Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2018; 64(1): 34-39


V.P. Fylymonenko, A.L. Zagayko

    National University of Pharmacy, Kharkiv, Ukraine


Effect of quercetin, epigallocatechin gallate and polyphenol extract from grapes administration on the changes in cholesterol metabolism in the rat blood serum under insulin resistance was studied. The development of atherogenic dyslipoproteinemia has been revealed: the accumulation of total cholesterol (2.12 times) due to the increase of very low density lipoproteins cholesterol content (1.75 times), the decrease of high density lipoproteins cholesterol concentration (2.6 times) and the increase of the atherogenic index (8.8 times) in animals on a high-fructose diet. The use of all studied polyphenols normalized the indicators of cholesterol metabolism in a different degree. Polyphenolic grape extract showed more pronounced protective properties, obviously due to the variety of composition and, accordingly, the mechanisms of insulin resistance correction.

Keywords: insulin resistance; cholesterol metabolism; grape polyphenols; quercetin; epigallocatechin gallate.


  1. Zagajko AL, Voronina LM, Strel'chenko KV. Metabolic syndrome: mechanisms and prospects of antioxidant therapy: Monograph. Harkiv: Vyd-vo NfaU: Zoloti storinky. 2007. 216 p. [Ukrainian].
  3. Gallagher EJ, Leroith D, Karnieli E. The metabolic syndrome – from insulin resistance to obesity and diabetes. Med Clin North Am. 2011 Sep;95(5):855-73. doi: 10.1016/j.mcna.2011.06.001. PMID: 21855696. CrossRef  
  4. Khurana S, Venkataraman K, Hollingsworth A, Piche M, Tai TC. Polyphenols: Benefits to the Cardiovascular System in Health and in Aging. Nutrients. 2013 Oct; 5(10): 3779-3827. Published online 2013 Sep 26. CrossRef PubMed PubMedCentral
  6. Rasines-Perea Z, Teissedre P-L. Grape Polyphenols' Effects in Human Cardiovascular Diseases and Diabetes. Molecules. 2017; 22(1), 68: doi:10.3390/molecules22010068 CrossRef  
  7. Vinayagam R, Xu B. Antidiabetic properties of dietary flavonoids: a cellular mechanism review. Nutrition & Metabolism. 201512:60 doi: 10.1186/s12986-015- 0057-7.
  9. Hossain MK, Dayem AA, Han J, Yin Y, Kim K, Saha SK, Yang G-M, Choi HY, Cho S-G. Molecular Mechanisms of the Anti-Obesity and Anti-Diabetic Properties of Flavonoids. Int J Mol Sci. 2016 Apr; 17(4): 569. Published online 2016 Apr 15. doi: 10.3390/ijms17040569. PMCID: PMC4849025. CrossRef  
  10. Altaş M, Var A, Köse C; Özbilgin, K, Arı Z. Endothelial dysfunction in high fructose containing diet fed rats: Increased nitric oxide and decreased endothelin-1 levels in liver tissue. Dicle Medical Journal / Dicle Tip Dergisi; 2010; 37(3):193-8.
  12. Bezerra RM, Ueno M, Silva MS, Tavares DQ, Carvalho CR, Saad MJ. A high fructose diet affects the early steps of insulin action in muscle and liver of rats. J Nutr. 2000 Jun; 130(6):1531-5. PMID: 10827205. CrossRef PubMed
  14. Zhang DM, Jiao RQ, Kong LD. High Dietary Fructose: Direct or Indirect Dangerous Factors Disturbing Tissue and Organ Functions. Nutrients. 2017 Mar 29;9(4). pii: E335. doi: 10.3390/nu9040335. CrossRef  
  15. Yan L.-J. Pathogenesis of Chronic Hyperglycemia: From Reductive Stress to Oxidative Stress. J Diabetes Res. 2014; 2014: 137919. Published online 2014 Jun 16. doi:1155/2014/137919. PMCID: PMC4082845
  17. Vazquez Prieto MA, Bettaieb A, Rodriguez Lanzi C, Soto VC, Perdicaro DJ, Galmarini CR, Haj FG, Miatello RM, Oteiza PI. Catechin and quercetin attenuate adipose inflammation in fructose-fed rats and 3T3-L1 adipocytes. Mol Nutr Food Res. 2015 Apr; 59(4):622-33. doi:10.1002/mnfr.201400631 CrossRef  
  18. Henagan TM, Cefalu WT, Ribnicky DM, Noland RC, Dunville K, Campbell WW, Stewart LK, Forney LA, Gettys TW, Chang JS, Morrison CD. In vivo effects of dietary quercetin and quercetin-rich red onion extract on skeletal muscle mitochondria, metabolism, and insulin sensitivity. Genes Nutr. 2015 Jan; 10(1): 2. Published online 2014 Dec 27. doi: 10.1007/s12263-014-0451-1. PMCID: PMC4277553. CrossRef  
  19. Chen Y-K, Cheung C, Reuhl KR., Liu AB, Lee M-J, Lu Y-P, Yang CS. Effects of Green Tea Polyphenol (-)-Epigallocatechin-3-gallate on a Newly Developed High-fat/Western-style Diet-induced Obesity and Metabolic Syndrome in Mice. J Agric Food Chem. 2011 Nov 9; 59(21): 11862-71. Published online 2011 Oct 18. doi: 10.1021/jf2029016 PMCID: PMC3243651 NIHMSID: NIHMS332936. CrossRef  
  20. Legeay S, Rodier M, Fillon L, Faure S, Clere N. Epigallocatechin Gallate: A Review of Its Beneficial Properties to Prevent Metabolic Syndrome. Nutrients. 2015 Jul 7; 7(7):5443-68. doi: 10.3390/nu7075230. PMID: 26198245 PMCID: PMC4517007. CrossRef  
  21. Kim Y, Keogh JB, Clifton PM. Polyphenols and Glycemic Control. Nutrients. 2016 Jan; 8(1): 17. Published online 2016 Jan 5. doi: 10.3390/nu8010017. PMCID: PMC4728631 CrossRef  
  22. Downing LE, Heidker RM, Caiozzi GC, Wong BS, Rodriguez K, Del Rey F, Ricketts ML. A Grape Seed Procyanidin Extract Ameliorates Fructose-Induced Hypertriglyceridemia in Rats via Enhanced Fecal Bile Acid and Cholesterol Excretion and Inhibition of Hepatic Lipogenesis. PLoS One. 2015 Oct 12;10(10):e0140267. doi: 10.1371/journal.pone.0140267. eCollection 2015. PMID: 26458107 PMCID: PMC4601771. CrossRef  
  23. Zagayko AL, Kravchenko GB, Krasilnikova OA, Ogai YO. Grape Polyphenols Increase the Activity of HDL Enzymes in Old and Obese Rats. Oxid Med Cell Longevity. Volume 2013 (2013), Article ID 593761, 7 pages. CrossRef  
  24. Doan KV, Ko CM, Kinyua AW, Yang DJ, Choi YH, Oh IY, Nguyen NM, Ko A, Choi JW, Jeong Y, Jung MH, Cho WG, Xu S, Park KS, Park WJ, Choi SY, Kim HS, Moh SH, Kim KW. Gallic acid regulates body weight and glucose homeostasis through AMPK activation. Endocrinology. 2015 Jan; 156(1):157-68. doi: 10.1210/ en.2014-1354. PMID: 25356824.
  26. Rafieirad M, Chehardacheric SV, Nezhad NN. Hypolipidemic effects of gallic acid in diabetic rats. Journal of pharmaceutical and biomedical sciences. J Pharm Biomed Sci. 2013 July; 32(32): 1309-1312.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2021.