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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(4): 66-73


I.V. Sorokina 1, M.S. Myroshnychenko 1, N.V. Kapustnyk 1, S.I. Danylchenko 2

  1. Kharkiv National Medical University, Kharkiv, Ukraine
  2. Petro Mohyla Black Sea National University, Mykolaiv, Ukraine


It was determined the features of mesenchymal (vimentin, smooth muscle actin, desmin) and epithelial (cytokeratins 18 and 19) markers expression in the kidneys, ureters and bladder of full-term 7 fetuses and 11 newborn of WAG rats that developed under physiological conditions. The degree of immunohistochemical reactions expression with the above monoclonal antibodies was assessed using a semiquantitative scale. The study established the structural elements in the kidneys, ureters and bladder that expressed these markers. It has been shown that with age in the kidneys the expression of vimentin in the capsule, nephrogenic zone, stroma by the fibroblastic series cells, in the renal corpuscles by the epithelial cells of the inner leaf of the Bowman capsule, the mesangial cells, the endothelial cells of the capillaries, in the vessels of the stroma by the endothelial cells, and its expression disappears in the tubules in the epithelial cells; in the ureters and bladder its expression increases by the fibroblastic series cells and endothelial cells of the vessels. The expression of smooth muscle actin and desmin in the kidneys decreases in myofibroblasts located in the capsule and stroma, and increases in mesangial cells of smooth muscle type and smooth muscle cells of stroma vessels; in the ureters and bladder decreases in myofibroblasts, increases in smooth muscle cells of the vessels and the muscle layer. Immunohistochemical reaction with cytokeratins 18 and 19 is more pronounced in the kidneys in tubular nephron apparatus, in the ureters and bladder in transitional epithelium of the mucous membranes. The revealed features of the vimentin, smooth muscle actin, desmin, cytokeratins 18 and 19 expression in the kidneys, ureters and bladder of WAG rats descendants that developed under physiological conditions can be used as normative parameters in conducting studies.

Keywords: WAG rats; fetus; newborn; kidney; ureter; bladder; vimentin; smooth muscle actin; desmin; cytokeratins 18 and 19.


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