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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(3): 61-69


THE FEATURES OF CELLULAR COMPOSITION OF RAT BONE MARROW AT ACUTE IRRADIATION AND S-HETERYL MODIFIED CYSTEAMINE ADMINISTRATION

N.E. Uzlenkova1, N.G. Skorobogatova1, N.N. Moiseyeva2, E.L. Maslennikova1

  1. SI “Grigoriev Institute for Medical Radiology of National Academy of Medical Sciences of Ukraine”, Kharkiv, Ukraine
  2. Institute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz64.03.061

Abstract

We studied the changes in the bone marrow (BM) cell composition at administration of the compound S-heteryl modified cysteamine (S-HMC) in the experiment with acute in vivo irradiation. After total irradiation at a dose of 7,0 Gy depletion of BM cell pool with a maximum at day 3 to 2,6 ± 0,9 % cells of control level was observed and changing the ratios of early and differentiated elements of granulocytic, lymphoid and erythroid lineages was detected. Intraperitoneal administration of S-HMC (150 mg / kg) in 15 minutes before irradiation resulted in an increase of the total myelokaryocytes number by 2.2 times at day 3 and it led to normalization of this parameter on day 7 relatively to the irradiated control. Recovery of granulocyto-, lympho- and erythropoiesis was in shorter terms and it prevented the hematopoietic BM pool depletion after irradiation. Under in vitro culture conditions it was established a decrease in number of clonogenic mesenchymal stromal cells (MSCs) in BM at day 3 after irradiation to 29,3 ± 3,6 per femoral bone. It was determined survival of a greater number (2.7 times) of the clonogenic BM MSCs when S-HMC was administrated. Thus, the results indicate the manifest myeloprotective effect of the S-HMC compound.

Keywords: bone marrow; hematopoietic cells; mesenchymal stromal cells; irradiation; S-heteryl modified cysteamine

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