THE STUDY OF THE INITIAL STAGES OF ATHEROGENESIS AGAINST HIGH-FAT DIET
N.S. Turchina, S.I. Savosko
Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.02.054
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Abstract
The sings of initial atherogenesis against high-fat diet were
described in article. he proposed diet composition includes
animal fat, margarine, cholesterol, calcium carbonate and
mercazolil. The effect of high-fat diet on structural changes in
the liver, heart, aorta and brain of mice were studied. According
to the results of the histological study, the lipid drops in the
subendothelial space in the myocardium vessels, the aorta bulb
and lesser in the endocardium were identified in 28,8% of the
animals after a 14-week fatty “atherogenic” diet. The results
of ultrastructural study were showed a violation in the intima
and muscular layer of the aorta in 100% of the samples. The
changes in endothelial cells were characterized by vacuolation
and degradation of mitochondria, organelles reduction, focal
cells desquamation from the basement membrane into lumen.
Lipid inclusion was detected in the interstitial space and cells of
the subintimal space (mean diameter 600-1200 nm). The dead
cells, mitochondrial swelling and cristolysis in the cytoplasm
of smooth muscle cells were observed in the tunica media of
the aorta. The hepatic steatosis with dystrophic changes in
hepatocytes was revealed in the liver and the sings of focal
inflammatory reactions around the vessels (infiltration of
monocytes, lymphocytes, basophils) was identified in the
brain. The established structural changes in the organs we
were consider as the initiation of atherogenesis.
Keywords:
atherogenic high-fat diet; cholesterol; aorta; heart; liver; brain; mouse
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