IMMUNOHISTOCHEMICAL CHARACTERISTICS OF SOME MESENCHYMAL AND EPITHELIAL MARKERS EXPRESSION IN THE KIDNEYS, URETERS AND BLADDER OF WAG RATS DESCENDANTS THAT DEVELOPED UNDER PHYSIOLOGICAL CONDITIONS
I.V. Sorokina 1, M.S. Myroshnychenko 1, N.V. Kapustnyk 1, S.I. Danylchenko 2
- Kharkiv National Medical University, Kharkiv, Ukraine
- Petro Mohyla Black Sea National University, Mykolaiv, Ukraine
DOI: https://doi.org/10.15407/fz64.04.066
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Abstract
It was determined the features of mesenchymal (vimentin,
smooth muscle actin, desmin) and epithelial (cytokeratins
18 and 19) markers expression in the kidneys, ureters and
bladder of full-term 7 fetuses and 11 newborn of WAG rats
that developed under physiological conditions. The degree of
immunohistochemical reactions expression with the above
monoclonal antibodies was assessed using a semiquantitative
scale. The study established the structural elements in the
kidneys, ureters and bladder that expressed these markers. It
has been shown that with age in the kidneys the expression
of vimentin in the capsule, nephrogenic zone, stroma by the
fibroblastic series cells, in the renal corpuscles by the epithelial
cells of the inner leaf of the Bowman capsule, the mesangial
cells, the endothelial cells of the capillaries, in the vessels
of the stroma by the endothelial cells, and its expression
disappears in the tubules in the epithelial cells; in the ureters
and bladder its expression increases by the fibroblastic series
cells and endothelial cells of the vessels. The expression of
smooth muscle actin and desmin in the kidneys decreases
in myofibroblasts located in the capsule and stroma, and
increases in mesangial cells of smooth muscle type and smooth
muscle cells of stroma vessels; in the ureters and bladder
decreases in myofibroblasts, increases in smooth muscle cells
of the vessels and the muscle layer. Immunohistochemical
reaction with cytokeratins 18 and 19 is more pronounced in
the kidneys in tubular nephron apparatus, in the ureters and
bladder in transitional epithelium of the mucous membranes.
The revealed features of the vimentin, smooth muscle actin,
desmin, cytokeratins 18 and 19 expression in the kidneys,
ureters and bladder of WAG rats descendants that developed
under physiological conditions can be used as normative
parameters in conducting studies.
Keywords:
WAG rats; fetus; newborn; kidney; ureter; bladder; vimentin; smooth muscle actin; desmin; cytokeratins 18 and 19.
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