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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(4): 41-50


INFLUENCE OF POLARIZED LIGHT ON VITALITY OF RETINAL GANGLION CELLS IN RATS WITH LOW-TENSION GLAUCOMA

K.S. Agashkov1, M.Y. Krasniakova1, N.S. Nikolaychuk2, О.A. Rybachuk1, E.Y. Zabenko1, A.V. Dromaretsky1, I.V. Shargorodskaya2, S.A. Rikov2, N.V. Voitenko1

  1. O.O.Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. National Medical Academy of Postgraduate Education, Ukraine
DOI: https://doi.org/10.15407/fz64.04.041

Abstract

In this study, low-tension glaucoma in rats was induced by damage to the optic nerves. Retinal ganglion cells were identified by the injection of the fluorescent dye Fluorogold into the superior colliculi of the midbrain - the structure where axons of these cells terminate. It was shown that in rats with experimental glaucoma the amount of retinal ganglion cells significantly decreased in comparison with control rats. The mean value of the number of cells in the retina obtained from rats with low-tension glaucoma was 235.2 ± 29.9, while the mean value of the number of cells in the control retinas was 567.0 ± 42.2. An analysis of the effect of polarized light on vitality of retinal ganglion cells in the retinas from control rats did not reveal any significant differences from the untreated ones. When comparing the amount of left and right eye retinal ganglion cells, there was no significant difference between two groups, although there was a positive tendency to maintain a greater amount of cells in retinal tissue when treated with polarized green light. The mean value of the number of cells in the control retinas, without the effect of polarized green light, was 567.0 ± 42.2, whereas the average value of the number of cells in the retina affected by polarized light was 646.3 ± 29.3. The results of this experiment show that the protocols of influence of polarized green light on the healthy eye are not toxic or harmful and can be used for further investigation in the establishment of therapeutic effects in the experimental model of glaucoma of rats. An analysis of the effect of polarized light on vitality of retinal ganglion cells in the model of experimental low-tension glaucoma in rats did not reveal any significant differences from the control.

Keywords: low-tension glaucoma; retinal ganglion cells; polarized light

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