Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(6): 72-83


STRUCTURAL AND FUNCTIONAL FEATURES OF SMALL INTESTINE MUCOUS DEPENDING ON QUANTITATIVE AND QUALITATIVE MICROBIOTA COMPOSITION

A.V. Putnikov, I.М. Vareniuk, N.М. Roslova, Y.V. Ноlоta, T.V. Dovbynchuk, T.M. Chervinska, T.М. Sergiychuk,M.Е. Dzerzhynsky, G.M. Тоlstanova

    Taras Shevchenko National University of Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz63.06.072

Abstract

In this work the morphological and functional features of the small intestine mucosa depending on micriobiota of parietal small intestine in long term use antibiotic ceftriaxone and multiprobiotic or their co-administration were studied. Study was done on white laboratory rats, which were administrated daily with ceftriaxone antibiotic alone or along with multiprobiotic within 14 days, the autopsy was done on the 15th, 29th and 72nd days of the experiment. It was shown that antibiotic use caused morphological and functional state changes of the small intestine mucosa of rats, accompanied by hypertrophy of goblet cells and mast cell degranulation. The consequences of the antibiotic use remain even in periods after and not completely disappear at 29th and 72nd days of experiment. Morphofunctional changes of small intestinal mucosa constrained with changes in composition of intestinal microbiota that was characterized by the height of conditionally-pathogenic microbiota, in particular, Staphylococcus aureus and Candida. A chart of simultaneous application and abolition of multiprobiotic with an antibiotic is ineffective for the removal of negative consequences of introduction of antibiotic. Application of multiprobiotic together with an antibiotic and continuation of application of the multiprobiotic alone, or administration of multiprobiotic after antibiotic cessation are considerably more effective comparatively with the first mode and allows to decrease or fully remove violation of the functional state of mucous membrane of small intestine.

Keywords: Small intestine; antibioticotherapy; mucosal morphofunctional state; microbiota; probiotic

References

  1. Kucherenko NP. Study of morphological changes of rat intestine under prolonged action of antibioticotherapy. Ukr Med Almanac. 2003;6(2):167 - 71. [Russian].
  2.  
  3. Korneichuk OP. Microbiocenosis of patients with chronic colitis intestines and colonic tumors. Exp and Clin Physiol & biochemistry. 2000;3:125 - 27.
  4.  
  5. Groschwitz KR, Hogan SP. Intestinal barrier function: molecular regulation and disease pathogenesis. J Allergy Clin Immunol. 2009;124:3 - 20. CrossRef PubMed PubMedCentral
  6.  
  7. Fries W, Belvedere A, Vetrano S. Sealing the broken barrier in IBD: intestinal permeability, epithelial cells and junctions. Curr Drug Targets. 2013;14:1460 - 470. CrossRef PubMed
  8.  
  9. Swindle EJ, Collins JE, Davies DE. Breakdown in epithelial barrier function in patients with asthma: identification of novel therapeutic approaches. J Allergy Clin Immunol. 2009;124:23 - 34. CrossRef PubMed
  10.  
  11. Leung DY. New insights into atopic dermatitis: role of skin barrier and immune dysregulation. Allergol Int. 2013;62:151 - 61. CrossRef PubMed
  12.  
  13. Simeoli R, Raso G, Lama A, Pirozzi C, Santoro A, Guida F, Sanges M, Aksoy E, Calignano A, D'Arienzo A, Meli R. Preventive and Therapeutic Effects of Lactobacillus Paracasei B21060–Based Synbiotic Treatment on Gut Inflammation and Barrier Integrity in Colitic Mice. J Nutr. 2015;145:1202 - 10. CrossRef PubMed
  14.  
  15. Dovbynchuk T, Zakordonets L, Putnikov A, Vareniuk I, Tiapko O, Roslova N, Sergiychuk T, Lynchak O., Dzerzhynsky M, Beregova T, ??lstanova G. Net water transport via rat colon epithelium under the experimental dysbiosis. Fiziol Zh. 2015;61(6):76 - 85. [Ukrainian]. CrossRef PubMed
  16.  
  17. Yanfei Chen, Feng Ji, Jing Guo, Ding Shi, Daiqiong Fang & Lanjuan Li. Dysbiosis of small intestinal microbiota in liver cirrhosis and its association with etiology. Sci Rep. 2016;6(34055):1012 - 19. CrossRef  
  18. Lin HC, et al. Small intestinal bacterial overgrowth: a framework for understanding irritable bowel syndrome. JAMA. 2004;292(7):852 - 58. CrossRef PubMed
  19.  
  20. Dukowicz AC, Lacy BE, Levine GM. Small Intestinal Bacterial Overgrowth: A Comprehensive Review. Gastroenterol & Hepatol. 2007;3(2):112 - 22.
  21.  
  22. Kuhn KA, Stappenbeck TS. Peripheral education of the immune system by the colonic microbiota. Semin Immunol. 2013;25(5):364 - 69. CrossRef PubMed PubMedCentral
  23.  
  24. Hajela N, Nair G, Abraham P, Ganguly P, Nirmal K. Health impact of probiotics - vision and opportunities. Gut Pathog. 2012;4(1):1. CrossRef PubMed PubMedCentral
  25.  
  26. Aihara E, Engevik KA, Montrose MH. Trefoil Factor Peptides and Gastrointestinal Function. Annu Rev Physiol. 2017;79:357 - 80. CrossRef PubMed PubMedCentral
  27.  
  28. Makkink M, Schwerbrock N, Mähler M et al. Fate of goblet cells in experimental colitis. Dig Dis Sci.2002;47:2286 - 297. CrossRef PubMed
  29.  
  30. McDole JR, Wheeler LW, McDonald KG, Wang B, Konjufca V, Knoop KA, Newberry RD, and Miller MJ. Goblet cells deliver luminal antigen to CD103+ DCs in the small intestine. Nature. 2012;483(7389):345 - 49. CrossRef PubMed PubMedCentral
  31.  
  32. Johansson M., Ambort D., Pelaseyed T., et al. Composition and functional role of the mucus layers in the intestine. Cell Mol Life Sci. 2011;68:3535 - 641. 18. Ng J., et al. Clostridium difficile toxin-induced inflammation and intestinal injury are mediated by the inflammasome. Gastroenterology. 2010;139:542 - 52. CrossRef PubMed
  33. Ng J., et al. Clostridium difficile toxin-induced inflammation and intestinal injury are mediated by the inflammasome. Gastroenterology. 2010;139:542 - 52. CrossRef PubMed
  34.  
  35. Theriot CM, Bowman AA, Young VB. Antibiotic-Induced Alterations of the Gut Microbiota Alter Secondary Bile Acid Production and Allow for Clostridium difficile Spore Germination and Outgrowth in the Large Intestine. mSphere. 2016;6;1(1): e00045-15.
  36.  
  37. Heinsen FA, Knecht H, Neulinger S et al. Dynamic changes of the luminal and mucosa-associated gut microbiota duing and after antibiotic therapy with paromycin. Gut Microbes. 2015; 6(4):243 - 54. CrossRef PubMed PubMedCentral
  38.  
  39. Knoop KA, McDonald KG, Kulkarni DH, Newberry RD. Antibiotics promote inflammation through the translocation of native commensal colonic bacteria. Gut. 2016;65(7):1100 - 109. CrossRef PubMed PubMedCentral
  40.  
  41. Bleich M, Ecke D, Schwartz B et al. Effects of the carcinogen dimethylhydrazine (DMH) on the function of rat colonic crypts. Pflugers Arch. 1997;433:254 – 59. CrossRef PubMed
  42.  
  43. Bures J, Pejchal J, Kvetina J et al. Morphometric analysis of the porcine gastrointestinal tract in a 10-day highdose indomethacin administration with or without probiotic bacteria Escherichia coli Nissle 1917. Hum Exp Toxicol. 2011: 30(12):1955 - 962. CrossRef PubMed
  44.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.