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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(4): 41-50

INFLUENCE OF POLARIZED LIGHT ON VITALITY OF RETINAL GANGLION CELLS IN RATS WITH LOW-TENSION GLAUCOMA

K.S. Agashkov1, M.Y. Krasniakova1, N.S. Nikolaychuk2, О.A. Rybachuk1, E.Y. Zabenko1, A.V. Dromaretsky1, I.V. Shargorodskaya2, S.A. Rikov2, N.V. Voitenko1

  1. O.O.Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. National Medical Academy of Postgraduate Education, Ukraine
DOI: https://doi.org/10.15407/fz64.04.041

Abstract

In this study, low-tension glaucoma in rats was induced by damage to the optic nerves. Retinal ganglion cells were identified by the injection of the fluorescent dye Fluorogold into the superior colliculi of the midbrain - the structure where axons of these cells terminate. It was shown that in rats with experimental glaucoma the amount of retinal ganglion cells significantly decreased in comparison with control rats. The mean value of the number of cells in the retina obtained from rats with low-tension glaucoma was 235.2 ± 29.9, while the mean value of the number of cells in the control retinas was 567.0 ± 42.2. An analysis of the effect of polarized light on vitality of retinal ganglion cells in the retinas from control rats did not reveal any significant differences from the untreated ones. When comparing the amount of left and right eye retinal ganglion cells, there was no significant difference between two groups, although there was a positive tendency to maintain a greater amount of cells in retinal tissue when treated with polarized green light. The mean value of the number of cells in the control retinas, without the effect of polarized green light, was 567.0 ± 42.2, whereas the average value of the number of cells in the retina affected by polarized light was 646.3 ± 29.3. The results of this experiment show that the protocols of influence of polarized green light on the healthy eye are not toxic or harmful and can be used for further investigation in the establishment of therapeutic effects in the experimental model of glaucoma of rats. An analysis of the effect of polarized light on vitality of retinal ganglion cells in the model of experimental low-tension glaucoma in rats did not reveal any significant differences from the control.

Keywords: low-tension glaucoma; retinal ganglion cells; polarized light

Full text: (PDF, in Ukrainian)

References

  1. Quigley HA, Broman AT. The number of people with glaucoma worldwide in 2010 and 2020. Br J Ophthalmol. 2006;90(3):262-7. CrossRef PubMed PubMedCentral
    2.  
  2. Tielsch JM, Katz J, Singh K, Quigley HA, Gottsch JD, Javitt J, et al. A population-based evaluation of glaucoma screening: the Baltimore Eye Survey. Am J Epidemiol. 1991;134(10):1102-10. CrossRef PubMed
    3.  
  3. Bueno JM, Artal P. Polarization and retinal image quality estimates in the human eye. J Opt Soc Am A. 2001;18(3):489. CrossRef  
  4. Salinas-Navarro M, Mayor-Torroglosa S, Jiménez-López M, Avilés-Trigueros M, Holmes TM, Lund RD, et al. A computerized analysis of the entire retinal ganglion cell population and its spatial distribution in adult rats. Vision Res. 2009;49(1):115-26. CrossRef PubMed
    5.  
  5. Nadal-Nicolás FM, Salinas-Navarro M, Jiménez- LÃ3pez M, Sobrado-Calvo P, Villegas-Pérez MP, Vidal- Sanz M, et al. Displaced retinal ganglion cells in albino and pigmented rats. Front Neuroanat. 2014;8.
    6.  
  6. Association for Research in Vision and Ophthalmology. E, Zalish M, Lavie V, Duvdevani R, Ben-Bassat S, Schwartz M. Investigative ophthalmology & visual science. [Internet]. Vol. 33, Investigative Ophthalmology & Visual Science. C.V. 1977;3586-91.
    7.  
  7. Li Y, Zhang F, Nagai N, Tang Z, Zhang S, Scotney P, et al. VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats. J Clin Invest. 2008;118(3):913-23. CrossRef  
  8. Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, et al. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3β phosphorylation. J Exp Med. 2010;207(4):867-80. CrossRef PubMed PubMedCentral
    9.  
  9. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. Elsevier; 2007. PubMedCentral
    10.  
  10. Tang Z, Zhang S, Lee C, Kumar A, Arjunan P, Li Y, et al. An optic nerve crush injury murine model to study retinal ganglion cell survival. J Vis Exp. 201;(50). CrossRef PubMed PubMedCentral
    11.  
  11. Bouhenni R, Dunmire J, Sewell A, and Edward D. Animal Models of Glaucoma. J Biomed and Biotechnol. 2012; 2012(692609). CrossRef  
  12. Levin LA. Animal and culture models of glaucoma for studying neuroprotection. Eur J Ophthalmol. 2001;11(2):S23-S29. CrossRef PubMed
    13.  
  13. Carmignoto G, Maffei L, Candeo P, Canella R, Cornelli C. Effect of NGF on the Survival of Rat Retinal Ganglion Cells Following Optic Nerve Section. J Neurosci. 1989;(4):1263-72. CrossRef PubMed
    14.   Nair CE Mac, Schlamp CL, Montgomery AD, Shestopalov VI, Nickells RW. Retinal glial responses to optic nerve crush are attenuated in Bax-deficient mice and modulated by purinergic signaling pathways. Journal of Neuroinflammation. (2016) 13:93 CrossRef PubMed PubMedCentral  
  14. Qu L, Gao L, Xu H, Duan P, Zeng Y, Liu Y, et al. Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats. Sci Rep. 2017;7(1):199. CrossRef PubMed PubMedCentral
    15.  
  15. Sadun AA, Carelli V. The role of mitochondria in health, ageing, and diseases affecting vision. Br J Ophthalmol. 2006;90(7):809-10. CrossRef PubMed PubMedCentral
    16.  
  16. Mikheytseva IN, Kashintseva LT, Artemov AV, Khramenko NI. Study of corvitin efficiency inprimery glaucoma. Fiziol Zh. 2012;58(6), 81-8.
    17.  
  17. Mikheytseva IN. Protective action of melatonine in experimental glaucoma in rats. Fiziol Zh. 2013;59(1):78-83.
    18.  
  18. Mikheytseva IN, Siroshtanenko TI. Effect of hydrogen sulphide donor on intraocular pressure in rats. Fiziol Zh. 2016;62(5):57-61. CrossRef  
  19. Kalesnykas G, Oglesby EN, Zack DJ, Cone FE, Steinhart MR, Tian J, et al. Retinal ganglion cell morphology after optic nerve crush and experimental glaucoma. Invest Ophthalmol Vis Sci. 2012;53(7):3847-57. CrossRef PubMed PubMedCentral
    20.  
  20. Karu TI, Pyatibrat L V., Kolyakov SF, Afanasyeva NI. Absorption measurements of a cell monolayer relevant to phototherapy: Reduction of cytochrome c oxidase under near IR radiation. J Photochem Photobiol B Biol. 2005;81(2):98-106. CrossRef PubMed
    21.  
  21. Wong-Riley MTT, Liang HL, Eells JT, Chance B, Henry MM, Buchmann E, et al. Photobiomodulation Directly Benefits Primary Neurons Functionally Inactivated by Toxins. J Biol Chem. 2005;280(6):4761-71. CrossRef PubMed
    22.  
  22. Eells JT, Henry MM, Summerfelt P, Wong-Riley MTT, Buchmann E V., Kane M, et al. Therapeutic photobiomodulation for methanol-induced retinal toxicity. Proc Natl Acad Sci. 2003;100(6):3439-44. CrossRef PubMed PubMedCentral
    23.  
  23. Karu T. Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B Biol. 1999;49(1):1-17. CrossRef  
  24. Lim J, Gattone VH, Sinders R, Miller CA, Liang Y, Harris P, et al. Acceleration of the meckel syndrome by nearinfrared light therapy. Nephron Extra. 2011;1(1):224-34. CrossRef PubMed PubMedCentral
    25.  
  25. Wong-Riley MTT. Bigenomic regulation of cytochrome c oxidase in neurons and the tight coupling between neuronal activity and energy metabolism. Adv Exp Med Biol. 2012;748:283-304. CrossRef PubMed PubMedCentral
    26.  
  26. Surendranath P, Arjunkumar R. Low Level Laser Therapy -A Review. IOSR J Dent Med Sci. 1989;12(5):2279-861.
    27.  

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