THE CHANGES OF MINERAL DENSITY OF DENTO-MAXILLARY SYSTEM OF RATS WITH IODINE DEFICIENCY AND INSULIN RESISTANCE
S.P. Guranych, N.M. Voronych-Semchenko, T.V. Guranych
Ivano-Frankivsk National Medical University, Ukraine
The changes of indexes of the activity of enzymes of bioenergetic
processes, macro- and microelement status of rats
with iodine deficiency and insulin resistance were analyzed. It
was found that the development of iodine deficiency was accompanied
by inhibition of lactate dehydrogenase (LDH) and
alkaline phosphatase (ALP) activity against the background
of increased activity of acid phosphatase (ACP), decreased
calcium and magnesium content in solid tissues of teeth and
bones in comparison with data in intact animals. The decreased
activity of succinate dehydrogenase (SDH) and LDH on the
background of increased activity of malate dehydrogenase
(MDH) and ACP, decreased calcium and magnesium content
in solid tissues of teeth and alveolar processes, increased zinc
content in the teeth under the conditions of insulin resistance. It
can be assumed that under the conditions of iodine deficiency,
the activity of osteoblasts was suppressed, while osteoclasts
were more activated in case of insulin resistance.
In rats with insulin resistance on the background of iodine
deficiency, a significant decrease of dehydrogenase activity in
blood serum (on 90.38-97.12 %) was observed as to indexes
in intact animals. In erythrocyte mass, teeth and alveolar
processes, the content of calcium, magnesium and manganese
decreased by 20.26-88.26 % with a simultaneous increase
in zinc content in dental tissues at 77.35 %. The violation
of calcium homeostasis was conformed with the increase in
the activity of ACP (2.42 times), that may be the cause of
increased demineralization of teeth and bones in conditions of
insulin resistance on the background of iodine deficiency. The
determination of the activity of bioenergy synthesis enzymes
(SDH, LDH) under conditions of endocrinopathy can be used
as one of the early markers of subcellular metabolic disorders
of macroenergy formation. The changes of the enzymatic
activity of ACP and ALP indicate the progression of bone tissue
regeneration processes, which allows to detect disturbances of
the mineralization of solid tissues on the early stages of iodine
deficiency and insulin resistance development.
iodine deficiency; insulin resistance; energy synthesis enzymes; macro- and microelement status.
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