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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(2): 19-25


EFFECT OF Ω-3 POLYUNSATURATED FATTY ACIDS ON THE FUNCTIONAL ACTIVITY OF MONOOXYGENASE SYSTEM IN THE LIVER MICROSOMAL FRACTION OF TUMOR-BEARING RATS

O.V. Ketsa, M.M. Marchenko, I.O. Shmarakov

    Fedkovich Chernovtsy National University, Ukraine
DOI: https://doi.org/10.15407/fz64.02.019

Abstract

The activity of liver components of monooxygenase system (MOS) have been investigated in rats with tumor under conditions of ω-3 polyunsaturated fatty acids (PUFAs) administration. It was shown, that the intensive growth period of Guerin’s carcinoma is thus associated with cytochrome b5-reductase activity increased 2 times, and cytochrome b5 content decreased 2.8 times, and increased O2 - generation rate in reductive chain of MOS in comparison to control. We found NADPH-cytochrome P450-reductase activity decreased 2,4 times and cytochrome P450 content decreased 2,8 times in oxygenative chain of MOS of rats with transplanted Guerin’s carcinoma in the logarithmic phases of oncogenesis as compared with the control. The decrease of cytochrome P450 level in the liver microsomal fraction in the logarithmic phases of oncogenesis may be associated with its transition into an inactive form – cytochrome P420. ω-3 PUFAs (eicosapentaenoic acid and docosahexaenoic acid) administration before and after transplantation of Guerin’s carcinoma resulted in the decrease of NADH-cytochrome b5-reductase activity, increase of cytochrome b5 level, NADРH-cytochrome Р450 reductase activity and cytochrome P450 level in the liver microsomal fraction in the logarithmic phases of oncogenesis as compared with the tumor-bearing rats. We have established the correction of reductive and oxygenative chain of MOS under ω-3 PUFAs administration prior Guerin’s carcinoma injection as compared with the tumor-bearing rats. Yet the changes are less pronounced than those of the group receiving ω-3 PUFAs both prior to and post-Guerin’s carcinoma implantation. ω-3 PUFAs administration post-Guerin’s carcinoma injection does not influence on activity of liver MOS components of tumor-bearing rats.

Keywords: cytochrome P450; cytochrome b5; NADPH-cytochrome P450-reductase; NADH-cytochrome b5-reductase; ω-3 polyunsaturated fatty acids; microsomal fraction; liver, rats.

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