Functional renal state of mature rats with a quick type of acetylation under conditions of cadmium chloride and sodium nitrate
T.M. Boychuk, T.I. Kmet, N.D. Filipets, L.I. Vlasyk, T.I. Hrachova
Higher State Educational Establishment of Ukraine «Bukovinian State Medical University»,
The experiments conducted on laboratory non-linear albino mature male rats with a quick type of acetylation
examined renal function under conditions of cadmium-nitrate intoxication. A combined introduction
of cadmium chloride (0.1 mg/kg intraperitoneally) and sodium nitrate (500 mg/kg intragastrically) during
14 days was found to result in 31.2 % increase of glomerular filtration rate (GFR). Due to increased GFR
volume regulating, excretory kidney functions are activated that was assessed by endogenic creatinine clearance.
In case of lack of hypercreatininemia the concentration of creatinine in the urine 16.5 % increased,
creatinine excretion became 31.8 % higher. Concentration of sodium ions in the urine 67.8 % decreased,
and excretion with urine decreased three times as much. Concentration of sodium ions in the blood plasma
6.6 % decreased. Al though renal regulation of sodium ions metabolism was not disturbed: distal reabsorption
did not change, proximal reabsorption 24.4 % increased. Retention of tubular mechanisms of sodium
metabolism regulation and glomerular-tubular balance are indicative of adaptive possibilities of the kidneys
of mature rats with a quick type of acetylation ensuring maintenance of water-electrolytic balance under
conditions of subacute cadmium-nitrate effect. Further investigations of the effect of a combined action of
cadmium chloride and sodium nitrate on mature rats with slow acetylation and comparative analysis of
the renal function are of great importance in order to assess a prognostic value of acetylation phenotype
in the development of nephropathy
quick acetylizers; cadmium chloride; sodium nitrate; kidneys
- Kravchenko IE, Fazylov VCh, Zaripova RG. Metabolic enzyme systems activity evaluation in patients with streptococcal tonsillitis and ways of pharmacologic correction with ksimedon. Bull Contempor Clin Med. 2013; 6 (3): 8-12. [Russian].
- Khalili H, Dashti-Khavidaki S, Amini M, Mahjub R, Hajiabdolbaghi M. Is there any difference between acetylator phenotypes in tuberculosis patients and healthy subjects? Eur J Clin Pharmacol. 2010; 66(3): 261-7.
- Pons D, deVries FR, van den Elsen PJ, Heijmans BT, Quax PH, Jukema JW. Epigenetic histone 37 acetylation modifiers in vascular remodelling: new targets for therapy in cardiovascular disease. Eur Heart J. 2009; 30(3): 266-77.
- Wang Q, Zhang Y, Yang C, Xiong H, Lin Y, Yao J, Li H, Xie L, Zhao W, Yao Y, Ning ZB, Zeng R, Xiong Y, Guan KL, Zhao S, Zhao GP. Acetylation of metabolic enzymes 31 coordinates carbon source utilization and metabolic flux. Science. 2010; 327(5968): 1004-7.
- Boychuk TN, Gordienko VV, Rohovoy YuYe. Chronorhythms of the kidneys: the age-old aspect under the conditions of metallotoksikoza: a monograph. Chernivtsi: Medical University. 2016; 179. [Ukrainian].
- Boychuk TN, Rohovoy YuYe, Popovich GB. Pathophysiology of hepatorenal syndrome in hemic hypoxia. Chernivtsi: Medical University. 2012; 192. [Ukrainian].
- Gozhenko AI, Dolomatov SI, Dolatova EA, Karchauskas VY, Pychteyev DM. Function of the kidneys with cadmium nephropathy under conditions of water and salt loading. Nephrology. 2002; 6 (3): 75-8. [Russian].
- Filipets ND, Sirman VM, Gozhenko AI. The mechanisms of ion regulation kidneys function in histohemic hypoxia and possible ways of its correction. Nephrology. 2014; 18(4): 57-1. [Russian].
- Filipets ND, Gozhenko AI, Sirman VM. Effect of modulators of ion channels on renal function at the initial stage of development of histohemic hypoxia. J NAMS Ukraine. 2014; 20(4): 483-7. [Ukrainian].
- Pershin GN. Methods of experimental chemotherapy (practical guide) / ed. GN. Pershina: 2nd edition. Moscow: Medicine, 1971; 453-7. [Russian].
- Berkhin EB., Ivanov YuI. Methods of experimental study of the kidneys and water-salt metabolism. Barnaul: Altai Book, Publishing House, 1972; 199. [Russian].
- Ryabov SI, Natochin YuV. Functional Nephrology. SPb.: Lan, 1997; 304. [Russian].
- Bernard A. Confusion about Cadmium Risks: The Unrecognized Limitations of an Extrapolated Paradigm. Environm Health Perspectiv. 2016; 124(1): 1-5.
- Kmet TI, Vlasyk LI. Dynamics of changes of behavioral reactions in albino rats of juvenile age with a diverse types of acetylation under conditions of a subacute effect of cadmium chloride and sodium nitrate. Buk Med Herald. 2003; 7(3): 111-4. [Ukrainian].
- Peng Q, Bakulski KM, Nan B, Park SK. Cadmium and Alzheimer's disease mortality in U.S. adults: Updated evidence with a urinary biomarker and extended followup time. Environ Res. 2017; 157: 44-1.
- Soblirova ZnKh, Kharina EA. Is Rapid Acetylation a Predictor of Kidney and Urinary Tract Diseases. Nephrol and Dialysis. 1991; 1(1): 20-3. [Russian].
- Kmet TI. Hygienic meaning of combined effect of sodium nitrate and cadmium chloride with taking into consideration age peculiarity and metabolism character. The Dissertation submitted for the Candidate of Sciences (Medicine) Degreet. Kyiv, 2006; 20. [Ukrainian].
- Gozhenko AI, Kravchuk AV, Nikitenko OP, Moskolenko OM, Sirman VM. Functional Renal Reserve. Odessa: Fenix. 2015; 180. [Ukrainian].
- Gozhenko AI., Filipets ND. The functional state of kidneys after adenosine triphosphate-sensitive potassium channels activation in experimental acute hypoxia. Fiziol Zh. 2014; 60(4): 22-9. [Ukrainian].