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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(5): 54-62


Influence of melatonin on glutathione system in rats skeletal muscle under alloxan induced diabetes

O.Yu. Kushnir, I.M. Yaremii, V.I. Shvets, N.V. Shvets

    Higher education institution in Ukraine «Bukovinian State Medical University», Chernivtsi, Ukraine
DOI: https://doi.org/10.15407/fz64.05.054

Abstract

The aim was to determine the influence of melatonin on basal levels of glucose (BG), the levels of protein carbonyl content, HbA1c and thiobarbituric acid reactive compounds (TBCRC), reduced glutathione (GSH), activities of glutathione reductase [EC 1.6.4.2] (GR), glutathione peroxidase [EC 1.11.1.9] (GPx), glucose- 6-phosphate dehydrogenase [EC 1.1.1.49] (G-6-PhD) in the muscles of alloxan diabetic rats (DM1T). BG levels in blood of rats with DM1T increased on 139%, while in group of alloxan diabetic rats with impaired glucose tolerance (IGT) – were not differ from control. HbA1c levels in the blood of animals with DM1T and IGT exceeded control by 219% and 123%, the level of protein carbonyl groups – by 76% and 36%, the level of TBCRC – by 58% and 36% respectively. Activities of GR, GPx, G-6-PhD and the level of GSH were decreased on 25%, 18%, 50% and 42% in rats with DM1T while in rats with IGT these indexes (besides GSH) were increased on 37%, 22%, 35% respectively than control. Melatonin lowered the BG level on 56% in DM1T rats in comparison to initial levels. It normalized activities of GR, GPx, G-6-PhD and lipid peroxidation, and protein carbonylation as well as hemoglobin glycosylation, so these indexes did not differ from control. Thus, melatonin has strong potential to regulate glucose homeostasis through enhanced glucose consumption, decreased oxidative stress by activation of the glutathione protection system.

Keywords: melatonin; alloxan diabetes; glutathione system; skeletal muscle; rats

References

  1. Rahimi-Madiseh M, Malekpour-Tehrani A, Bahmani M, Rafieian-Kopaei M. The research and development on the antioxidants in prevention of diabetic complications. Asian Pac J Trop Med. 2016; 9(9):825-31. CrossRef PubMed
  2.  
  3. Lenzen S. The mechanisms of alloxan- and streptozotocininduced diabetes. Diabetologia. 2008; 51(2):216-26. CrossRef PubMed
  4.  
  5. Tang L, Li N, Jian W, Kang Y, Yin B, Sun S, Guo J, Sun L, Ta D. Low-intensity pulsed ultrasound prevents muscle atrophy induced by type 1 diabetes in rats. Skelet Muscle. 2017;7(1):29. CrossRef PubMed PubMedCentral
  6.  
  7. Carrillo-Vico A, Guerrero JM, Lardone PJ, Reiter RJ. A review of the multiple actions of melatonin on the immune system. Endocrine. 2005;27(2):189-200. CrossRef  
  8. Mayo JC, Sainz RM, Tan DX, Hardeland R, Leon J, Rodriguez C, et al. Anti-inflammatory actions of melatonin and its metabolites, N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK) and N1-acetyl-5-methoxykynuramine (AMK), in macrophages. J Neuroimmunol. 2005;165(1- 2):139-49.
  9.  
  10. Rodriguez C, Mayo JC, Sainz RM, Antolin I, Herrera F, Martin V, et al. Regulation of antioxidant enzymes: a significant role for melatonin. J Pineal Res. 2004;36(1):1-9. CrossRef PubMed
  11.  
  12. Kushnir OYu, Yaremii IM, Shvetsv VI, Shvets NV. Influence of melatonin on carbohydrate metabolism in the kidney of alloxan diabetic rats. Fiziol Zh. 2017;63(4):64-71. CrossRef  
  13. Banaei S, Ahmadiasl N, Alihemmati A. Comparison of the Protective Effects of Erythropoietin and Melatonin on Renal Ischemia-Reperfusion Injury. Trauma Mon. 2016; 21(3):e23005. CrossRef PubMed PubMedCentral
  14.  
  15. Pantsiuk K., Kushnir O. Changes of pyruvate kinase activity in the muscle tissue of rats under conditions of alloxan diabetes and administration of melatonin. 28th European Students' Conference: «Genetic Engineering When Chance Meets Choice» (27th – 30th September 2017, Carite –Universitatsmedizin Berlin, Germany). P. 6.
  16.  
  17. Djindjic B, Kostic T, Radovanovic Z, Djindjic N, Lazovic M, Zivic M, Perisic Z, Krstic N. The contributions of fasting and postprandial blood glucose increments to oxidative stress and inflammation in dyslipidemic type 2 diabetic patients with stable ischemic heart disease. Int J Cardiol. 2017;227:611-616. CrossRef PubMed
  18.  
  19. Ceban E, Banov P, Galescu A, Botnari V. Oxidative stress and antioxidant status in patients with complicated urolithiasis. J Med Life. 2016; 9(3): 259-62. PubMed PubMedCentral
  20.  
  21. Vlasova SN, Shabunina EI, Pereslegina IA. The activity of glutathione-dependent enzymes of erythrocytes in chronic liver diseases in children. Laboratory Matter. 1990; 8:19-21.
  22.  
  23. Singh J, Kakkar P. Antihyperglycemic and antioxidant effect of Berberis aristata root extract and its role in regulating carbohydrate metabolism in diabetic rats. J Ethnopharmacol. 2009; 123(1):22-6. CrossRef PubMed
  24.  
  25. Kushnir A, Davydenko I. Influence of melatonin on condition of the Langergans isles of the pancreas in alloxan diabetic rats. World of Medicine and Biology. 2009; 5(4):31-5.
  26.  
  27. Tang L, Li N, Jian W, Kang Y, Yin B, Sun S, Guo J, Sun L, Ta D. Low-intensity pulsed ultrasound prevents muscle atrophy induced by type 1 diabetes in rats. Skelet Muscle. 2017; 7(1):29. CrossRef PubMed PubMedCentral
  28.  
  29. Ullah Asmat, Khan Abad, Khan Ismailb. Diabetes mellitus and oxidative stress-A concise review. Saudi Pharm J. 2016; 24(5): 547-53. CrossRef PubMed PubMedCentral
  30.  
  31. Lin GJ, Huang SH, Chen YW, Hueng DY, Chien MW, Chia WT, Chang DM, Sytwu HK. Melatonin prolongs islet graft survival in diabetic NOD mice. J Pineal Res. 2009; 47(3):284-92. CrossRef PubMed
  32.  
  33. Peschke E, Stumpf I, Bazwinsky I, Litvak L, Dralle H, Mühlbauer E. Melatonin and type 2 diabetes - a possible link? J Pineal Res. 2007;42(4):350-8. CrossRef PubMed
  34.  
  35. Coleman SK, Rebalka IA, D'Souza DM, Hawke TJ. Skeletal muscle as a therapeutic target for delaying type 1 diabetic complications. World J Diabetes. 2015; 6(17):1323-36. CrossRef PubMed PubMedCentral
  36.  

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