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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(2): 40-46


THE ROLE AND REGULATION OF PHOSPHOLIPASE D IN BRAIN INSULIN SIGNALING PATHWAY

N.A. Babenko, V.S. Kharchenko

    Research Institute of Biology of the V. N. Karazin Kharkiv National University, Kharkiv, Ukraine
DOI: https://doi.org/10.15407/fz64.02.040

Abstract

The role of phospholipase D as a positive modulator of glucose uptake activation by insulin in the neocortex of 3-month-old rats were studied by using specific inhibitors of signaling pathways of insulin (wortmannin, LY294002, halopemide, C6-ceramide). It was found that in the rat neocortex inhibitors decreased the insulin-induced accumulation of phosphatidylethanol from 163% in the control to 115% under the influence of wortmannin and 112% under the action of LY294002. Inhibition of insulin stimulated phospholipase D by phosphatidylinositol-3-kinase inhibitors (wortmannin and LY294002) indicated downstream regulation of phospholipase D by phosphatidylinositol-3- kinase. The suppression of this phospholipase by a specific inhibitor haloperimide is accompanied by a decrease in the amount of labeled glucose in the neocortical tissue from 154% in the control to 111% under the influence of the inhibitor. Inhibition of insulin-induced glucose uptake by specific inhibitor of phospholipase D halopemide points to the key role of the enzyme in the regulation of glucose uptake in the rat neocortex. In addition, phospholipase D-dependent insulin signaling and glucose uptake in the rat cerebral cortex are highly sensitive to intracellular ceramide content. The results obtained in this study provide that modulation of PLD activity, as well as ceramides content in the cells, can be a useful tool for manipulating the nerve tissue sensitivity to insulin action

Keywords: phosphatidylinositol-3-kinase; phospholipase D; insulin; ceramide; neocortex; rats

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