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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

(English title: Physiological Journal)

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(4): 41-50


INFLUENCE OF POLARIZED LIGHT ON VITALITY OF RETINAL GANGLION CELLS IN RATS WITH LOW-TENSION GLAUCOMA

K.S. Agashkov1, M.Y. Krasniakova1, N.S. Nikolaychuk2, О.A. Rybachuk1, E.Y. Zabenko1, A.V. Dromaretsky1, I.V. Shargorodskaya2, S.A. Rikov2, N.V. Voitenko1

  1. O.O.Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. National Medical Academy of Postgraduate Education, Ukraine
DOI: https://doi.org/10.15407/fz64.04.041


Abstract

In this study, low-tension glaucoma in rats was induced by damage to the optic nerves. Retinal ganglion cells were identified by the injection of the fluorescent dye Fluorogold into the superior colliculi of the midbrain - the structure where axons of these cells terminate. It was shown that in rats with experimental glaucoma the amount of retinal ganglion cells significantly decreased in comparison with control rats. The mean value of the number of cells in the retina obtained from rats with low-tension glaucoma was 235.2 ± 29.9, while the mean value of the number of cells in the control retinas was 567.0 ± 42.2. An analysis of the effect of polarized light on vitality of retinal ganglion cells in the retinas from control rats did not reveal any significant differences from the untreated ones. When comparing the amount of left and right eye retinal ganglion cells, there was no significant difference between two groups, although there was a positive tendency to maintain a greater amount of cells in retinal tissue when treated with polarized green light. The mean value of the number of cells in the control retinas, without the effect of polarized green light, was 567.0 ± 42.2, whereas the average value of the number of cells in the retina affected by polarized light was 646.3 ± 29.3. The results of this experiment show that the protocols of influence of polarized green light on the healthy eye are not toxic or harmful and can be used for further investigation in the establishment of therapeutic effects in the experimental model of glaucoma of rats. An analysis of the effect of polarized light on vitality of retinal ganglion cells in the model of experimental low-tension glaucoma in rats did not reveal any significant differences from the control.

Keywords: low-tension glaucoma; retinal ganglion cells; polarized light

References

  1. Quigley HA, Broman AT. The number of people with glaucoma worldwide in 2010 and 2020. Br J Ophthalmol. 2006;90(3):262-7. CrossRef PubMed PubMedCentral
  2.  
  3. Tielsch JM, Katz J, Singh K, Quigley HA, Gottsch JD, Javitt J, et al. A population-based evaluation of glaucoma screening: the Baltimore Eye Survey. Am J Epidemiol. 1991;134(10):1102-10. CrossRef PubMed
  4.  
  5. Bueno JM, Artal P. Polarization and retinal image quality estimates in the human eye. J Opt Soc Am A. 2001;18(3):489. CrossRef
  6.  
  7. Salinas-Navarro M, Mayor-Torroglosa S, Jiménez-López M, Avilés-Trigueros M, Holmes TM, Lund RD, et al. A computerized analysis of the entire retinal ganglion cell population and its spatial distribution in adult rats. Vision Res. 2009;49(1):115-26. CrossRef PubMed
  8.  
  9. Nadal-Nicolás FM, Salinas-Navarro M, Jiménez- LÃ3pez M, Sobrado-Calvo P, Villegas-Pérez MP, Vidal- Sanz M, et al. Displaced retinal ganglion cells in albino and pigmented rats. Front Neuroanat. 2014;8.
  10.  
  11. Association for Research in Vision and Ophthalmology. E, Zalish M, Lavie V, Duvdevani R, Ben-Bassat S, Schwartz M. Investigative ophthalmology & visual science. [Internet]. Vol. 33, Investigative Ophthalmology & Visual Science. C.V. 1977;3586-91.
  12.  
  13. Li Y, Zhang F, Nagai N, Tang Z, Zhang S, Scotney P, et al. VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats. J Clin Invest. 2008;118(3):913-23. CrossRef
  14.  
  15. Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, et al. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3β phosphorylation. J Exp Med. 2010;207(4):867-80. CrossRef PubMed PubMedCentral
  16.  
  17. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. Elsevier; 2007. PubMedCentral
  18.  
  19. Tang Z, Zhang S, Lee C, Kumar A, Arjunan P, Li Y, et al. An optic nerve crush injury murine model to study retinal ganglion cell survival. J Vis Exp. 201;(50). CrossRef PubMed PubMedCentral
  20.  
  21. Bouhenni R, Dunmire J, Sewell A, and Edward D. Animal Models of Glaucoma. J Biomed and Biotechnol. 2012; 2012(692609). CrossRef
  22.  
  23. Levin LA. Animal and culture models of glaucoma for studying neuroprotection. Eur J Ophthalmol. 2001;11(2):S23-S29. CrossRef PubMed
  24.  
  25. Carmignoto G, Maffei L, Candeo P, Canella R, Cornelli C. Effect of NGF on the Survival of Rat Retinal Ganglion Cells Following Optic Nerve Section. J Neurosci. 1989;(4):1263-72. CrossRef PubMed
  26.   Nair CE Mac, Schlamp CL, Montgomery AD, Shestopalov VI, Nickells RW. Retinal glial responses to optic nerve crush are attenuated in Bax-deficient mice and modulated by purinergic signaling pathways. Journal of Neuroinflammation. (2016) 13:93 CrossRef PubMed PubMedCentral  
  27. Qu L, Gao L, Xu H, Duan P, Zeng Y, Liu Y, et al. Combined transplantation of human mesenchymal stem cells and human retinal progenitor cells into the subretinal space of RCS rats. Sci Rep. 2017;7(1):199. CrossRef PubMed PubMedCentral
  28.  
  29. Sadun AA, Carelli V. The role of mitochondria in health, ageing, and diseases affecting vision. Br J Ophthalmol. 2006;90(7):809-10. CrossRef PubMed PubMedCentral
  30.  
  31. Mikheytseva IN, Kashintseva LT, Artemov AV, Khramenko NI. Study of corvitin efficiency inprimery glaucoma. Fiziol Zh. 2012;58(6), 81-8.
  32.  
  33. Mikheytseva IN. Protective action of melatonine in experimental glaucoma in rats. Fiziol Zh. 2013;59(1):78-83.
  34.  
  35. Mikheytseva IN, Siroshtanenko TI. Effect of hydrogen sulphide donor on intraocular pressure in rats. Fiziol Zh. 2016;62(5):57-61. CrossRef
  36.  
  37. Kalesnykas G, Oglesby EN, Zack DJ, Cone FE, Steinhart MR, Tian J, et al. Retinal ganglion cell morphology after optic nerve crush and experimental glaucoma. Invest Ophthalmol Vis Sci. 2012;53(7):3847-57. CrossRef PubMed PubMedCentral
  38.  
  39. Karu TI, Pyatibrat L V., Kolyakov SF, Afanasyeva NI. Absorption measurements of a cell monolayer relevant to phototherapy: Reduction of cytochrome c oxidase under near IR radiation. J Photochem Photobiol B Biol. 2005;81(2):98-106. CrossRef PubMed
  40.  
  41. Wong-Riley MTT, Liang HL, Eells JT, Chance B, Henry MM, Buchmann E, et al. Photobiomodulation Directly Benefits Primary Neurons Functionally Inactivated by Toxins. J Biol Chem. 2005;280(6):4761-71. CrossRef PubMed
  42.  
  43. Eells JT, Henry MM, Summerfelt P, Wong-Riley MTT, Buchmann E V., Kane M, et al. Therapeutic photobiomodulation for methanol-induced retinal toxicity. Proc Natl Acad Sci. 2003;100(6):3439-44. CrossRef PubMed PubMedCentral
  44.  
  45. Karu T. Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B Biol. 1999;49(1):1-17. CrossRef.1016/S1011-1344(98)00219-X
  46.  
  47. Lim J, Gattone VH, Sinders R, Miller CA, Liang Y, Harris P, et al. Acceleration of the meckel syndrome by nearinfrared light therapy. Nephron Extra. 2011;1(1):224-34. CrossRef PubMed PubMedCentral
  48.  
  49. Wong-Riley MTT. Bigenomic regulation of cytochrome c oxidase in neurons and the tight coupling between neuronal activity and energy metabolism. Adv Exp Med Biol. 2012;748:283-304. CrossRef.1007/978-1-4614-3573-0_12 PubMed PubMedCentral
  50.  
  51. Surendranath P, Arjunkumar R. Low Level Laser Therapy -A Review. IOSR J Dent Med Sci. 1989;12(5):2279-861.
  52.  

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