CHANGES OF ONGOING ACTIVITY AND ELECTROPHYSIOLOGICAL CHARACTERISTICS OF THE RAT RETINAL GANGLION CELLS AT DIABETES MELLITUS
N.Ya. Martyniuk, V.Yu. Maslov, H.E. Purnyn, S.A. Fedulova, N.S. Veselovsky
Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.01.003
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Abstract
Changes of electrophysiological characteristics and ongoing
activity parameters of the retinal ganglion cells (RGCs) on
isolated rat retina were studied 3-4 weeks after streptozotocin
(STZ) injection (80 mg/kg). It was shown that following
electrophysiological characteristics significantly decreased:
density of sodium current on 35 %, -0,84 ± 0,14 nA/pF in
control and -0,55 ± 0,08 nA/pF at the STZ-induced diabetes
mellitus (DM) and potassium current on 36 %, 1,36 ± 0,16 nA/
pF in control and 0,87 ± 0,14 nА/pF at DM, resting membrane
potential on 10 %, -59 ± 1 mV in control and -53 ± 1 mV at
DM. The mean frequency of ongoing postsynaptic currents
reduced on 72 %, 2,5 ± 0.3 s-1 in control and 0,7 ± 0,1 s-1
at DM. The data suggest that pathological changes of the
electrophysiological characteristics in RGCs appear at early
stage of experimentally-induced DM.
Keywords:
retinal ganglion cells; STZ-induced diabetes; voltage-dependent currents; postsynaptic currents.
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