated ion channels to therapeutic molecules. Toxins. 2011; 3:260-93.
CrossRef
PubMed PubMedCentral
- Gorbacheva EV, Starkov VI, Tsetlin VI, Utkin YN, Vulfus CA. Viperidae snake venoms block nicotinic acetylcholine receptors and voltage-gated Ca 2+ channels in identified neurons of fresh-water snail Lymnaea stagnali. Biochem Suppl Ser A Membr Cell Biol. 2008; 2:14-8.
- Harvey AL, Anderson AJ. Dendrotoxins: Snake toxins that block potassium channels and facilitate neurotransmitter release. Pharmacol Ther. 1985; 31:33-55.
CrossRef
- Vulfius CA, Spirova EN, Serebryakova MV. et al. Peptides from puff adder Bitis arietans venom, novel inhibitors of nicotinic acetylcholine receptors. Toxicon. 2016; 121:70-6.
CrossRef
PubMed
- Lunko O V., Grushkovska I V., Lun'ko OO, Marchenko SM. Effect of Tubocurarine on Large-Conductance Cationic Channels in the Inner Nuclear Membrane of Purkinje Neurons of the Rat Cerebellum. Neurophysiol. 2016; 48:332-5.
CrossRef
- Vandendriessche T, Kopljar I, Jenkins DP, et al. Purification, molecular cloning and functional characterization of HelaTx1 (Heterometrus laoticus): The first member of a new κ-KTX subfamily. Biochem Pharmacol. 2012; 83:1307-17.
CrossRef
PubMed PubMedCentral
- Quintero-Hernández V, Jiménez-Vargas JM, Gurrola GB, Valdivia HH, Possani LD. Scorpion venom components that affect ion-channels function. Toxicon. 2013; 76:328-42.
CrossRef
PubMed PubMedCentral
- Hille B. Ionic Channels of Excitable Membranes (3rd ed). 2001.
- Schroeder I. How to resolve microsecond current fluctuations in single ion channels: The power of beta distributions. Channels. 2015; 9:262-80.
CrossRef
PubMed PubMedCentral
- Fitzhugh R. Statistical properties of the asymmetric random telegraph signal, with applications to singlechannel analysis. Math Biosci. 1983; 64:75-89.
CrossRef
- Yellen G. Ionic permeation and blockade in Ca2+-activated K+ channels of bovine chromaffin cells. J Gen Physiol. 1984; 84:157-86.
CrossRef
PubMed
|