The changes of cardiac hemodynamics against the background of ischemia-reperfusion in 40 male and female white rats and their relationship to the level of hydrogen sulfide (H2S) in the blood serum of rats are studied. In the model of ischemiareperfusion we evaluated the parameters of cardiac hemodynamics (microcirculation coefficient, left ventricle oxygen saturation, pressure in right and left ventricle) and content of hydrogen sulfide in the blood serum. It was found that in males background level of microcirculation ratio in the left ventricle and the content of H2S in the blood serum is significantly lower (by 9-18%) than females. Under experimental occlusion of left coronary artery H2S level reduce in blood serum is registered (in males by 64.5%, in females by 51.1%), which is accompanied by inhibition of microcirculation and oxygen supply of cardiomyocytes of the left ventricle (microcirculation rate and oxygen saturation are reduced), by weakening of its contractive capacity and violation of intraventricular hemodynamics (pressure is reduced in left and right ventricle). These changes were more pronounced in males than in females. Reperfusion of coronary vessels in animals of both sexes did not cause total recovery of H2S content in serum and cardiac hemodynamics indicators to background levels, indicating the formation of unrestored coronary circulation syndrome. Іnfusion of NaHS not only prevented ischemia of heart muscle, but also contributed to a more complete recanalization by improving effective perfusion in both males and females. Correlative analysis showed that H2S level in blood serum reveals direct links with microcirculation coefficient, left ventricle oxygen saturation, pressure in right and left ventricle (in males r = 0.54-0.62; in females r = 0.60-0.70). These data suggest an important role of H2S system in the regulation of microcirculation and cardiac hemodynamics in male and female rats on background of ischemia-reperfusion.