THE FEATURES OF CELLULAR COMPOSITION OF RAT BONE MARROW AT ACUTE IRRADIATION AND S-HETERYL MODIFIED CYSTEAMINE ADMINISTRATION
N.E. Uzlenkova1, N.G. Skorobogatova1, N.N. Moiseyeva2, E.L. Maslennikova1
- SI “Grigoriev Institute for Medical Radiology of National
Academy of Medical Sciences of Ukraine”, Kharkiv, Ukraine
- Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine
We studied the changes in the bone marrow (BM) cell
composition at administration of the compound S-heteryl
modified cysteamine (S-HMC) in the experiment with acute
in vivo irradiation. After total irradiation at a dose of 7,0 Gy
depletion of BM cell pool with a maximum at day 3 to 2,6 ±
0,9 % cells of control level was observed and changing the
ratios of early and differentiated elements of granulocytic,
lymphoid and erythroid lineages was detected. Intraperitoneal
administration of S-HMC (150 mg / kg) in 15 minutes before
irradiation resulted in an increase of the total myelokaryocytes
number by 2.2 times at day 3 and it led to normalization of this parameter on day 7 relatively to the irradiated control.
Recovery of granulocyto-, lympho- and erythropoiesis was
in shorter terms and it prevented the hematopoietic BM pool
depletion after irradiation. Under in vitro culture conditions
it was established a decrease in number of clonogenic mesenchymal
stromal cells (MSCs) in BM at day 3 after irradiation
to 29,3 ± 3,6 per femoral bone. It was determined survival
of a greater number (2.7 times) of the clonogenic BM MSCs
when S-HMC was administrated. Thus, the results indicate
the manifest myeloprotective effect of the S-HMC compound.
bone marrow; hematopoietic cells; mesenchymal stromal cells; irradiation; S-heteryl modified cysteamine
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