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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(5): 26-31


Correlations between polymorphisms of double-strand break DNA repair genes and risk of bronchopulmonary pathology development in hazardous industries workers

T.A. Andrushchenko1, S.V. Goncharov2, V.E. Dosenko2

  1. State Institution «Kundiiev Institute of Occupational Health of the National Academy of Medical Sciences of Ukraine», Kyiv, Ukraine
  2. Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.05.026

Abstract

The study in the group of workers of asbestos-cement plants and miners of allelic variants of XRCC7 (rs7003908) and ATM (rs664677) polymorphisms encoding the double-strand break repair DNA (n=215). These polymorphisms are recognized markers of cancer of various types and localizations, as well as markers of radio sensitivity/resistance in the influence of ionizing radiation. The respondents of the research group were workers of asbestos-cement plants and coal miners in the anamnesis in with bronchopulmonary pathology, the control group consisted of workers without diseases of the respiratory system. The genotypes of the genes of the the XRCC7 (rs7003908) and ATM (rs664677) were determined in real time by the polymerase chain reaction method. As a result of the study, it was established that the minor genotype ATM•T/T (OR=2.48 95%CI: 1.16-5.31; χ²=6.61; P<0.01) are associat with a risk of bronchopulmonary pathology. Also, it was found that the dominant allele of ATM•A (OR=0.69; 95% CI: 0.46-1.04), genotypes: dominant homozygotes АТМ•А/А (ОR=0.83; 95%CI: 0.45-1.54), heterozygotes АТМ•А/Т (ОR=0.67; 95%CI: 0.38-1.21) cause resistance to bronchopulmonary pathology in workers with harmful and dangerous working conditions. The analysis of the frequency distribution of the allelic variants of the gene XRCC7 (rs7003908) suggests that there is no association of this polymorphism with the risk of developing respiratory diseases. The obtained results to the importance of polymorphism of the ATM gene (rs664677) for the repair of double-strand DNA ruptures in the development of diseases of the respiratory system in workers in harmful and dangerous industries.

Keywords: SNP; XRCC7; ATM; bronchopulmonary pathology

References

  1. Pavanello S, Bchir F, Pulliero A. Interaction between CYP1 A2-T2467DELT polymorphism and smoking in adenocarcinoma and squamous cell carcinoma of the lung. Lung Cancer. 2007; 57 (3); 266-72. CrossRef PubMed
  2.  
  3. Andrushchenko TA, Goncharov SV, Dosenko VE Genetic predisposition to bronchopulmonary pathology in workers of harmful and hazardous industries: analysis of five polymorphisms of DNA gene repair. Fiziol Zh. 2018; 64 (4): 12-9. [Ukrainian]. CrossRef  
  4. Litvinov SV, The main repair pathways of double-strand breaks in the genomic DNA and interaction between them. Cytol and Genetics. 2014; 48 (3); 64-77. [Ukrainian]. CrossRef  
  5. Kuschel B, Auranen A, McBride S. et al. Variants in double-strand break repair genes and breast cancer susceptibility. Hum Mol Genet. 2002; (11); 1399-440. CrossRef PubMed
  6.  
  7. Thacker J, Zdzienicka MZ. The XRCC genes: expanding roles in DNA double-strand break repair. DNA Repair (Amst.). 2004; (3); 1081-90. CrossRef PubMed
  8.  
  9. Zienolddiny S, Campa D, Lind H. et al. Polymorphisms of DNA repair genes and risk of non-small cell lung cancer. Carcinogenesis. 2006; 27 (3): 560-7. CrossRef PubMed
  10.  
  11. Rahimi M, Fayaz S, Fard-Esfahani A. et al. The role of Ille 3434Thr XRCC7 gene polymorphism in Differentiated Thyroid Cancer risk in a Iranian population. Iran Biomed J. 2012; 16(4); 218-22. PubMed PubMedCentral
  12.  
  13. Xiao M, Shen Y, Chen L. et al. The rs7003908 (T>G) polymprphism in the XRCC7 gene and the risk of cancers. Mol Biol Rep. 2014; 41(6); 3577-82. CrossRef PubMed
  14.  
  15. Hsieh YH, Chang WS, Tsai CW. et al. DNA double-strand break repair gene XRCC7 genotypes were associated with hepatocellular carcinoma risk in Taiwanese males and alcohol drinkers. Tumour Biol. 2015; 36(6); 4101-6. CrossRef PubMed
  16.  
  17. Nasiri M, Saadat I, Omidvari S. et al. Genetic variation in DNA repair gene XRCC7 (G6721T) and susceptibility to breast cancer. Gene. 2012; 505 (1); 195-7. CrossRef PubMed
  18.  
  19. Wang C, Huang X-Y, Yao J-G. et al. XRCC7 rs7003908 polymorphism and Helicobacter pylori Infection – Related Gastric Antrum Adenocarcinoma. Int J Genomics. 2013; (6); 1246-52.
  20.  
  21. Tretyak B, Makukh H, Kitsera N. et al. The molecular genetic analysis of common ATM gene mutations among patients with Ataxia-telagiectasiasuspection. Factors of experimental evolution of organisms. 2015; (16); 251-5.
  22.  
  23. Lo YL, Hsiao CF, Jou YS. Et al. ATM polymorphisms and risk of lung cancer among never smokers. Lung Cancer. 2010; 69 (2); 148-54. CrossRef PubMed
  24.  
  25. Wang Y, Yang H, Li H. et al. Association between X-ray repair cross complementing group 1 codon 399 and 194 polymorphisms and lung cancer risk: a meta-analysis. Cancer. 2009; (285); 134-40. PubMed
  26.  

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