CHANGES OF ONGOING ACTIVITY AND ELECTROPHYSIOLOGICAL CHARACTERISTICS OF THE RAT RETINAL GANGLION CELLS AT DIABETES MELLITUS
N.Ya. Martyniuk, V.Yu. Maslov, H.E. Purnyn, S.A. Fedulova, N.S. Veselovsky
Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz64.01.003
Abstract
Changes of electrophysiological characteristics and ongoing
activity parameters of the retinal ganglion cells (RGCs) on
isolated rat retina were studied 3-4 weeks after streptozotocin
(STZ) injection (80 mg/kg). It was shown that following
electrophysiological characteristics significantly decreased:
density of sodium current on 35 %, -0,84 ± 0,14 nA/pF in
control and -0,55 ± 0,08 nA/pF at the STZ-induced diabetes
mellitus (DM) and potassium current on 36 %, 1,36 ± 0,16 nA/
pF in control and 0,87 ± 0,14 nА/pF at DM, resting membrane
potential on 10 %, -59 ± 1 mV in control and -53 ± 1 mV at
DM. The mean frequency of ongoing postsynaptic currents
reduced on 72 %, 2,5 ± 0.3 s-1 in control and 0,7 ± 0,1 s-1
at DM. The data suggest that pathological changes of the
electrophysiological characteristics in RGCs appear at early
stage of experimentally-induced DM.
Keywords:
retinal ganglion cells; STZ-induced diabetes; voltage-dependent currents; postsynaptic currents.
References
- World Health Organization. Global status report on noncommunicable diseases. Geneva. 2014.
- Kern TS, Barber AJ. Retinal ganglion cells in diabetes, J Physiol. 2008;586(18):4401-8.
CrossRef
PubMed PubMedCentral
- Gastinger MJ, Kunselman AR, Conboy EE, Bronson SK, Barber AJ. Dendrite remodeling and other abnormalities in the retinal ganglion cells of Ins2 Akita diabetic mice. Invest Ophthalmol Vis Sci. 2008;49(6):2642-53.
CrossRef
PubMed
- Rerup CC. Drugs producing diabetes through damage of the insulin secreting cells. Pharmacol Rev.1970;22(4):485-518.
PubMed
- Gajdosík A, Gajdosíková A, Stefek M, Navarová J, Hozová R. Streptozotocin-induced experimental diabetes in male Wistar rats. Gen Physiol Biophys. 1999 Oct;18:54-62.
PubMed
- Cortright RN, Collins HL, Chandler MP, Lemon PW, DiCarlo SE. Diabetes reduces growth and body composition more in male than in female rats. Physiol Behav. 1996;60(5):1233-38.
CrossRef
- Brown RA, Walsh MF, Ren J. Influence of gender and diabetes on vascular and myocardial contractile function. Endocr Res. 2001 Nov;27(4):399-408.
CrossRef
PubMed
- Ramsey DJ, Ripps H, Qian H. An electrophysiological study of retinal function in the diabetic female rat. Invest Ophthalmol Vis Sci. 2006;47(11):5116-24.
CrossRef
PubMed
- Guenther E, Schmid S, Reiff D. Maturation of intrinsic membrane properties in rat retinalganglion cell. Vision Res.1999;39(15):2477-84.
CrossRef
- Kuznetsov KI, Maslov VYu, Fedulova SA, Veselovsky NS. Calcium signals induced by tonic firing in the rat retinal ganglion cells. Fiziol Zh. 2011; 57(1): 3-8.
CrossRef
- Martynyuk NYa, Purnyn' EE, Fedulova SA. Effect of a blocker of nicotine acetylcholine receptors on excitatory postsynaptic currents in ganglion cells of the rat retina. Neurophysiol. 2014;46(6):516-20.
CrossRef
- O'Brien BJ, Isayama T, Richardson R, Berson DM. Intrinsic physiological properties of cat retinal ganglion cells. J Physiol. 2002;538(3):787-802.
CrossRef
PubMed PubMedCentral
- Kerschensteiner D. Glutamatergic Retinal Waves. Front Neural Circuits. 2016; 10(38).
CrossRef
- Farajian R, Pan F, Akopian A, Völgyi B, Bloomfield SA. Masked excitatory crosstalk between the ON and OFF visual pathways in the mammalian retina. J Physiol. 2011;589(18):4473-89.
CrossRef
PubMed PubMedCentral
- Ambati J, Chalam KV, Chawla DK, D'Angio CT, Guillet EG, Rose SJ, et al. Elevated gamma-aminobutyric acid, glutamate, and vascular endothelial growth factor levels in the vitreous of patients with proliferative diabetic retinopathy. Arch Ophthalmol. 1997;115(9):1161-66.
CrossRef
PubMed
- Kowluru RA, Engerman RL, Case GL, Kern TS. Retinal glutamate in diabetes and effect of antioxidants. Neurochem Int. 2001;38(5):385-90.
CrossRef
- Lieth E, Barber AJ, Xu B, Dice C, Ratz MJ, Tanase D, et al. Glial reactivity and impaired glutamate metabolism in short-term experimental diabetic retinopathy. Diabetes. 1998;47(5):815-20.
CrossRef
PubMed
- Yu J, Wang L, Weng S, Yang X, Zhang D, Zhong YM. Hyperactivity of ON-type retinal ganglion cells in streptozotocin- induced diabetic mice. PLoS One. 2013;8(9):e76049.
CrossRef
PubMed PubMedCentral
- Ozawa S, Kamiya H, Tsuzuki K. Glutamate receptors in the mammalian central nervous system. Prog Neurobiol. 1998;54(5):581-618.
CrossRef
- Stern P, Edwards FA, Sakmann B. Fast and slow components of unitary EPSCs on stellate cells elicited by focal stimulation in slices of rat visual cortex. J Physiol. 1992;449:247-78.
CrossRef
PubMed PubMedCentral
- Protti DA, Gerschenfeld HM, Llano I. GABAergic and glycinergic IPSCs in ganglion cells of rat retinal slices. J Neurosci. 1997;17(16):6075-85.
CrossRef
PubMed
- Brombas A, Kalita-de Croft S, Cooper-Williams EJ, Williams SR. Dendro-dendritic cholinergic excitation controls dendritic spike initiation in retinal ganglion cells. Nature Commun. 2017; 8:15683.
CrossRef
PubMed PubMedCentral
- Kleppe IC, Robinson HP. Determining the activation time course of synaptic AMPA receptors from openings of colocalized NMDA receptors. Biophys J. 1999;77(3): 1418-27.
CrossRef
|