Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(3): 61-69

THE FEATURES OF CELLULAR COMPOSITION OF RAT BONE MARROW AT ACUTE IRRADIATION AND S-HETERYL MODIFIED CYSTEAMINE ADMINISTRATION

N.E. Uzlenkova1, N.G. Skorobogatova1, N.N. Moiseyeva2, E.L. Maslennikova1

  1. SI “Grigoriev Institute for Medical Radiology of National Academy of Medical Sciences of Ukraine”, Kharkiv, Ukraine
  2. Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz64.03.061


Abstract

We studied the changes in the bone marrow (BM) cell composition at administration of the compound S-heteryl modified cysteamine (S-HMC) in the experiment with acute in vivo irradiation. After total irradiation at a dose of 7,0 Gy depletion of BM cell pool with a maximum at day 3 to 2,6 ± 0,9 % cells of control level was observed and changing the ratios of early and differentiated elements of granulocytic, lymphoid and erythroid lineages was detected. Intraperitoneal administration of S-HMC (150 mg / kg) in 15 minutes before irradiation resulted in an increase of the total myelokaryocytes number by 2.2 times at day 3 and it led to normalization of this parameter on day 7 relatively to the irradiated control. Recovery of granulocyto-, lympho- and erythropoiesis was in shorter terms and it prevented the hematopoietic BM pool depletion after irradiation. Under in vitro culture conditions it was established a decrease in number of clonogenic mesenchymal stromal cells (MSCs) in BM at day 3 after irradiation to 29,3 ± 3,6 per femoral bone. It was determined survival of a greater number (2.7 times) of the clonogenic BM MSCs when S-HMC was administrated. Thus, the results indicate the manifest myeloprotective effect of the S-HMC compound.

Keywords: bone marrow; hematopoietic cells; mesenchymal stromal cells; irradiation; S-heteryl modified cysteamine

Full text: (PDF, in Ukrainian)

References

  1. Shao L, Luo Y, Zhou D. Hematopoietic stem cell injury induced by ionizing radiation. Antioxid Redox Signal. 2014; 20(9):1447-62. CrossRef PubMed PubMedCentral
    2.  
  2. Alessio N, Gaudio SD, Capasso S, Di Bernardo G, Cappabianca S, Cipollaro M, et al. Low dose radiation induced senescence of human mesenchymal stromal cells and impaired the autophagy process. Oncotarget. 2014;6(10):8155-66. PubMedCentral
    3.  
  3. Chen MF, Lin CT, Chen WC, Yang CT, Chen CC, Liao SK, et al. The sensitivity of human mesenchymal stem cells to ionizing radiation. Int J Radiat Oncol. Biol Phys. 2006;66(? 1):244-53.
    4.  
  4. Nicolay NH, Sommer E, Lopez R, Wirkner U, Trinh T, Sisombath S, et al. Mesenchymal stem cells retain their defining stem cell characteristics after exposure to ionizing radiation. Int J Radiat Oncol Biol Phys. 2013;87(5):1171-78. CrossRef PubMed
    5.  
  5. Ramdas J, Warrier RP, Scher C, Larussa V. Effects of Amifostine on Clonogenic Mesenchymal Progenitors and Hematopoietic Progenitors Exposed to Radiation. J Pediatr Hematol/Oncol. 2003 Jan 25(1):19-26. CrossRef PubMed
    6.  
  6. Okic-Djordjevic I, Trivanovic D, Iovanovic M, Ignatovic M, Secerov B, Mojovic M, et al. Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector. Croat Med. J. 2014;55:45-9. CrossRef PubMed PubMedCentral
    7.  
  7. Uzlenkova NE, Brazhko OA, Kornet MM, Pasiuga VN, Skorobogatova NG, Leonova IA, et al. The experimental study of the potential radioprotectors – S(azaheteryl) sustituated cysteamine. Ukr J Radiol. 2014;2:149-54. [Ukranian].
    8.  
  8. Uzlenkova NE, Skorobogatova NG, Magda IY, Leonova IA, Nenyukova EV, Maslennikova EL. Efficiency of Sheterylmodified cysteamine at acute radiation syndrome in rats. Ukr J Radiol. 2017;25(1):37-44. [Ukranian].
    9.  
  9. Friedenstein AJa, Luria EA. Cellular Bases of Hemopoietic Microenvironment. 1-st ed. Moskow: Meditsina; 1980. [Russian].
    10.  
  10. Davis JM. Basic Cell Culture. Practical approach. 2-nd ed. Oxford: University Press; 2001.
    11.  
  11. Dainiak N, Gent RN, Carr Z, Schneider R, Bader J, Buglova E, et al. First global consensus for evidence-based management of the hematopoietic syndrome resulting from exposure to ionizing radiation. Disaster Med Public Health Prep. 2011;5(3):202-12. CrossRef PubMed PubMedCentral
    12.  
  12. Nikolskaya EI, Butenko GM. Structural-functional organization of the bone marrow hematopoietic stem cells niches. Klitin ta Organ Transplantol. 2016;4(1):101-17. [Russian].
    13.  
  13. Islam MS, Steming ME, Takahashi Y, Hui SK. Radiation response of mesenchymal stem cells derived from bone marrow and human pluripotent stem cells. J Radiation Res. 2015;56:269-77. CrossRef PubMed PubMedCentral
    14.  
  14. Wang Y, Zhu G, Wang J, Chen J. Irradiation alter the differentiation potential of bone marrow mesenchymal stem cells. Mol Med Rep. 2016;13(1):213-23. CrossRef PubMed PubMedCentral
    15.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.