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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(4): 3-11


EFFECTS OF MULTIPOTENT STROMAL CELL TRANSPLANTATION ON MICE IMMUNE SYSTEM UNDER CONDITIONS OF ITS REGENERATION

I.S. Nikolsky1, V.V. Nikolskaya1, D.L. Demchenko1, L.I. Taranukha1, Y.-M.А.Semenova1, T.V.Serebrovska2

  1. State Institute of Genetic and Regenerative Medicine National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
  2. O.O.Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.04.003

Abstract

The effects of thymic multipotent stromal cells (MSC) transplantation on the regeneration of mice immune system damaged by cyclophosphamide was investigated. Three groups of mice were examined: immunized control animals, immunized mice receiving cyclophosphamide, and immunized animals receiving cyclophosphamide and MSC. A single intraperitoneal injection of cyclophosphamide was performed at a dose of 200 mg / kg. Two hours later, thymic MSCs (5×104, 0.1 ml) were injected into the retroorbital sinus. One week after MSC administration the mice of all groups were immunized with sheep erythrocytes (108), after 4 days a similar number of cells were re-injected into the hind foot pad, and the immune system was assessed after 24 hours. It was shown that transplantation of MSC led to a significant decrease in granulocyte count (by 25%), hematocrit (by 8.1%) and the number of reticulocytes (by 71.4%), however, expressed stimulation of the regenerative activity of the immune system was registered. The effect of MSC on innate and adaptive immunity was characterized by a marked increase in the natural cytotoxicity of splenocytes (by 2.7 times) and the phagocytic index of peritoneal macrophages (by 72.2%), normalization of bactericidal activity in the NCT test, and also expressed by stimulation of antibody response. The obtained results show that transplantation of thymic MSC to mice with the regeneration of hemoimunopoiesis after cyclophosphamide administration can manifest in multidirectional effects: stimulation of the immune system and delay in the renewal of granulocyte and erythrocyte germ cells. The data obtained can be used to develop methods for the targeted regeneration of the lymphoid component of the immune system, enhancement of natural immunity and a humoral immune response.

Keywords: thymus multipotent stromal cells; immune system regeneration; cyclophosphamide heme immunodeficiency.

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