INFLUENCE OF POLARIZED LIGHT ON VITALITY OF RETINAL GANGLION CELLS IN RATS WITH LOW-TENSION GLAUCOMA
K.S. Agashkov1, M.Y. Krasniakova1, N.S. Nikolaychuk2, О.A. Rybachuk1, E.Y. Zabenko1, A.V. Dromaretsky1, I.V. Shargorodskaya2, S.A. Rikov2, N.V. Voitenko1
- O.O.Bogomoletz Institute of Physiology of the National
Academy of Sciences of Ukraine, Kyiv, Ukraine
- National Medical Academy of Postgraduate Education, Ukraine
DOI: https://doi.org/10.15407/fz64.04.041
Abstract
In this study, low-tension glaucoma in rats was induced by
damage to the optic nerves. Retinal ganglion cells were identified
by the injection of the fluorescent dye Fluorogold into
the superior colliculi of the midbrain - the structure where
axons of these cells terminate. It was shown that in rats with
experimental glaucoma the amount of retinal ganglion cells
significantly decreased in comparison with control rats. The
mean value of the number of cells in the retina obtained from
rats with low-tension glaucoma was 235.2 ± 29.9, while the
mean value of the number of cells in the control retinas was
567.0 ± 42.2. An analysis of the effect of polarized light on
vitality of retinal ganglion cells in the retinas from control rats
did not reveal any significant differences from the untreated
ones. When comparing the amount of left and right eye retinal
ganglion cells, there was no significant difference between two
groups, although there was a positive tendency to maintain
a greater amount of cells in retinal tissue when treated with
polarized green light. The mean value of the number of cells in
the control retinas, without the effect of polarized green light,
was 567.0 ± 42.2, whereas the average value of the number
of cells in the retina affected by polarized light was 646.3 ±
29.3. The results of this experiment show that the protocols of
influence of polarized green light on the healthy eye are not
toxic or harmful and can be used for further investigation in
the establishment of therapeutic effects in the experimental
model of glaucoma of rats. An analysis of the effect of polarized
light on vitality of retinal ganglion cells in the model of
experimental low-tension glaucoma in rats did not reveal any
significant differences from the control.
Keywords:
low-tension glaucoma; retinal ganglion cells; polarized light
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