THE FEATURES OF CELLULAR COMPOSITION OF RAT BONE MARROW AT ACUTE IRRADIATION AND S-HETERYL MODIFIED CYSTEAMINE ADMINISTRATION
N.E. Uzlenkova1, N.G. Skorobogatova1, N.N. Moiseyeva2, E.L. Maslennikova1
- SI “Grigoriev Institute for Medical Radiology of National
Academy of Medical Sciences of Ukraine”, Kharkiv, Ukraine
- Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz64.03.061
Abstract
We studied the changes in the bone marrow (BM) cell
composition at administration of the compound S-heteryl
modified cysteamine (S-HMC) in the experiment with acute
in vivo irradiation. After total irradiation at a dose of 7,0 Gy
depletion of BM cell pool with a maximum at day 3 to 2,6 ±
0,9 % cells of control level was observed and changing the
ratios of early and differentiated elements of granulocytic,
lymphoid and erythroid lineages was detected. Intraperitoneal
administration of S-HMC (150 mg / kg) in 15 minutes before
irradiation resulted in an increase of the total myelokaryocytes
number by 2.2 times at day 3 and it led to normalization of this parameter on day 7 relatively to the irradiated control.
Recovery of granulocyto-, lympho- and erythropoiesis was
in shorter terms and it prevented the hematopoietic BM pool
depletion after irradiation. Under in vitro culture conditions
it was established a decrease in number of clonogenic mesenchymal
stromal cells (MSCs) in BM at day 3 after irradiation
to 29,3 ± 3,6 per femoral bone. It was determined survival
of a greater number (2.7 times) of the clonogenic BM MSCs
when S-HMC was administrated. Thus, the results indicate
the manifest myeloprotective effect of the S-HMC compound.
Keywords:
bone marrow; hematopoietic cells; mesenchymal stromal cells; irradiation; S-heteryl modified cysteamine
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