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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(2): 34-39

Circulating in the blood desquamated endotheliocytes at the diabetic nephropathy

A.I. Gozhenko, H.S. Kuznetsova, K.S. Kuznetsova, T.M. Byts, E.A. Gozhenko, N.O. Shevchenko

    State Enterprise “Ukrainian Research Institute of Transport Medicine, Ministry of Health of Ukraine”, Odessa, Ukraine


The aim of the study was to determine the role of endothelial dysfunction (ED) in the pathogenesis of diabetic nephropathy (DN) in patients with diabetes mellitus (DM) 1 and 2 type with a severe course (state of decompensation). DN was manifested by a decrease in GFR and urinary syndrome. In patients with diabetes, depending on the glomerular filtration rate (GFR), ED was estimated by the number of circulating desquamation endothelial cells (CECs) with the simultaneous determination of NO2 - and NO3 - metabolites. The indicators of the urinary syndrome don’t correlate with the degree of GFR reduction. The concentration of CECs in blood plasma in patients with GFR ≥ 90 ml.min-1.1.73 m-2 was 3053 ± 210.1 cells/ml. The level of endotheliocythemia in patients with GFR from 42 to 88 ml.min-1.1.73 m-2 was 22 % higher and amounted to 3700 ± 260.6 cells/ml. Therefore, in the patients with DN, the number of CECs in blood plasma increases.

Keywords: diabetes mellitus; endothelial dysfunction; diabetic nephropathy; circulating endothelial cells; glomerular filtration rate; vascular endothelium; nitric oxide


  1. Tronko ND, Mankovsky BN. The state program "Diabetes mellitus". Treatment and diagnosis. 2009; 1: 58-9. [Russian].
  3. Porush JM, Faubert PF. Hypertension, diabetes mellitus and nephropathy. London: Sience Preess. 2010; 9-12.
  5. Kuznetsova ES, Kuznetsova AS, Shukhtin VV, Gozhenko AI. Rarticular qualities of the renal osmoregulatory function in patients with type 2 diabetes. Ukr J Nephrol Dialysis. 2015; 4(49): 21-6. [Russian].
  7. Hill GS, Karoui KE, Karras A, Mandet C, Duong Van Huyen JP, Nochy D, Bruneval P. Focal segmental glomerulosclerosis plays a major role in the progression of IgA nephropathy. 2011 Mar; 79(6): 635-42. doi:10.1038/ ki.2010.466 PubMed PMID:21160460
  9. El Karoui K, Hill GS, Karras A, Moulonguet L, Caudwell V, Loupy A, Bruneval P, Jacquot C, Nochy D. Focal segmental glomerulosclerosis plays a major role in the progression of IgA nephropathy. II. Light microscopic and clinical studies. Kidney Int. 2011 Mar; 79(6):643-54 doi: 10.1038/ki.2010.460. CrossRef  
  10. Shamkhalova MS, Kurumova KO, Klefortova II, Sitkin II, Ilyin AV, Arbuzova MI, et al. Factors promoting development of renal tubulointerstitial lesions in patients with diabetes mellitus. Diabetes mellitus. 2010; 3: 134-41. [Russian].
  12. Editorial. Renal haemodynamics, vascular physiology, vascular pathology, experimental hypertension (W144- W188). Nephrol Dial Transplant. 1Jun 2003; 18 Suppl 4: 593-607.
  14. Fornoni A, Ijaz A, Tejada T, Lenz O. Role of inflammation in Diabetic Nephropathy. Curr Diab Rev. 2008; 4: 10-7. PubMed PMID: 18220690 CrossRef  
  15. Ferrera N. Vascular endothelial growth factor. Arterioscler Thromb Vasc Biol. 2009; 29: 789-91. DOI: 10.1210/ er.2003-0027.
  17. Kizub IV, Klymenko KI, Soloviev AI. [Protein kinase C participation in mechanisms of vascular tone abnormality in diabetes mellitus. Part 2]. Pharmacol Drug Toxicol. 2014; 2 (38): 12-7. [Russian].
  19. Kamp TJ, Hell JW. Regulation of Cardiac L-Type Calcium Channels by Protein Kinase A and Protein Kinase C. Circ Res, 2000. 87(12). 1095-102. CrossRef PubMed
  21. Gozhenko AI, Kuznetsova HS, Kuznetsova KS, Byts TN, Susla AB. Endothelial dysfunction in the pathogenesis of diabetes complications. The message I. Endothelial dysfunction: etiology, pathogenesis and diagnostic methods. Endocrinology. 2017; 22(2): 171-81. [Russian].
  23. Martens CR, Edwards DG. Peripheral vascular dysfunction in chronic kidney disease. Card Res Pract. 2011; 2011: 257-67
  25. Hladovec J, Prerovsky I, Stanek V, Fabian J. Circulating Endothelial Cells in Acute Myocardial Infarction and Angina Pectoris. Klin Wochenschr. 1978; 56(20): 1033-6. CrossRef PubMed
  27. Susla OB, Misula IR. inventors, State Higher Educational Institution Ternopil State Medical University named after I. Ya. Gorbachevsky, assignee. Method for determining the content of circulating endothelial cells in the blood plasma. Ukraine Patent 57841, MPK A61V10/00. 2010 Sep 20. [Ukranian].
  29. Gao Y, Liu C, Zhang X, Gao J, Yang C. Circulating Endothelial Cells as Potential Markers of Atherosclerosis. 2008 Nov; 35(5): 638-642 S0317167100009446.
  31. Stevens LA, Claybon MA, Schmid CH, Chen J, Horio M, Imai E, et al. Evaluation of the Chronic Kidney Disease : Epidemiology Collaboration equation for estimating the glomerular filtration rate in multiple ethnicities. Kidney Int. 2011 Mar; 79(5): 555-62. doi:10.1038/ki.2010.462 CrossRef  
  32. Gozhenko AI, Kuznetsova HS, Kuznetsova KS, Kuznetsova OM, Byts TM, Zukow W. Morpho-functional basis of endothelial dysfunction in diabetes mellitus. Journal of Education, Health and Sport. 2017; 7(6):516-24. eISSN 2391-8306. doi [Russian].
  34. Kobylyanskiy AG, Kuznetsova TV, Soboleva GN. Determination of nitric oxide in the serum and plasma of the human blood. Method of high-efficient liquid chromatography. Biomed Chem. 2003; 49(6): 597-603. [Russian].
  36. Gozhenko AI, Kuznetsova HS, Olenovych OA, Kuznetsov SH, Kuznetsova KS, Byts TM. The number of circulating endotheliocytes in the blood plasma of the patients with diabetes mellitus increases.. Pharmacologyonline. 2017.3, 23-6.

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