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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(5): 70-76


INVESTIGATION OF THE EFFECT OF MAGNESIUM IONS ON MORPHOLOGICAL CHANGES OF RAT PANCREAS

R.V. Yanko, O.G. Chaka, M.I. Levashov

    O.O. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.05.070

Abstract

Morphological changes in the pancreas and magnesium concentration in the bones and blood of adult male Wistar rats after daily administration of magnesium ions were studied. The rats were divided into two groups (12 animals each): I – control, II – rats, which daily (for 21 days) received magnesium chloride in a dose of 50 mg / kg. At the end of the experiment we observed a significant increase in the magnesium concentration in the tibia, serum and erythrocytes in the experimental rats by 36, 16 and 17 %, respectively. It was found that administration of magnesium chloride led to morphological changes in the exocrine part of the pancreas, indicating an increase in its activity. Thus, in the gland of experimental rats, we detected exocrine cells of an increased size. The sizes of their nucleus and cytoplasm as well as the number of nucleolus in the nucleus were also increased. The influence of magnesium chloride significantly increased the activity of the endocrine pancreas. This was indicated by the following morphological changes: an increase in the relative area of the endocrine part (by 19 %), the ​​ size of the Langerhans islets, an increase in the number (by 54 %) and density (by 20 %) of the endocrinocytes housed in them compared to the control. Thus, the obtained morphological results indicate that the introduction of magnesium enhances the exo- and endocrine function of the pancreas.

Keywords: magnesium; pancreas

References

  1. Fedyukovich N. Anatomy and physiology human: Textbook. Rostov: Phoenix. 2002. [Russian].
  2.  
  3. Jahnen-Dechent W, Ketteler M. Magnesium basics. Clin Kidney J. 2012; 5(1): i3–i14. CrossRef PubMed PubMedCentral
  4.  
  5. Andrea M. Magnesium in health and disease. Metal ions in life sciences. 2013; 13: 49-79. CrossRef PubMed
  6.  
  7. Fox C, Ramsoomair D, Carter C. Magnesium: its proven and potential clinical significance. South Med J. 2003; 94(12):1195-201. CrossRef  
  8. Mooren F, Turi S, Gunzel D, Schlue W, Domschke W, Singh J, Lerch M. Calcium–magnesium interactions in pancreatic acinar cells. The FASEB J. 2001; 15:659-72. CrossRef PubMed
  9.  
  10. Hersh T, Siddiqui D. Magnesium and the pancreas. Am J Clin Nutr.1973; 26(3): 362-6. CrossRef PubMed
  11.  
  12. Gubergrits N, Yaroshenko L. Dynamics of externalecreter function of pancreas in patients with chronic pancreatitis in combination with chronic bronchitis under the influence of therapy with magnesium. Gastroenterology. 2013; 49  (3): 102. [Russian].
  13.  
  14. Barbagallo M, Dominguez L. Magnesium and type 2 diabetes. World J Diabetes. 2015; 6(10): 1152-57. CrossRef PubMed PubMedCentral
  15.  
  16. Dibaba D, Xun R, Yokota K, White E, He K. Magnesium intake and incidence of pancreatic cancer: the vitamins and lifestyle study. Brit J Cancer. 2015; 113:1615-21. CrossRef PubMed PubMedCentral
  17.  
  18. Papazachariou I, Martinez-Isla A, Efthimiou E, Williamson R, Girgis S. Magnesium deficiency in patients with chronic pancreatitis identified by an intravenous loading test. Clin Chim Acta. 2000; 302(1-2):145-54. CrossRef  
  19. Korzhevsky D. Principles of histological techniques. SPb: SpetsLit. 2010. [Russian].
  20.  
  21. Yanko R, Levashov M. Morphofunctional state of the normo- and hypertensive rats pancreas after influence of intermittent normobaric hypoxia. Rus J Physiol. 2018; 104 (5): 581-9. [Russian].
  22.  
  23. Adeyemi D, Komolafe1 O, Adewole1 O, Obuotor E, Abiodun1 A, Adenowo T. Histomorphological and morphometric studies of the pancreatic islet cells of diabetic rats treated with extracts of Annona muricata. Folia Morphol. 2010; 69(2): 92-100.
  24.  
  25. Yanko R. Morphological changes pancreas after of melatonin's effect in different seasons. Fiziol Zh. 2016; 62(6): 88-94. CrossRef PubMed
  26.  
  27. Verigo L. Variability of connective tissue of the pancreas. (Morpho-experimental research). Author. dissertations.03.00.13. Physiology: Moscow. 2005. [Russian].
  28.  
  29. Gromova O, Kalacheva A, Torshin I, Grustlivaya U, Prozorova N, Egorova E, Grishina T, Sukhanova T, Belinskaya A. About diagnostics of magnesium deficiency. Part 1. Archive of Int Med. 2014; 16 (2): 5-10. [Russian].
  30.  
  31. Kunkel H, Pearson P, Schweiqert B. The photoelectric determination of magnesium in body fluids. Lab Clin Med. 1947; 32(8): 1027-33.
  32.  
  33. Levina O. Homoeostasis of magnesium in patients with ischemic heart disease: the possibilities of medical correction. Author. dissertations. 14.00.06. Cardiology: St. Petersburg, 2004. [Russian].
  34.  
  35. Nikolaeva O, Kovaltsova M, Gorgol N. Morphofunctional characteristics of pancreas of rats with chronic stress. Theor and Exp Med. 2013; 59 (2): 23-7. [Russian].
  36.  
  37. Boisvert F, Konningsbruggen S, Navascues J, Lamond A. The multifunctional nucleolus. Nature Reviews Molecular Cell Biology. 2007; 8(7): 574-85. CrossRef PubMed
  38.  
  39. Moeini-Nodeh S, Rahimifard M, Baeeri M, Abdollahi M. Functional Improvement in Rats' Pancreatic islets using magnesium oxide nanoparticles through antiapoptotic and antioxidant pathways. Biol Trace Elem Res. 2017; 175(1):146-55. CrossRef PubMed
  40.  
  41. Curry D, Joy R, Holley D, Bennett L. Magnesium modulation of glucose-induced insulin secretion by the perfused rat pancreas. Endocrinology. 1977; 101(1):203-8. CrossRef PubMed
  42.  

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