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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2001; 47(6): 15-23


The effect of psilocybe cubensis extracton hippocampal neurons in vitro

M. G. Moldavan, A. A. Grodzinskaya, E. F. Solomko, M. L. Lomberh,S. P. Wasser, V. M. Storozhuk

    A.A.Bogomoletz Institute of Physiology NationalAcademy of Sciences of Ukraine, Kiev;M.G.Kholodny Institute of Botany NationalAcademy of Sciences of Ukraine, Kiev


Abstract

The action of P.cubensis mushroom extract, containing psilocybin (PCB) and psilocin, on spike activity of hippocampal CA1 pyramidal neurons was studied in in vitro rat brain slices. In 38 (76 %) out of 50 investigated neurons spike activity was decreased, in 2 (4 %) cells it increased. There was no response 10 (20 %) neurons. Application of the extract caused short burst firing in 12 (24 %) neurons. All neurons showing inhibition during PCB-containing extract application, were also inhibited by serotonin (5-НТ). Usually inhibitory reaction did not last over 4 - 5 min upon 3 min extract application and could be prolonged up to 10-43 min up on serotonin application. Part of M. G. Moldavan, A. A. Grodzinskaya, E. F. Solomko et al. 22 ISSN 0201-8489 Фізіол. журн., 2001, Т. 47, № 6 neurons were inhibited by serotonin and did not react to extract application. Inhibitory reactions induced by extract application were blocked by ritanserin in half of the tested units and were induced due to activation of 5-НТ2 serotonin receptors. The extract suppressed excitative spike reactions caused by appli- cation of L-glutamic acid. It is concluded, that application of PCB-containing extract in most cases reduced spike activity in hippo- campal CA1 pyramidal neurons and suppressed gluta- mate transmission.

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