Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(2): 54-64


THE STUDY OF THE INITIAL STAGES OF ATHEROGENESIS AGAINST HIGH-FAT DIET

N.S. Turchina, S.I. Savosko

    Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.02.054

Abstract

The sings of initial atherogenesis against high-fat diet were described in article. he proposed diet composition includes animal fat, margarine, cholesterol, calcium carbonate and mercazolil. The effect of high-fat diet on structural changes in the liver, heart, aorta and brain of mice were studied. According to the results of the histological study, the lipid drops in the subendothelial space in the myocardium vessels, the aorta bulb and lesser in the endocardium were identified in 28,8% of the animals after a 14-week fatty “atherogenic” diet. The results of ultrastructural study were showed a violation in the intima and muscular layer of the aorta in 100% of the samples. The changes in endothelial cells were characterized by vacuolation and degradation of mitochondria, organelles reduction, focal cells desquamation from the basement membrane into lumen. Lipid inclusion was detected in the interstitial space and cells of the subintimal space (mean diameter 600-1200 nm). The dead cells, mitochondrial swelling and cristolysis in the cytoplasm of smooth muscle cells were observed in the tunica media of the aorta. The hepatic steatosis with dystrophic changes in hepatocytes was revealed in the liver and the sings of focal inflammatory reactions around the vessels (infiltration of monocytes, lymphocytes, basophils) was identified in the brain. The established structural changes in the organs we were consider as the initiation of atherogenesis.

Keywords: atherogenic high-fat diet; cholesterol; aorta; heart; liver; brain; mouse

References

  1. Kennedy AJ, Ellacott KL, King VL, Hasty AH. Mouse models of the metabolic syndrome. Dis Model Mech. 2010 Mar-Apr;3(3-4):156-66. CrossRef PubMed PubMedCentral
  2.  
  3. Nishina PM, Lowe S, Verstuyft J, Naggert JK, Kuypers FA, Paigen B. Effects of dietary fats from animal and plant sources on diet-induced fatty streak lesions in C57BL/6J mice. J Lipid Res. 1993 Aug;34(8):1413-22. PubMed
  4.  
  5. Schreyer SA, Wilson DL, LeBoeuf RC. C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis. Atherosclerosis. 1998 Jan;136(1):17-24. CrossRef  
  6. Zhou AX, Tabas I. The UPR in atherosclerosis. Semin Immunopathol. 2013 May;35(3):321-32. CrossRef PubMed PubMedCentral
  7.  
  8. Getz GS, Reardon CA. Animal models of atherosclerosis. Arterioscler Thromb Vasc Biol. 2012 May;32(5):1104-15. CrossRef PubMed PubMedCentral
  9.  
  10. Gonzalez L, Yu P, Trigatti BL. Mouse models of coronary artery atherosclerosis. J Cardiovasc Disord. 2016;3:1021.
  11.  
  12. Zaragoza C, Gomez-Guerrero C, Martin-Ventura JL, Blanco-Colio L, Lavin B, Mallavia B, et al. Animal models of cardiovascular diseases. J Biomed Biotechnol. 2011;2011:497841. CrossRef PubMed PubMedCentral
  13.  
  14. Turchina NS, Savosko SI. Bogomolets mational medical university. Model of atherosclerosis damages of the aorta in mices. Patent Ukrainy ? 117425. 2017 Cher 26.
  15.  
  16. Turchina NS, Savosko SI. Bogomolets National medical university. The high-fat diet in mices with model of atherosclerosis damages of the aorta. Patenet Ukrainy ? 117423. 2017 Cher 26.
  17.  
  18. Rakov OV. Comparison characteristics of mercazolilum and thyrozol in treatment of patients with diffuse toxic goiter. Endocrinol. 2016;21(2):137-42.
  19.  
  20. Cooper DS. Antithyroid drugs. N Engl J Med. 2005 Mar 3;352(9):905-17. CrossRef PubMed
  21.  
  22. Melnyk N, Dovgan I, Savosko S. Sciatic nerve injury at intracerebral hemorrhage in internal capsule in rats. Modern Science-Moderní veda. 2016;(1):136-41.
  23.  
  24. Pellizzon MA. Diet-induced atherosclerosis/hypercholesterolemia in rodent models: Brief scientific literature review [Internet]. [cited 2018 Jan 30]. Available from:www. researchdiets.com/.../atherosclerosis_3%20page.pdf.
  25.  
  26. Renard C, Van Obberghen E. Role of diabetes in atherosclerotic pathogenesis. What have we learned from animal models? Diabetes Metab. 2006 Feb;32(1):15-29. CrossRef  
  27. Stary HC, Chandler AB, Dinsmore RE, Fuster V, Glagov S, Insull W, et al. A definition of advanced types of atherosclerotic lesions and a histological classification of atherosclerosis. A report from the Committee on Vascular Lesions of the Council on Arteriosclerosis, American Heart Association. Circulation. 1995 Sep 1;92(5):1355-74. CrossRef PubMed
  28.  
  29. Zhuhua Z, Zhiquan W, Zhen Y, Yixin N, Weiwei Z, Xiaoyong L, et al. A novel mice model of metabolic syndrome: the high-fat-high-fructose diet-fed ICR mice. Exp Anim. 2015;64(4):435-42. CrossRef PubMed PubMedCentral
  30.  
  31. Hassan HH, Denis M, Krimbou L, Marcil M, Genest J. Cellular cholesterol homeostasis in vascular endothelial cells. Can J Cardiol. 2006 Feb;22 Suppl B:35B-40B.
  32.  
  33. Savitskiy IV, Sliusar AA, Miastkovskaja IV. Multifactorial modeling of atherosclerosis in rats. J Educ Health Sport. 2016;6(3):233-40.
  34.  
  35. Gabriels K, Hoving S, Seemann I, Visser NL, Gijbels MJ, Pol JF, et al. Local heart irradiation of ApoE(-/-) mice induces microvascular and endocardial damage and accelerates coronary atherosclerosis. Radiother Oncol. 2012 Dec;105(3):358-64. CrossRef PubMed
  36.  
  37. Janssen CI, Jansen D, Mutsaers MP, Dederen PJ, Geenen B, Mulder MT, et al. The effect of a high-fat diet on brain plasticity, inflammation and cognition in female ApoE4- knockin and ApoE-knockout mice. PLoS One. 2016 May 12;11(5):e0155307. CrossRef PubMed PubMedCentral
  38.  
  39. Matsuzawa N, Takamura T, Kurita S, Misu H, Ota T, Ando H, et al. Lipid-induced oxidative stress causes steatohepatitis in mice fed an atherogenic diet. Hepatology. 2007 Nov;46(5):1392-403. CrossRef PubMed
  40.  
  41. Okada K, Warabi E, Sugimoto H, Horie M, Gotoh N, Tokushige K, et al. Deletion of Nrf2 leads to rapid progression of steatohepatitis in mice fed atherogenic plus high-fat diet. J Gastroenterol. 2013 May;48(5):620-32. CrossRef PubMed
  42.  
  43. Côté I, Ngo Sock ET, Lévy É, Lavoie JM. An atherogenic diet decreases liver FXR gene expression and causes severe hepatic steatosis and hepatic cholesterol accumulation: effect of endurance training. Eur J Nutr. 2013 Aug;52(5):1523-32. CrossRef PubMed
  44.  
  45. Van der Heijden RA, Sheedfar F, Morrison MC, Hommelberg PP, Kor D, Kloosterhuis NJ, et al. High-fat diet induced obesity primes inflammation in adipose tissue prior to liver in C57BL/6j mice. Aging. 2015 Apr;7(4):256-68. CrossRef PubMed PubMedCentral
  46.  
  47. VanSaun MN, Lee IK, Washington MK, Matrisian L, Gorden DL. High fat diet induced hepatic steatosis establishes a permissive microenvironment for colorectal metastases and promotes primary dysplasia in a murine model. Am J Pathol. 2009 Jul;175(1):355-64. CrossRef PubMed PubMedCentral
  48.  
  49. Nishina PM, Verstuyft J, Paigen B. Synthetic low and high fat diets for the study of atherosclerosis in the mouse. J Lipid Res. 1990 May;31(5):859-69. PubMed
  50.  
  51. Chen S, Shimada K, Zhang W, Huang G, Crother TR, Arditi M. IL-17A is proatherogenic in high-fat dietinduced and Chlamydia pneumoniae infection-accelerated atherosclerosis in mice. J Immunol. 2010 Nov 1;185(9):5619-27. CrossRef PubMed PubMedCentral
  52.  
  53. Ma Y, Wang W, Zhang J, Lu Y, Wu W, Yan H, et al. Hyperlipidemia and atherosclerotic lesion development in Ldlr-deficient mice on a long-term high-fat diet. PLoS One. 2012;7(4):e35835. CrossRef PubMed PubMedCentral
  54.  
  55. Skibo GG. Experimental ischemia of brain. Kyiv: Nauk. dumka; 2016.
  56.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2018.