Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(4): 8-15


Effects of stimulation and blockade of the synthesis of endogenous hydrogen sulfide at myocardial ischemia-reperfusion

Sahach VF, Shymans'ka TV, Hoshovs'ka IuV

    O.O.Bogomoletz Institute of Physiology National Academy of Science of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz59.04.008

Abstract

In experiments on isolated rat hearts, perfused according to Langendorff method, the effects of stimulation of the synthesis and blockade of endogenous hydrogen sulfide in myocardial ischemia-reperfusion (20 min/40 min) was studied. L-cysteine (121 mg/kg), precursor of endogenous hydrogen sulfide was injected intraperitoneally 30 minutes before the experiment without and within 10 minutes after administration of DL-propargylglycine (11.3 mg/kg) ("Sigma", USA)--inhibitor of cystathionine-gamma-lyase. The heart function was assessed by measuring the LVDP, dP/dt, coronary flow, heart rate. The opening of mitochondria permeability transition (MPT) pore was estimated by releasing of a stable factor with UV absorbance (lambda(max) 250 nm) into the coronary outflow probes during the initial phase of reperfusion. Administration L-cysteine was accompanied by a decrease of reperfusion disorders in cardiac function compared to control rats. The results showed that L-cysteine pretreated hearts against the blockade of cystathionine-gamma-lyase with DL-propargylglycine exerted a powerful cardioprotective effect in ischemia-reperfusion injury. Significant post-ischemic recover of heart function and improving the efficiency of oxygen metabolism was accompanied with tiny quantity of mitochondrial factor releasing comparing to I/R group. Positive influence of the combined DL-propargylglycine and L-cysteine action was the prevention of MPT pore opening.

Keywords: hydrogen sulfide, L-cysteine, DL-propargylglycine, isolated heart, ischemia-reperfusion, mitochondrial permeability transition pore

References

  1. Sagach V.F., Shimans'ka T.V., Nadtochii S.M. Poperedzhennya postreperfuziinih porushen' funktsii sertsya ta neefektivnogo vikoristannya kisnyu za dopomogoyu ingibitoriv mitohondrial'noi pori . Fiziol. zhurn. 2002. 48, N 6. P. 3-9.
  2.  
  3. Sagach V.F., Shimans'ka T.V., Nadtochii S.M. Faktor, yakii vivil'nyuiet'sya pid chas reperfuzii ishemizovanogo sertsya, mozhe buti markerom vidkrittya mitohondrial'noi pori . Tam samo. 2003. 49, N 4. P. 6-12.
  4.  
  5. Sagach V.F, Vavilova G.L., Rudik O.V., Dobrovol's'kii F.V., Shimans'ka T.V., Medviediev O.S. Ingibuvannya mitohondrial'noi pori yak odin iz mehanizmiv kardioprotektornoi dii koenzimu Q10 . Tam samo. 2007. 53, N 4. P. 34-41.
  6.  
  7. Strutins'ka N.A., Semenihina O.M., Chorna S.V., Vavilova G.L., Sagach V.F. Sirkovoden' prignichuie kal'tsiiindukovane vidkrivannya mitohondrial'noi pori u sertsi doroslih i starih shchuriv . Tam samo. 2011. 57, N 6. P. 3-13.
  8.  
  9. Shimanskaya T.V, F.V.Dobrovol'skii, G.L.Vavilova, N.A.Strutinskaya, E.V.Rudik, Sagach V.F. NO-zavisimaya modulyatsiya chuvstvitel'nosti otkritiya mitohondrial'noi pori pri ishemii-reperfuzii izolirovannogo serdtsa . Ros. fiziol. zhurn. im. I.M. Sechenova. 2009. 95, N 1. P.28-37.
  10.  
  11. Shimans'ka T.V., Goshovs'ka Yu.V., Semenihina O.M., Sagach V.F. Vpliv sirkovodnyu na reaktsii izol'ovanogo sertsya shchuriv pri navantazhenni ob'iemom i ishemii-reperfuzii . Fiziol. zhurn. 2012. 58, N 6. p.57-66.
  12.  
  13. Shimanskaya T.V, Strutinskaya N.A., Vavilova G.L., Goshovskaya Yu.V., Semenihina E.N., Sagach V.F. Tsiklosporin A-chuvstvitel'naya mitohondrial'naya pora kak mishen' kardioprotektornogo deistviya donora serovodoroda . Ros. fiziol. zhurn. im. I.M. Sechenova. 2013. 99, N 2. P.261-272.
  14.  
  15. Camara A.K.S., Bienengraeber M., Stowe D.F. Mitochondrial approaches to protect against cardiac ischemia and reperfusion injury . Front Physiol. 2011. p.2-13. CrossRef  
  16. Dongo E., Hornyak I., Benko Z., Kiss L. The cardioprotective potential of hydrogen sulfide in myocardial ischemia/reperfusion injury . Acta Physiol. Hung. 2011. 98, N 4. P.369-381. CrossRef PubMed
  17.  
  18. Elrod J.W., Calvert J.W., Morrison J., Doeller J.E., Kraus D.W., Tao L., Jiao X., Scalia R., Kiss L., Szabo C., Kimura H., Chow C.W., Lefer D.J. Hydrogen sulfide attenuates myocardial ischemia-reperfusion injury by preservation of mitochondrial function . Proc. Natl. Acad. Sci. USA. 2007. 104. P.15560-15565. CrossRef PubMed PubMedCentral
  19.  
  20. Elsey D.J.,Fowkes R.C., Baxter G.F. L-cysteine stimulates hydrogen sulfide synthesis in myocardium associated with attenuation of ischemia-reperfusion injury . J. Cardiovasc. Pharmacol. Ther. 2010. 15, N 1. P. 53-59. CrossRef PubMed
  21.  
  22. Jha S., Calvert J.W., Duranski M.R., Ramachandran A., Lefer D.J. Hydrogen sulfide attenuates hepatic ischemiareperfusion injury: role of antioxidant and antiapoptotic signaling . Amer. J. Physiol. Heart. Circ. Physiol. 2008 295, N 2. p.N801-N806. CrossRef PubMed PubMedCentral
  23.  
  24. Kimura H. Hydrogen sulfide: Its production and functions . Exp. Physiol. 2011. 96, N 9. P. 833-835. CrossRef PubMed
  25.  
  26. Kimura, Y., Goto, Y., Kimura, H. Hydrogen sulfide increases glutathione production and suppresses oxidative stress in mitochondria. Antioxid. Redox Signal. 2010. 12. P.1-13. CrossRef PubMed
  27.  
  28. Lefler C.W., Parfenova H., Basuroy S., Umstor E.S., Fedinec A.L. Hydrogen sulfide and cerebralmicrovascular tone in newborn pigs. Amer. J. Physiol. Heart Circ. Physiol. 2011. 300. P.H440-H447. CrossRef PubMed PubMedCentral
  29.  
  30. Monjok E.M., Kulkarni K.H., Kouamou G., McKoy M., Opere C.A., Bongmba O.N., Njie Y.F., Ohia S.E. Inhibitory action of hydrogen sulfide on muscarinic receptor-induced contraction of isolated porcine irides . Exp. Eye Res. 2008. 87, N 6. p. 612-616. CrossRef PubMed
  31.  
  32. Moody B.F., Calvert J.W. Emergent role of gasotransmitters in ischemia-reperfusion injury . Med.Gas Res. 2011. 1, N 1. P. 3. CrossRef PubMed PubMedCentral
  33.  
  34. Sagach VF, Scrosati M, Fielding J, Rossoni G, Galli C, Visioli F. The water-soluble vitamin E analogue Trolox protects against ischaemia/reperfusion damage in vitro and ex vivo. A comparison with vitamin E . Pharmacol Res. 2002. 45, N 6. P.435-439. CrossRef PubMed
  35.  
  36. Simon F., Giudici R, Duy CN, Schelzig H, Oter S, Gröger M, Wachter U, Vogt J, Speit G, Szabó C, Radermacher P, Calzia E. Hemodynamic and metabolic effects of hydrogen sulfide during porcine ischemia/reperfusion injury . Shock. 2008. 30, N 4. P.359-364. CrossRef PubMed
  37.  
  38. Xue M., Cui J., Xia W., Li Y., Qian L.B., Ye Z.G., Wang H.P., Xia Q. Effect of S-allyl-L-cysteine on isolate heart subject to ischemia/reperfusion . Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2011. 27, N 1. p. 13-17. PubMed
  39.  
  40. Wang R. Physiological implications of hydrogen sulfide: a whiff exploration that blossomed . Physiol.Rev. 2012. 92. P.791-896. CrossRef PubMed
  41.  
  42. Yong Q.C., Hu L-F., Wang S., Huang D., Bian J.S. Hydrogen sulfide interacts with nitric oxide in the heart: possible involvement of nitroxyl . Cardiovasc Res. 2010. 88, N 3. P.482-491. CrossRef PubMed
  43.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.