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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(5): 63-69


INFLUENCE OF AMMONIUM PYRROLIDINE DITHIOCARBAMATE ON THE PRODUCTION OF REACTIVE OXYGEN AND NITROGEN SPECIES IN TISSUES OF PERIODONTIUM AND SALIVARY GLANDS IN RATS EXPOSED TO SALMONELLA TYPHI LIPOPOLISACCHARIDE

A.M. Yelins’ka, O.O. Shvaikovs’ka, V.O. Kostenko

    Ukrainian Medical & Stomatological Academy, Poltava, Ukraine
DOI: https://doi.org/10.15407/fz64.05.063

Abstract

We investigated the influence of ammonium pyrrolidine dithiocarbamate (PDTC), an inhibitor of the nuclear translocation of the transcription factor κB (NF-κB), on the sources of production of active forms of reactive oxygen and nitrogen species in the tissues of periodontium and submandibular salivary glands (SG) of rats under the experimental systemic inflammatory response induced by the administration of Salmonella typhi lipopolysaccharide in the dose 0.4 μg/kg 3 times during the 1st week and once a week for the next 7 weeks). Administration of lipopolysaccharide increased the production rate of superoxide anion radical (. О ) by electron transport chains of microsomes (by 38.3 and 41.7 %, respectively) and mitochondria (by 40.5 and 37.6 %) and leukocyte NADPH oxidase (by 32.9 and 70.8 %) in the tissues of periodontium and SG, as well as increased the total activity of NO synthase (in 2.5 and 1.9 times) and the concentration of peroxynitrite ions (by 30.1 and 158.0 %). We found an impairment of the mechanism of autoregulation of the physiological nitric oxide content in the tissues: the activation of nitrate and nitrite reductases occurred in the background of increased activity of NO synthase. The use of PDTC in the dose 76 mg/kg 3 times a week starting from the 30th day of the experiment with applying lipopolysaccharide limited the manifestations of oxidative-nitrosative stress in the periodontium and SG tissues: it decreased . О generation by microsomes (by 18.1 and 20.8 %), by mitochondria (by 19.3 and 22.4 %) and leukocyte NADPH oxidase (by 19.0 and 39.0 %) as well as decreased the total activity of NO synthase (by 38.5 and 33.0 %) and the content of peroxynitrite ions (by 13.9 and 54.7 %).

Keywords: nuclear factor κB; ammonium pyrrolidine dithiocarbamate; lipopolysaccharide-induced systemic inflammatory response; reactive oxygen and nitrogen species; periodontium; salivary glands

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