INFLUENCE OF AMMONIUM PYRROLIDINE DITHIOCARBAMATE ON THE PRODUCTION OF REACTIVE OXYGEN AND NITROGEN SPECIES IN TISSUES OF PERIODONTIUM AND SALIVARY GLANDS IN RATS EXPOSED TO SALMONELLA TYPHI LIPOPOLISACCHARIDE
A.M. Yelins’ka, O.O. Shvaikovs’ka, V.O. Kostenko
Ukrainian Medical & Stomatological Academy, Poltava, Ukraine
DOI: https://doi.org/10.15407/fz64.05.063
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Abstract
We investigated the influence of ammonium pyrrolidine
dithiocarbamate (PDTC), an inhibitor of the nuclear translocation
of the transcription factor κB (NF-κB), on the sources
of production of active forms of reactive oxygen and nitrogen
species in the tissues of periodontium and submandibular
salivary glands (SG) of rats under the experimental systemic
inflammatory response induced by the administration of Salmonella
typhi lipopolysaccharide in the dose 0.4 μg/kg 3 times
during the 1st week and once a week for the next 7 weeks).
Administration of lipopolysaccharide increased the production
rate of superoxide anion radical (.
О ) by electron transport
chains of microsomes (by 38.3 and 41.7 %, respectively) and
mitochondria (by 40.5 and 37.6 %) and leukocyte NADPH
oxidase (by 32.9 and 70.8 %) in the tissues of periodontium
and SG, as well as increased the total activity of NO synthase
(in 2.5 and 1.9 times) and the concentration of peroxynitrite
ions (by 30.1 and 158.0 %). We found an impairment of the
mechanism of autoregulation of the physiological nitric oxide
content in the tissues: the activation of nitrate and nitrite
reductases occurred in the background of increased activity
of NO synthase. The use of PDTC in the dose 76 mg/kg 3
times a week starting from the 30th day of the experiment
with applying lipopolysaccharide limited the manifestations
of oxidative-nitrosative stress in the periodontium and SG tissues:
it decreased .
О generation by microsomes (by 18.1 and
20.8 %), by mitochondria (by 19.3 and 22.4 %) and leukocyte
NADPH oxidase (by 19.0 and 39.0 %) as well as decreased
the total activity of NO synthase (by 38.5 and 33.0 %) and the
content of peroxynitrite ions (by 13.9 and 54.7 %).
Keywords:
nuclear factor κB; ammonium pyrrolidine dithiocarbamate; lipopolysaccharide-induced systemic inflammatory response; reactive oxygen and nitrogen species; periodontium; salivary glands
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